FlyBase Gene Report: Dmel\fok

General Information

Symbol

Dmel\fok

Species

D. melanogaster

Name

fledgling of Klp38B

Annotation Symbol

CG43690

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263773

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

38B.11, noncoding_3938

Key Links

Genomic Location

Cytogenetic map

38B4-38B4

Sequence location

2L:20,079,270..20,085,070 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:20079270..20085070

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\fok for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8MYU0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Gene model includes transcripts encoding non-overlapping portions of the full CDS.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

fok-RA

FBtr0310558

NM_080713

1205

129

fok-RC

FBtr0310559

NM_001201926

1903

129

fok-RD

FBtr0310560

NM_001273688

696

103

fok-RE

FBtr0310561

NM_001201925

394

Additional Transcript Data and Comments

Reported size (kB)

1.8 (northern blot)

(Molina et al., 1997)

1.2 (longest cDNA)

(Ohkura et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

fok-PA

FBpp0302695

15.3

129

7.04

FBpp0302696

15.3

129

7.04

FBpp0302697

11.7

103

9.27

FBpp0302698

7.9

11.54

M9MRX2

NP_001188854

ADV37103

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

129 aa isoforms: fok-PA, fok-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fok using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fok_1
fok_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.70

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

head mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

Malpighian tubule primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

procrystal cell

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

amnioserosa

(Fisher et al., 2012)

embryonic/larval crystal cell

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & adult stage

(Ohkura et al., 1997)

embryonic stage 9 -- 17

Comment: reference states >=4 hr AEL

(Molina et al., 1997)

adult stage

(Molina et al., 1997)

Additional Descriptive Data

The fok transcript is abundant in 4-8 hr embryos, and is also found in later embyros and in adults.

(Molina et al., 1997)

fok is detected in embryos and adults. Much higher levels of fok transcript are detected in 0-24 hr embryos compared to 0-4 hr embryos.

(Ohkura et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\fok in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\fok

View exonic expression by tissue for Dmel\fok

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263773

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44380/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263773

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020303

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263773

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10746

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of fok

Transgenic constructs containing regulatory region of fok

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

fok^k07614

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fok^GD6652

viable, with Scer\GAL4^Mef2.PR

fok^GD6652

viable, with Scer\GAL4^pnr-MD237

fok^GD6652

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

fok^GD6652

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP008036

7 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:fok. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263773

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

(FlyBase Genome Annotators, 2010)

protein_coding_gene

(FlyBase Genome Annotators, 2010)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

38B4-38B4

Sequence location

2L:20,079,270..20,085,070 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38B4-38B4

Limits computationally determined from genome sequence between P{lacW}barr^k14014 and P{EP}neb^EP642

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

38B4-38B6

(Butler, 2001)

38B-38B

(Ohkura, 1997.1.21)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-53.9

(Comeron et al., 2012)

(Ohkura, 1997.1.21)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

63980

y¹ v¹; P{TRiP.HMJ30293}attP40

83090

y¹ sc^* v¹ sev²¹; P{WKO.1-G8}attP40

34299

y¹ w^*; Mi{MIC}fok^MI02225 neb^MI02225/CyO

55493

y¹ w^*; Mi{MIC}fok^MI10499 neb^MI10499/SM6a

Korea

13688

P{VT010279-GAL4}attP2

11062

P{EP}neb^G8248

11520

P{EP}neb^G12440

14100

P{VT010279-lexA::GADfl}attP40

NIG-Fly

HMJ30293

y¹ v¹; P{TRiP.HMJ30293}attP40/CyO

2LG-3241

y² VhaAC39-1^cho-1 v¹; P{U6-fok.NIG.sgRNA}attP40/CyO

VDRC

v105596

P{KK105919}VIE-260B

v45005

w¹¹¹⁸; P{GD6652}v45005

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH60845

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263773

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG42849) in release 5.30 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Transcription unit identified during molecular analysis of the neb genomic region.

(Ohkura et al., 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: fok CG10746

(Bayraktaroglu, 2000.11.2)

Source for merge of: fok CG42849

(FlyBase Genome Annotators, 2012)

Additional comments

Annotations CG10746 and CG42849 merged as CG43690 in release 5.43 of the genome annotation.

(FlyBase Genome Annotators, 2012)

Merge supported by cDNA, RNA-seq junction, and RNA-seq expression data.

(FlyBase Genome Annotators, 2012)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

38B.11

(Butler, 2001, Butler et al., 2001, McGill Drosophila Genome Project, 2001.9.25)

CG10746

(Hosono et al., 2015, Keleman et al., 2009.8.5, de Velasco et al., 2006, Mack et al., 2006, Butler, 2001, Butler et al., 2001)

CG42849

(FlyBase Genome Annotators, 2010)

CG43690

(National Institute of Genetics Fly Stocks, 2022-, FlyBase Genome Annotators, 2012, Perkins et al., 2009)

Fok

(Cattenoz et al., 2021, Wani et al., 2016, Ruden et al., 1997)

fok

(Hultmark and Andó, 2022, Anqueira-González et al., 2021, Cho et al., 2020, Meltzer et al., 2019, Levis, 2018.8.30, Gene Disruption Project members, 2015-, FlyBase Genome Annotators, 2012, Blanco et al., 2010, Frise et al., 2010, Akdemir et al., 2007, Buszczak et al., 2007, Ni et al., 2006, Domazet-Loso and Tautz, 2003)

l(2)k07614

noncoding_3938

(FlyBase Genome Annotators, 2011, The modENCODE Consortium, 2010)

Name Synonyms

fledgling of Klp38B

(Ohkura et al., 1997)

Secondary FlyBase IDs

FBgn0020303
FBgn0022079
FBgn0032844
FBgn0262096

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44380

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8MYU0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8MYU0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263773

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44380/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263773

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263773

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43690

MARRVEL_MODEL - MARRVEL (model organism gene)

44380

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263773

DroID - A comprehensive database of gene and protein interactions.

FBgn0263773

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fok_1
fok_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020303

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263773

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10746

References (63)

The following external sites may use different assemblies or annotations than FlyBase.

-0.70

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