FlyBase Gene Report: Dmel\Ccn

General Information

Symbol

Dmel\Ccn

Species

D. melanogaster

Name

Ccn

Annotation Symbol

CG32183

Feature Type

protein_coding_gene

FlyBase ID

FBgn0052183

Gene Model Status

Current

Stock Availability

87 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

dCCN

Key Links

Genomic Location

Cytogenetic map

74F1-75A1

Sequence location

3L:17,690,015..17,748,839 [+]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17690015..17748839

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables heparin binding

inferred from biological aspect of ancestor with PANTHER:PTN000142748

(GO Reference Genome Project, 2011-)

enables integrin binding

inferred from biological aspect of ancestor with PANTHER:PTN000142750

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN000142750

(GO Reference Genome Project, 2011-)

involved_in positive regulation of cell differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000142750

(GO Reference Genome Project, 2011-)

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000142748

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR043973

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in extracellular matrix

inferred from biological aspect of ancestor with PANTHER:PTN000142748

(GO Reference Genome Project, 2011-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000142750

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ccn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VVK3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ccn-RB

FBtr0075186

NM_168744

2778

470

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ccn-PB

FBpp0074951

53.1

470

6.96

Q9VVK3

NP_730294

AAF49308

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ccn using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ccn_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.73

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ccn in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ccn

View exonic expression by tissue for Dmel\Ccn

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0052183

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39972/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052183

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052183

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052183

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32183

Flygut - An atlas of the Drosophila adult midgut

FBgn0052183

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ccn

Transgenic constructs containing regulatory region of Ccn

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel9005

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Ccn^GD6155

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Ccn^GD6155

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Ccn^GD6155

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ccn^GD6155

viable, with Scer\GAL4^tin.CΔ4

Ccn^GD6155

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal copulation

Ccn^{MI03160-TG4.un}

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Ccn^GD6155

abnormal neurophysiology | larval stage

Ccn^{MI03160-TG4.un}

abnormal neurophysiology | larval stage, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

abnormal neurophysiology | larval stage (with Df(3L)ED4710)

Ccn^{MI03160-TG4.un}

decreased fecundity

Ccn^{MI03160-TG4.un}

decreased fecundity | recessive, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

decreased size | oogenesis

Ccn^{MI03160-TG4.un}

decreased size | oogenesis, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

decreased size | oogenesis (with Df(3L)ED4710)

Ccn^{MI03160-TG4.un}

some die during pupal stage, with Scer\GAL4^pnr-MD237

Ccn^GD6155

Phenotype manifest in

Allele

embryonic/larval neuromuscular junction

Ccn^{MI03160-TG4.un}

embryonic/larval neuromuscular junction, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

embryonic/larval neuromuscular junction (with Df(3L)ED4710)

Ccn^{MI03160-TG4.un}

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Ccn^GD6155

oocyte | absent

Ccn^{MI03160-TG4.un}

oocyte | absent, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

oocyte | absent (with Df(3L)ED4710)

Ccn^{MI03160-TG4.un}

ovary

Ccn^{MI03160-TG4.un}

ovary, with Scer\GAL4^{Ccn-MI03160-TG4.un}

Ccn^HMS02310

ovary (with Df(3L)ED4710)

Ccn^{MI03160-TG4.un}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\CCN2

6 of 14

Yes

Hsap\CCN1

4 of 14

Yes

Hsap\CCN3

4 of 14

Yes

Hsap\CCN4

4 of 14

Yes

Hsap\CCN5

4 of 14

Yes

Hsap\CCN6

4 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Ccn2

6 of 14

Yes

Rnor\Ccn1

5 of 14

Yes

Rnor\Ccn3

4 of 14

Yes

Rnor\Ccn4

4 of 14

Yes

Rnor\Ccn5

4 of 14

Yes

Rnor\Ccn6

4 of 14

Yes

Mus musculus (laboratory mouse) (6)

Mmus\Ccn2

6 of 14

Yes

Mmus\Ccn1

5 of 14

Yes

Mmus\Ccn3

4 of 14

Yes

Mmus\Ccn4

4 of 14

Yes

Mmus\Ccn5

4 of 14

Yes

Mmus\Ccn6

4 of 14

Yes

Xenopus tropicalis (Western clawed frog) (10)

Xtro\ccn2

5 of 13

Yes

Xtro\ccn5

4 of 13

Yes

Xtro\ccn6

4 of 13

Yes

Xtro\pcyox1

3 of 13

Yes

Xtro\ccn1

2 of 13

Yes

Xtro\ccn3

2 of 13

Yes

Xtro\ccn4

2 of 13

Yes

Xtro\ctgfl

2 of 13

Yes

Xtro\col27a1

1 of 13

Xtro\LOC100489839

1 of 13

Danio rerio (Zebrafish) (10)

Drer\ccn2a

6 of 14

Yes

Drer\ccn1

5 of 14

Yes

Drer\ccn1l1

5 of 14

Yes

Drer\ccn1l2

4 of 14

Yes

Drer\ccn2b

4 of 14

Yes

Drer\ccn4a

4 of 14

Yes

Drer\ccn4b

4 of 14

Yes

Drer\ccn5

4 of 14

Yes

Drer\ccn6

4 of 14

Yes

Drer\si:ch211-106h11.3

4 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP006908

11 of 12

Yes

Agam\AgaP_AGAP000893

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ccn. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

cmpy

1 of 13

sas

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

CCN2; cellular communication network factor 2

model of kyphomelic dysplasia

IEA

(FlyBase, 2019-)

model of spondyloepimetaphyseal dysplasia, Li-Shao-Li type

IEA

(FlyBase, 2019-)

CCN6; cellular communication network factor 6

model of progressive pseudorheumatoid arthropathy of childhood

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CCN2; cellular communication network factor 2

6 of 14

121009

CCN1; cellular communication network factor 1

4 of 14

602369

CCN3; cellular communication network factor 3

4 of 14

164958

CCN4; cellular communication network factor 4

4 of 14

603398

CCN5; cellular communication network factor 5

4 of 14

603399

CCN6; cellular communication network factor 6

4 of 14

603400

PROGRESSIVE PSEUDORHEUMATOID DYSPLASIA; PPRD

progressive pseudorheumatoid arthropathy of childhood

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65257

DroID - A comprehensive database of gene and protein interactions.

FBgn0052183

MIST (protein-protein) - An integrated Molecular Interaction Database

39972

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

74F1-75A1

Sequence location

3L:17,690,015..17,748,839 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

74F1-75A1

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (87)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Korea

11935

P{VT032409-GAL4}attP2

14799

P{VT032407-GAL4}attP2

15165

P{VT032404-GAL4}attP2

15443

P{VT032402-GAL4}attP2

15529

P{GMR52H04-GAL4}attP2

15531

P{GMR53H01-GAL4}attP2

17527

P{VT032417-GAL4}attP2

16284

P{GMR53G12-lexA}attP2

14210

P{VT032411-lexA::GADfl}attP40

VDRC

v101518

P{KK108951}VIE-260B

v101520

P{KK108959}VIE-260B

v203212

P{VT032411-GAL4}attP2

More stocks available...

Genomic Clones (40)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (8)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH26928

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0052183

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0052183

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Ccn function can have a profound affect on multiple aspects of neuronal development. If dCCN is misexpressed in either the optic lobe or photoreceptor neurons, we see defects in the fasciculation and targeting of the outgrowing processes.

(Certel and Thor, 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG13735 CG13736

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG13735 and CG13736 merged as CG32183 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Ccn CG32183

(Certel, 2004.2.23)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG13735

CG13736

CG32183

(Hosono et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Ringrose et al., 2003)

Ccn

(Chen et al., 2025, Pignataro et al., 2024, Ascencio et al., 2023, Corthals et al., 2023, Yang et al., 2023, Deshpande et al., 2022, Pang et al., 2021, Trevino et al., 2021, Gene Disruption Project members, 2018-, Gene Disruption Project members, 2015-, Ashwal-Fluss et al., 2014, Yan et al., 2014, Neumüller et al., 2011)

dCCN

(Garrett et al., 2023, Certel and Thor, 2004)

Name Synonyms

Ccn

Secondary FlyBase IDs

FBgn0036748
FBgn0040792

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39972

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VVK3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VVK3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VVK3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0052183

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39972/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052183

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052183

FlyMine - An integrated database for Drosophila genomics

FBgn0052183

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32183

MARRVEL_MODEL - MARRVEL (model organism gene)

39972

Linkouts

BioGRID - A database of protein and genetic interactions.

65257

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0052183

DroID - A comprehensive database of gene and protein interactions.

FBgn0052183

DRSC - Results frm RNAi screens

FBgn0052183

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ccn_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052183

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0052183

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32183

Flygut - An atlas of the Drosophila adult midgut

FBgn0052183

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0052183

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030877

MIST (protein-protein) - An integrated Molecular Interaction Database

39972

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (60)

The following external sites may use different assemblies or annotations than FlyBase.

-0.73

Report Sections