FlyBase Gene Report: Dmel\CheB42a

General Information

Symbol

Dmel\CheB42a

Species

D. melanogaster

Name

Chemosensory protein B 42a

Annotation Symbol

CG33348

Feature Type

protein_coding_gene

FlyBase ID

FBgn0053348

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Chemosensory protein B 42a (CheB42a) encodes a member of a family of small secreted proteins associated with the chemosensory system and is involved in male courtship behavior. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

42E1-42E1

Sequence location

2R:6,949,482..6,950,284 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6949482..6950284

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables channel regulator activity

inferred from direct assay

(Ben-Shahar et al., 2010)

enables transmembrane transporter binding

inferred from physical interaction with FLYBASE:ppk25; FB:FBgn0053349

(Ben-Shahar et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables lipid binding

inferred from sequence alignment with UniProtKB:P61916

(Starostina et al., 2009)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in detection of pheromone

inferred from expression pattern

(Starostina et al., 2009, Xu et al., 2002)

involved_in male courtship behavior

inferred from mutant phenotype

(Park et al., 2006)

involved_in response to pheromone

inferred from mutant phenotype

(Park et al., 2006)

involved_in sensory perception of chemical stimulus

inferred from expression pattern

(Xu et al., 2002)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Ben-Shahar et al., 2010, Starostina et al., 2009)

part_of protein-containing complex

inferred from physical interaction with FLYBASE:ppk25; FB:FBgn0053349

(Ben-Shahar et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from sequence model

(Xu et al., 2002)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Uncharacterised protein family DM11, Drosophila melanogaster

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CheB42a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q3ZAK6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

May be component of a dicistronic gene (FBrf0191875); available data inconclusive.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CheB42a-RA

FBtr0086168

NM_206043

693

198

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CheB42a-PA

FBpp0089406

22.5

198

8.68

Q3ZAK6

NP_995765

AAM70837

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Uncharacterised protein family DM11, Drosophila melanogaster

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CheB42a using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CheB42a_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.52

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

neuron

(Ben-Shahar et al., 2007)

adult stage | male

head

(Xu et al., 2002)

prothoracic metatarsus

(Xu et al., 2002)

prothoracic tarsal segment 2

(Xu et al., 2002)

prothoracic tarsal segment 3

(Xu et al., 2002)

prothoracic tarsal segment 4

(Xu et al., 2002)

prothoracic tarsal segment

Comment: NOT prothoracic tarsal segment 5

(Xu et al., 2002)

adult stage

prothoracic leg taste bristle | subset

(Starostina et al., 2009)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Xu et al., 2002)

(Xu et al., 2002)

(Ben-Shahar et al., 2007)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

prothoracic leg

(Starostina et al., 2009)

leg

(Ben-Shahar et al., 2007)

Additional Descriptive Data

CheB42a is expressed in distinct, nonoverlapping sets of taste sensilla on male front legs relative to CheB93a.

(Starostina et al., 2009)

RT-PCR and northern blots show that CheB42a is enriched in male appendages relative to head and body and female parts. In situ to late embryos detects expression in two cells at the anterior end thought to be neurons. It is expressed in the same cells as ppk25.

(Ben-Shahar et al., 2007)

Expression of both CheA29a and CheB42a is specific to the male front leg with expression in two or three sites per segment in tarsal segments 1-4. CheB42a expression was further localized to non-neuronal chemosensory sensilla. Low level expression of both genes was also detected in male heads.

(Xu et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

prothoracic leg

(Xu et al., 2002)

thecogen cell of prothoracic leg taste bristle

(Park et al., 2006)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Xu et al., 2002)

(Xu et al., 2002)

Additional Descriptive Data

CheB42a is expressed in the sheath (thecogen) cells of a subset of gustatory sensilla in the prothoracic male legs. These same sensilla are also labelled by ScerGAL4^Gr68a.PB and ScerGAL4^Pbprp2.PS, although expression is found in different cells of each sensilla.

(Park et al., 2006)

CheB42a protein is present in extracts from male front legs starting at one day after eclosion.

(Xu et al., 2002)

CheB42a protein isdetected only in male front legs. Expression can be detected in late pupae and 1-7 day old adults, with highest expression at day 3 but not in pharate adult legs.

(Xu et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Ben-Shahar et al., 2010, Starostina et al., 2009)

part_of protein-containing complex

inferred from physical interaction with FLYBASE:ppk25; FB:FBgn0053349

(Ben-Shahar et al., 2010)

Expression Deduced from Reporters

Reporter: P{CheB42a-GAL4.X}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

multidendritic neuron

(Ben-Shahar et al., 2007)

external sensory organ

(Ben-Shahar et al., 2007)

adult stage | male

prothoracic leg taste bristle | subset

(Park et al., 2006, Xu et al., 2002)

prothoracic metatarsus

(Xu et al., 2002)

prothoracic tarsal segment 2

(Xu et al., 2002)

prothoracic tarsal segment 3

(Xu et al., 2002)

prothoracic tarsal segment 4

(Xu et al., 2002)

prothoracic tibia

(Xu et al., 2002)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CheB42a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CheB42a

View exonic expression by tissue for Dmel\CheB42a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768721/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053348

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053348

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CheB42a

Transgenic constructs containing regulatory region of CheB42a

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)Δ5-2

(Lin et al., 2005)

Df(2R)Δ5-22

(Lin et al., 2005)

Df(2R)Δ42E

(Lin et al., 2005)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CheB42a^GD16072

Other Phenotypes

Allele

abnormal behavior

CheB42a^Δ5-22

abnormal courtship behavior

abnormal taste perception

CheB42a^Δ5-68

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CheB42a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CheB42a - ppk25

anti tag coimmunoprecipitation, western blot

(Ben-Shahar et al., 2010)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CheB42a

suppressible

tra

(Park et al., 2006)

CheB42a

enhanceable

tra

(Park et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0053348

MIST (genetic) - An integrated Molecular Interaction Database

2768721

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42E1-42E1

Sequence location

2R:6,949,482..6,950,284 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42E1-42E1

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42D-42D

(Pikielny, 2001.7.13)

42E-42E

(determined by in situ hybridisation)

(Xu et al., 2002)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

66677

w^*; P{CheB42a-GAL4.X}1, P{UAS-GFP.U}2

66678

w^*; P{CheB42a-GAL4.X}2, P{UAS-GFP.U}3

34078

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00590}attP2

FlyORF

F003168

M{UAS-CheB42a.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

HMS00590

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00590}attP2

VDRC

v106020

P{KK112784}VIE-260B

v51193

w¹¹¹⁸; P{GD16072}v51193

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP03603

Other clones

DPiM_IP03603

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053348

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053348

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Xu et al., 2002)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

CheB42a is required for normal gustatory response to female cuticular hydrocarbon pheromones that modulate male behaviour.

(Park et al., 2006)

Relationship to Other Genes

Source for database merge of

Additional comments

Source for identity of CheB42a CG15242 was sequence comparison ( date:020502 ).

(FlyBase Genome Annotators, 2004, FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: CheB42a CG15242

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG15242

CG33348

(Tsubouchi et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

CheB42

(Billeter and Levine, 2013)

CheB42A

(Lu et al., 2012)

CheB42a

(Huang et al., 2024, He et al., 2019, Shankar et al., 2015, Starostina et al., 2012, Tsubouchi et al., 2012, Ben-Shahar et al., 2010, Starostina et al., 2009, Ben-Shahar et al., 2007, Robin et al., 2007, Park et al., 2006, Svetec and Ferveur, 2005, Svetec et al., 2005, Xu et al., 2002)

Name Synonyms

CheB42a

Chemosensory protein B 42a

(Tsubouchi et al., 2012)

Secondary FlyBase IDs

FBgn0033120

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 25 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

2768721

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF399853

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q3ZAK6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q3ZAK6

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768721/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053348

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053348

FlyMine - An integrated database for Drosophila genomics

FBgn0053348

InterPro - A database of protein families, domains and functional sites

Uncharacterised protein family DM11, Drosophila melanogaster

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33348

MARRVEL_MODEL - MARRVEL (model organism gene)

2768721

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053348

DroID - A comprehensive database of gene and protein interactions.

FBgn0053348

DRSC - Results frm RNAi screens

FBgn0053348

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CheB42a_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0053348

MIST (genetic) - An integrated Molecular Interaction Database

2768721

References (37)

The following external sites may use different assemblies or annotations than FlyBase.

-0.52

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