FlyBase Gene Report: Dmel\Sbat

General Information

Symbol

Dmel\Sbat

Species

D. melanogaster

Name

Sabbat

Annotation Symbol

CG31950

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051950

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Auxiliary component of the N-terminal acetyltransferase C (NatC) complex which catalyzes acetylation of N-terminal methionine residues (By similarity). May have an accessory function in the survival motor neuron (SMN) complex (PubMed:28949413, PubMed:30563832). (UniProt, Q8IPZ7)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

23C1-23C1

Sequence location

2L:2,878,344..2,879,001 [+]

Recombination map

2-8

RefSeq locus

NT_033779 REGION:2878344..2879001

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables RNA binding

inferred from electronic annotation with InterPro:IPR047575

(InterPro Project Members, 2004-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of NatC complex

inferred from direct assay

(Varland et al., 2023)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of NatC complex

inferred from electronic annotation with InterPro:IPR034110

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

N-TERMINAL ACETYLTRANSFERASE C COMPLEX

N-TERMINAL ACETYLTRANSFERASE C COMPLEX - TESTIS-SPECIFIC VARIANT

Protein Family (UniProt)

Belongs to the snRNP Sm proteins family. (Q8IPZ7)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

N-TERMINAL ACETYLTRANSFERASE C COMPLEX -: The N-terminal acetyltransferase C (NatC) complex catalyzes the acetylation of the N-terminal methionine of polypeptides with Met-Leu-, Met-Phe-, Met-Ile- and Met-Trp- N termini. (Adapted from PMID:19660096, PMID:22405572 and FBrf0234437).
N-TERMINAL ACETYLTRANSFERASE C COMPLEX - TESTIS-SPECIFIC VARIANT -

Protein Function (UniProtKB)

(UniProt, Q8IPZ7)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sbat for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IPZ7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sbat-RA

FBtr0077630

NM_164512

482

121

Sbat-RB

FBtr0332457

NM_001273042

658

121

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sbat-PA

FBpp0077315

13.4

121

5.67

FBpp0304730

13.4

121

5.67

Q8IPZ7

NP_001259971

AGB92508

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

121 aa isoforms: Sbat-PA, Sbat-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of Naa35, Sbat/Naa38 and Naa30A (PubMed:37891180). Interacts with Smn and Hez; along with Hez and Vlet, may form an accessory subcomplex involved in SMN complex function (PubMed:28949413).

(UniProt, Q8IPZ7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q8IPZ7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sbat using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

5.06

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of NatC complex

inferred from direct assay

(Varland et al., 2023)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sbat in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sbat

View exonic expression by tissue for Dmel\Sbat

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

319043/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051950

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051950

Flygut - An atlas of the Drosophila adult midgut

FBgn0051950

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sbat

Transgenic constructs containing regulatory region of Sbat

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 11 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sbat^GD11145

viable, with Scer\GAL4^Mef2.PR

Sbat^GD11145

viable, with Scer\GAL4^pnr-MD237

Sbat^GD11145

viable, with Scer\GAL4^tin.CΔ4

Sbat^GD11145

Sterility

Allele

male fertile, with Scer\GAL4^bam.PU

Sbat^GD11145

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\NAA38

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Naa38

12 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (16)

Mmus\Naa38

11 of 14

Yes

Mmus\Snrpb

2 of 14

Mmus\Snrpn

2 of 14

Mmus\Clca3b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\naa38

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\naa38

11 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\natc-3

4 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (13)

Agam\AgaP_AGAP013238

11 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (21)

Atha\AT4G18372

7 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (12)

Scer\LSM5

2 of 13

Yes

Scer\MAK31

2 of 13

Yes

Scer\SMB1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\naa38

7 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sbat. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

SmB

3 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

SmE

1 of 13

SNRPG

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NAA38; N-alpha-acetyltransferase 38, NatC auxiliary subunit

12 of 14

617990

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sbat - Gem3

two hybrid

(Lanfranco et al., 2017)

Sbat - Hez

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Lanfranco et al., 2017)

Sbat - SmB

two hybrid

(Lanfranco et al., 2017)

Sbat - SmD2

two hybrid

(Lanfranco et al., 2017)

Sbat - Smn

anti tag coimmunoprecipitation, anti tag western blot

(Lanfranco et al., 2017)

Sbat - Vlet

two hybrid

(Lanfranco et al., 2017)

Sbat - lms

two hybrid

(Hu et al., 2017.6.13)

Sbat - wmd

two hybrid

(Lanfranco et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q8IPZ7 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051950

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-8

Cytogenetic map

23C1-23C1

Sequence location

2L:2,878,344..2,879,001 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

23C1-23C1

Limits computationally determined from genome sequence between P{PZ}oho23B⁰³⁵⁷⁵ and P{PZ}lilli⁰⁰⁶³²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-8

(FlyBase, 2016)

2-6.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

55161

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03836}attP40

FlyORF

F002436

M{UAS-Sbat.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

2LG-1659

y² VhaAC39-1^cho-1 v¹; P{U6-Sbat.NIG.sgRNA}attP40/CyO

VDRC

v34750

w¹¹¹⁸; P{GD11145}v34750

v34751

w¹¹¹⁸; P{GD11145}v34751

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI06540

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051950

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

EC38964

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051950

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

CG31950/Sabbat is orthologous to human Naa38, not Gemin7.

(Matera et al., 2019)

New annotation (CG31950) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sbat CG31950

(Lanfranco et al., 2017)

Nomenclature comments

Etymology

Named 'Sabbat', the 7th day of the week, in recognition of its orthology to human Gemin7.

(Lanfranco et al., 2017)

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG31950

(National Institute of Genetics Fly Stocks, 2022-, Matera et al., 2019, Hu et al., 2017.6.13, Lanfranco et al., 2017, Ni et al., 2010.12.1)

Gem7

(Lanfranco et al., 2017)

Sbat

(Lanfranco et al., 2017)

dNAA38

(François et al., 2023)

Name Synonyms

Gemin7

(Lanfranco et al., 2017)

Sabbat

(Matera et al., 2019, Lanfranco et al., 2017)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

319043

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IPZ7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8IPZ7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IPZ7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

319043/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051950

FlyMine - An integrated database for Drosophila genomics

FBgn0051950

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31950

MARRVEL_MODEL - MARRVEL (model organism gene)

319043

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051950

DroID - A comprehensive database of gene and protein interactions.

FBgn0051950

DRSC - Results frm RNAi screens

FBgn0051950

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051950

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051950

Flygut - An atlas of the Drosophila adult midgut

FBgn0051950

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008128

References (24)

The following external sites may use different assemblies or annotations than FlyBase.

5.06

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