FlyBase Gene Report: Dmel\I-3

General Information

Symbol

Dmel\I-3

Species

D. melanogaster

Name

Inhibitor-3

Annotation Symbol

CG42707

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261624

Gene Model Status

Current

Stock Availability

2 publicly available

Enzyme Name (EC)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

19E4-19E4

Sequence location

X:20,881,814..20,882,626 [-]

Recombination map

1-65

RefSeq locus

NC_004354 REGION:20881814..20882626

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein phosphatase 1 binding

inferred from physical interaction with FLYBASE:flw; FB:FBgn0000711

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1-13C; FB:FBgn0003132

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1α-96A; FB:FBgn0003134

(Bennett et al., 2006)

inferred from physical interaction with FLYBASE:Pp1-87B; FB:FBgn0004103

(Bennett et al., 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables protein phosphatase 1 binding

inferred from biological aspect of ancestor with PANTHER:PTN000464544

(GO Reference Genome Project, 2011-)

enables protein serine/threonine phosphatase inhibitor activity

inferred from biological aspect of ancestor with PANTHER:PTN000464544

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR011107

(InterPro Project Members, 2004-)

enables ubiquitin protein ligase activity

inferred from sequence or structural similarity with UniProtKB:O60927

(Gene Ontology Curators, 2002-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in ubiquitin-dependent protein catabolic process

inferred from sequence or structural similarity with UniProtKB:O60927

(Gene Ontology Curators, 2002-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000464544

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED E3 UBIQUITIN LIGASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Type 1 protein phosphatase inhibitor

Catalytic Activity (EC/Rhea)

ubiquitin protein ligase activity

Summaries

Gene Group (FlyBase)

UNCLASSIFIED E3 UBIQUITIN LIGASES -: This group comprises E3 ubiquitin ligases that do not classify under other groups in FlyBase.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(1)19Ec

Mutant embryos from heterozygous females exhibit no defects in central or peripheral nervous systems; l(1)19Ec1 and l(1)19Ec18 are lethal in germ-line clones, whereas l(1)19Ec8 and l(1)19Ec25 germ-line clones exhibit a paternally rescuable maternal effect. Embryonic phenotype includes head and dorsal-closure defects, and most embryos are twisted (Perrimon, Smouse, and Miklos, 1989, Genetics 121: 313-31).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\I-3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VRC5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.45

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

I-3-RB

FBtr0302971

NM_167716

813

215

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

I-3-PB

FBpp0292096

24.5

215

7.33

Q9VRC5

NP_728367

AAF50877

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Type 1 protein phosphatase inhibitor

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VRC5

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\I-3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

I-3_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.24

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\I-3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\I-3

View exonic expression by tissue for Dmel\I-3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261624

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33068/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261624

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261624

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of I-3

Transgenic constructs containing regulatory region of I-3

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 20 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

I-3^GD7242

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

I-3^GD7242

viable, with Scer\GAL4^Mef2.PR

I-3^GD7242

viable, with Scer\GAL4^tin.CΔ4

I-3^GD7242

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

I-3^GD7242

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\PPP1R11

11 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Ppp1r11

11 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Ppp1r11

10 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\ppp1r11

7 of 13

Yes

Danio rerio (Zebrafish) (1)

Drer\ppp1r11

11 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\C07H6.2

9 of 14

Yes

Cele\K10H10.7

8 of 14

Cele\ZK945.8

6 of 14

Cele\T01D1.3

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (3)

Atha\INH3

7 of 13

Yes

Atha\CYP81D3

1 of 13

Atha\CYP81G1

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YPI1

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC6B12.13

7 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:I-3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG13994

11 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPP1R11; protein phosphatase 1 regulatory inhibitor subunit 11

11 of 14

606670

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

I-3 - Pp1-13C

two hybrid

(Bennett et al., 2006)

I-3 - Pp1-87B

two hybrid

(Bennett et al., 2006)

I-3 - Pp1α-96A

two hybrid

(Bennett et al., 2006)

I-3 - flw

two hybrid

(Bennett et al., 2006)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261624

MIST (protein-protein) - An integrated Molecular Interaction Database

33068

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-65

Cytogenetic map

19E4-19E4

Sequence location

X:20,881,814..20,882,626 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19E4-19E4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-65

(FlyBase, 2016)

1-65.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

VDRC

v108194

P{KK105777}VIE-260B

v45386

w¹¹¹⁸; P{GD7242}v45386

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

AT30947

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261624

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GH22437

Other clones

bs69g12

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261624

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

The "l(1)19Ec" complementation group was previously associated with the CG11233 annotation in FlyBase, based on information in FBrf0155430. However, data from FBrf0193836 suggests that the "l(1)19Ec" complementation group in fact corresponds to the "CG12092" annotation. Thus, the CG11233 annotation has been split out into a separate gene report in FlyBase and the l(1)19Ec complementation group has instead been merged with the gene corresponding to the "CG12092" annotation (Npc1b). The CG11233 annotation has also been renamed to CG42707 in release 5.26 of the genome annotation to avoid confusion.

(FlyBase Genome Annotators, 2010)

FlyBase curator comment: The "l(1)19Ec" complementation group is stated to correspond to the "CG11233" annotation in FBrf0155430. However, data from FBrf0193836 suggests that the "l(1)19Ec" complementation group in fact corresponds to the "CG12092" annotation.

(Bhattacharya, 2003.2.12)

Nomenclature History

Source for database identify of

Source for identity of: I-3 CG42707

(Bennett et al., 2006)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG11233

(Blanco et al., 2010, Keleman et al., 2009.8.5, Bennett et al., 2006, Bhattacharya, 2003.2.12)

CG42707

I-3

(Bennett et al., 2006)

Name Synonyms

Inhibitor-3

(Bennett et al., 2006)

Secondary FlyBase IDs

FBgn0001560

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 21 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33068

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF50877

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VRC5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VRC5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VRC5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261624

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33068/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261624

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261624

InterPro - A database of protein families, domains and functional sites

Type 1 protein phosphatase inhibitor

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42707

MARRVEL_MODEL - MARRVEL (model organism gene)

33068

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261624

DroID - A comprehensive database of gene and protein interactions.

FBgn0261624

DRSC - Results frm RNAi screens

FBgn0261624

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

I-3_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0261624

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013677

MIST (protein-protein) - An integrated Molecular Interaction Database

33068

References (21)

The following external sites may use different assemblies or annotations than FlyBase.

4.24

Report Sections