FlyBase Gene Report: Dmel\Tpst

General Information

Symbol

Dmel\Tpst

Species

D. melanogaster

Name

Tyrosylprotein sulfotransferase

Annotation Symbol

CG32632

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086674

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

protein-tyrosine sulfotransferase (2.8.2.20)

Gene Summary

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides (By similarity). Has a role in protein secretion. (UniProt, Q9VYB7)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Tango13, Transport and Golgi organization 13, DmTPST

Key Links

Genomic Location

Cytogenetic map

12A4-12A7

Sequence location

X:13,563,823..13,593,224 [+]

Recombination map

1-44

RefSeq locus

NC_004354 REGION:13563823..13593224

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein-tyrosine sulfotransferase activity

inferred from direct assay

(Huang et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein-tyrosine sulfotransferase activity

inferred from electronic annotation with InterPro:IPR026634

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000300376

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in protein secretion

inferred from mutant phenotype

(Bard et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in Golgi apparatus

inferred from direct assay

(Bard et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN001765270

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED SULFOTRANSFERASES

Protein Family (UniProt)

Belongs to the protein sulfotransferase family. (Q9VYB7)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein-tyrosine sulfotransferase activity

L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine- [protein] + adenosine 3',5'-bisphosphate + H(+) (2.8.2.20)

RHEA 16801:

Summaries

Gene Group (FlyBase)

UNCLASSIFIED SULFOTRANSFERASES -: This group comprises sulfotransferases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides (By similarity). Has a role in protein secretion.

(UniProt, Q9VYB7)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tpst for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VYB7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Stop-codon suppression (UGA) postulated; FBrf0216884.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.13

Gene model reviewed during 5.44

Gene model reviewed during 5.43

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tpst-RB

FBtr0073803

NM_167374

2734

499

Tpst-RC

FBtr0113443

NM_001103503

3344

346

Tpst-RD

FBtr0310432

NM_001272590

4534

346

Tpst-RE

FBtr0310433

NM_001272591

6036

499

Tpst-RF

FBtr0330399

NM_001272589

2734

513

Tpst-RG

FBtr0333723

NM_001272592

4728

346

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tpst-PB

FBpp0290842

58.0

499

7.88

FBpp0112355

39.9

346

8.63

FBpp0302580

39.9

346

8.63

FBpp0302581

58.0

499

7.88

FBpp0303425

59.7

513

8.46

FBpp0305872

39.9

346

8.63

A8JUV2

NP_001259521

AGB95363

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

499 aa isoforms: Tpst-PB, Tpst-PE

346 aa isoforms: Tpst-PC, Tpst-PD, Tpst-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tpst using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.40

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Arbeitman et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in Golgi apparatus

inferred from direct assay

(Bard et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tpst in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tpst

View exonic expression by tissue for Dmel\Tpst

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32312/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086674

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086674

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086674

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32632

Flygut - An atlas of the Drosophila adult midgut

FBgn0086674

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tpst

Transgenic constructs containing regulatory region of Tpst

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6246

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Tpst^GD9110

lethal | pupal stage, with Scer\GAL4^pnr-MD237

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Tpst^GD16237

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Tpst^GD13138

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tpst^GD12703

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Tpst^GD16237

flightless, with Scer\GAL4^Mef2.PR

Tpst^GD13138

some die during pupal stage, with Scer\GAL4^pnr-MD237

Tpst^GD16237

visible, with Scer\GAL4^pnr-MD237

Tpst^GD15801

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

trichogen cell, with Scer\GAL4^pnr-MD237

Tpst^GD15801

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\TPST1

12 of 14

Yes

Hsap\TPST2

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Tpst1

12 of 14

Yes

Rnor\Tpst2

11 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Tpst1

12 of 14

Yes

Mmus\Tpst2

11 of 14

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\tpst2

8 of 13

Yes

Xtro\tpst1

7 of 13

Yes

Xtro\atp1b1

1 of 13

Xtro\polg

1 of 13

Danio rerio (Zebrafish) (4)

Drer\tpst1

12 of 14

Yes

Drer\tpst1l

11 of 14

Yes

Drer\tpst2

5 of 14

Yes

Drer\polr3c

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\tpst-1

12 of 14

Yes

Cele\tpst-2

4 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP000900

10 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tpst. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TPST1; tyrosylprotein sulfotransferase 1

12 of 14

603125

TPST2; tyrosylprotein sulfotransferase 2

11 of 14

603126

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58690

DroID - A comprehensive database of gene and protein interactions.

FBgn0086674

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2016, Mackowiak et al., 2015)

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-44

Cytogenetic map

12A4-12A7

Sequence location

X:13,563,823..13,593,224 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

12A4-12A7

Limits computationally determined from genome sequence between P{EP}CG1640^EP1626 and P{EP}NFAT^EP1353&P{EP}NFAT^EP1508

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-44

(FlyBase, 2016)

1-43.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

11415

w¹¹¹⁸ P{EP}Tpst^EP1218

34418

y¹ w^* Mi{MIC}Tpst^MI01280

97595

y¹ w^* TI{CRIMIC.TG4.2}Tpst^{CR02751-TG4.2}

Kyoto

105392

y^* w^* P{GawB}Tpst^NP7191 / FM7c

1573R-2

1573R-3

15759R-1

15759R-2

v102476

v105756

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (95)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086674

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086674

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified as a candidate gene for hypoxia-specific selection (via an experimental evolution paradigm) that is also differentially expressed between control and hypoxia-adapted larvae.

(Jha et al., 2016)

Annotation (CG32629) eliminated in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Annotation (CG15758) eliminated in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.

(Bard et al., 2006)

dsRNA directed against Tango13 inhibits protein secretion in S2 cells, but has no apparent effect on Golgi organisation.

(Bard et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

New annotation (CG32629) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Tango13 CG15758 CG32629

(FlyBase Genome Annotators, 2007)

Source for merge of: CG1573 CG15759

(FlyBase Genome Annotators, 2002-2003)

Additional comments

One or more of the processed transcripts for these genes contain several non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG46311 (FBgn0284227) and CG32632 (FBgn0086674).

(FlyBase Genome Annotators, 2016)

Annotations CG32632, CG15758 and CG32629 merged as CG32632 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Annotations CG1573 and CG15759 merged as CG32632 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Tango13 CG32632

(Bard et al., 2006)

Source for identity of: Tpst Tango13

(Wang et al., 2018)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG1573

(Arbeitman et al., 2004)

CG15758

CG15759

(Boutros et al., 2004)

CG32629

(Staudt et al., 2005)

CG32629/CG32632

(Staudt et al., 2005)

CG32632

(Hosono et al., 2015, Fujikawa et al., 2009, Keleman et al., 2009.8.5, Haussmann et al., 2008, Bard et al., 2006, Staudt et al., 2005)

DmTPST

(Wang et al., 2018, Huang et al., 2017)

TANGO13

(Bard et al., 2006)

TPST

(Wang et al., 2018, Huang et al., 2017)

Tango13

(Mohr et al., 2017, FlyBase Genome Annotators, 2016, Jha et al., 2016, Mackowiak et al., 2015, Ashwal-Fluss et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Jungreis et al., 2011, Kong et al., 2010, Fujikawa et al., 2009)

Tpst

(Viswanatha et al., 2025, Earl et al., 2020, Gene Disruption Project members, 2018-)

Name Synonyms

CG15758

CG32629

Transport and Golgi organization 13

(Bard et al., 2006)

Transport and golgi organization

(Jha et al., 2016)

Tyrosylprotein sulfotransferase

tyrosylprotein sulfotransferase

(Huang et al., 2017)

Secondary FlyBase IDs

FBgn0040865
FBgn0052632
FBgn0052629
FBgn0030497
FBgn0030498

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 70 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32312

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VYB7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VYB7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VYB7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32312/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086674

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086674

FlyMine - An integrated database for Drosophila genomics

FBgn0086674

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32632

MARRVEL_MODEL - MARRVEL (model organism gene)

32312

Linkouts

BioGRID - A database of protein and genetic interactions.

58690

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086674

DroID - A comprehensive database of gene and protein interactions.

FBgn0086674

DRSC - Results frm RNAi screens

FBgn0086674

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086674

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086674

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32632

Flygut - An atlas of the Drosophila adult midgut

FBgn0086674

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012060

References (54)

The following external sites may use different assemblies or annotations than FlyBase.

-1.40

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