FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Rnf220
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General Information
Symbol
Dmel\Rnf220
Species
D. melanogaster
Name
Ring finger protein 220
Annotation Symbol
CG4813
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039013
Gene Model Status
Current
Stock Availability
6 publicly available
Enzyme Name (EC)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
94C3-94C4
Sequence location
Recombination map
3-76
RefSeq locus
NT_033777 REGION:22724884..22726414
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables metal ion binding
inferred from electronic annotation with InterPro:IPR040178
(InterPro Project Members, 2004-)
enables ubiquitin protein ligase activity
inferred from sequence or structural similarity with UniProtKB:Q6PDX6
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN002298104
(GO Reference Genome Project, 2011-)
enables ubiquitin-protein transferase activity
inferred from electronic annotation with InterPro:IPR040178
(InterPro Project Members, 2004-)
enables zinc ion binding
inferred from sequence model
(Ying et al., 2011)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in protein ubiquitination
inferred from biological aspect of ancestor with PANTHER:PTN002298104
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:Q6PDX6
(Gene Ontology Curators, 2002-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from sequence or structural similarity with UniProtKB:Q6PDX6
(Gene Ontology Curators, 2002-)
located_in nucleus
inferred from sequence or structural similarity with UniProtKB:Q5VTB9
(Gene Ontology Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
ubiquitin protein ligase activity
Summaries
Gene Group (FlyBase)
UNCLASSIFIED RING DOMAIN UBIQUITIN LIGASES -
This group comprises RING domain ubiquitin ligases that do not classify under other groups in FlyBase.
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rnf220 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VCV3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.47

Gene model reviewed during 5.53

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rnf220-RA
FBtr0084326
NM_142831
1531
398
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Rnf220-PA
FBpp0083719
43.9
398
5.45
Q9VCV3
NP_651088
AAF56052
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rnf220 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.83

Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rnf220 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rnf220
Transgenic constructs containing regulatory region of Rnf220
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3R)Exel6192
(Ryder, 2004.4.26)
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (8)
Hsap\RNF220
12 of 14
Yes
Yes
Hsap\LONRF1
1 of 14
No
No
Hsap\PHRF1
1 of 14
No
No
Hsap\RNF5
1 of 14
No
No
1  
Hsap\RNF166
1 of 14
No
Yes
Hsap\RNF180
1 of 14
No
Yes
Hsap\TRIM10
1 of 14
No
Yes
Hsap\TRIM26
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Rnf220
8 of 14
Yes
Yes
Rnor\Dtx3l
1 of 14
No
Yes
Rnor\Rnf5
1 of 14
No
No
Rnor\Rnf166
1 of 14
No
Yes
Rnor\Rnf181
1 of 14
No
No
Rnor\Trim26
1 of 14
No
Yes
Mus musculus (laboratory mouse) (7)
Mmus\Rnf220
11 of 14
Yes
Yes
Mmus\Rnf5
1 of 14
No
No
Mmus\Rnf10
1 of 14
No
No
Mmus\Rnf166
1 of 14
No
Yes
Mmus\Rnf170
1 of 14
No
Yes
Mmus\Rnf185
1 of 14
No
No
Mmus\Trim26
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (9)
Xtro\rnf220.1
6 of 13
Yes
Yes
Xtro\XB22041735
2 of 13
No
Yes
Xtro\pou4f3
1 of 13
No
No
Xtro\rnf4
1 of 13
No
No
Xtro\rnf180
1 of 13
No
Yes
Xtro\rnf181
1 of 13
No
No
Xtro\rnf220.2
1 of 13
No
Yes
Xtro\trim69.1
1 of 13
No
No
Xtro\xnf7
1 of 13
No
No
Danio rerio (Zebrafish) (8)
Drer\rnf220a
11 of 14
Yes
Yes
Drer\rnf220b
5 of 14
No
Yes
Drer\amfra
1 of 14
No
No
Drer\cop1
1 of 14
No
Yes
Drer\lonrf1
1 of 14
No
No
Drer\rnf40
1 of 14
No
No
Drer\rnf180b
1 of 14
No
Yes
Drer\toporsa
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\rnf-5
1 of 14
Yes
No
Cele\Y4C6A.3
1 of 14
Yes
No
Cele\Y47G6A.31
1 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (3)
Agam\AgaP_AGAP003744
12 of 12
Yes
Yes
Agam\AgaP_AGAP011188
2 of 12
No
No
Agam\AgaP_AGAP007458
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (7)
Atha\AT1G18760
1 of 13
Yes
No
Atha\AT1G18770
1 of 13
Yes
No
Atha\AT1G51930
1 of 13
Yes
No
Atha\AT3G10910
1 of 13
Yes
No
Atha\AT3G58030
1 of 13
Yes
No
Atha\AT5G46650
1 of 13
Yes
No
Atha\ATL6
1 of 13
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Rnf220. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (5)
tey
3 of 13
CG10916
1 of 13
CG33552
1 of 13
elfless
1 of 13
Sce
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Rnf220HMJ23135
model of  hypomyelinating leukodystrophy 23
CEC
(Sferra et al., 2021)
CEA
(Sferra et al., 2021)
Potential Models Based on Orthology ( 1 )
Human Ortholog
Disease
Evidence
References
RNF220; ring finger protein 220
model of  hypomyelinating leukodystrophy 23
IEA
(FlyBase, 2019-)
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RNF220; ring finger protein 220
12 of 14
616136
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
Interaction Browsers
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-76
Cytogenetic map
94C3-94C4
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
94C3-94C4
Limits computationally determined from genome sequence between P{lacW}GclmL0580 and P{EP}hhEP3521
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location

3-76

(FlyBase, 2016)

3-78.3

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (6)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
      Other Comments
      Relationship to Other Genes
      Source for database merge of
      Additional comments
      Nomenclature History
      Source for database identify of

      Source for identity of: Rnf220 CG4813

      (Sferra et al., 2021)
      Nomenclature comments
      Etymology

      The gene is named 'Rnf220' based on the closest human ortholog.

      (Sferra et al., 2021)
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      CG4813
      (Frasch et al., 2023, Tang et al., 2023, Hu et al., 2017.6.13, Japanese National Institute of Genetics, 2012.5.21, Du et al., 2011, Ying et al., 2011, Perkins et al., 2009)
      RNF220
      (Sferra et al., 2021)
      Rnf220
      Name Synonyms
      Ring finger protein 220
      ring finger protein 220
      Secondary FlyBase IDs
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 41 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        References (33)