Sm B, Small ribonucleoprotein particle protein B
Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
199 (aa); 25 (kD observed); 25 (kD predicted)
Component of the osk mRNP (PubMed:20570937). Interacts with stau and yps (PubMed:20570937). Interacts with csul (via the N-terminal region) (PubMed:17164419). Interacts with vls (PubMed:17164419). Interacts (methylated) with tud (PubMed:17164419). Interacts with Smn; this interaction may be favored by SmB methylation (PubMed:16753561). Interacts with the SMN complex (PubMed:18621711). Interacts with msk and Snup; these interactions are RNA-dependent (PubMed:23885126).
Symmetrically dimethylated on arginine residues present in the RG repeats by csul (or csul-vls complex) and Art7; methylation is not required for assembly and biogenesis of snRNPs, but required for pole plasm localization. There seems to be a specific role of two subsets of arginine residues in the C-terminal region: the subset containing Arg-112, Arg-130 and Arg-145 is required during embryogenesis for gonad formation, whereas the subset containing Arg-174, Arg-181, Arg-189 and Arg-196 is involved in pole cell formation by controlling polar granule maintenance at the posterior pole of the oocyte.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SmB using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\SmB in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: SmB l(2)SH0509
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
SmB nucleotide sequence is highly conserved with that for the murine and human SmB polypeptides.
Encodes a protein with 65% identity (80% similarity) to human and murine SmB polypeptides. Like these mammalian proteins is precipitated by human anti-Sm autoantibodies and by the Y12 anti-Sm murine monoclonal antibody.