FlyBase Gene Report: Dmel\CG44774

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG44774 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.99

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

trunk mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

ventral nerve cord primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

anterior midgut primordium

(Fisher et al., 2012)

pars intercerebralis primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

microinjection

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

imaginal disc

Comment: library contains gonads

(Wen et al., 2015)

adult stage | male

testis

(Wen et al., 2015)

Additional Descriptive Data

Tissue-specific RNA-seq reveals that hairpin RNA is dominantly expressed in testis and also accumulates in lbraries from mass-isolated imaginal discs containing larval gonads.

(Wen et al., 2015)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{GawB}CG44774^NP5039

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

lobula columnar neuron LC4

(Flybrain Neuron Database et al., 2018.6.28, Otsuna and Ito, 2006)

lobula | restricted

(Otsuna and Ito, 2006)

antennal mechanosensory and motor center A1 projection neuron

(Matsuo et al., 2016)

giant fiber neuron

(Matsuo et al., 2016)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG44774 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG44774

View exonic expression by tissue for Dmel\CG44774

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266000

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31424/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266000

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029738

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266000

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 25 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG44774

Transgenic constructs containing regulatory region of CG44774

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

Df(1)Ptp4E¹

(Jeon et al., 2008)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG44774^GD11209

viable, with Scer\GAL4^Mef2.PR

CG44774^GD11209

viable, with Scer\GAL4^pnr-MD237

CG44774^GD11209

viable, with Scer\GAL4^tin.CΔ4

CG44774^GD11209

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

CG44774^GD11209

visible, with Scer\GAL4^pnr-MD237

CG44774^GD11209

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

CG44774^GD11209

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\SINHCAF

7 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Sinhcaf

7 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Sinhcaf

7 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\LOC100493285

2 of 13

Yes

Xtro\sinhcaf

2 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\sinhcaf

6 of 14

Yes

Drer\sinhcafl

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG44774. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SINHCAF; SIN3-HDAC complex associated factor

7 of 14

615027

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

CG44774 - Dcr-2

anti tag coimmunoprecipitation, primer specific pcr

(Xiong et al., 2013)

CG44774 - SmD1

anti tag coimmunoprecipitation, primer specific pcr

(Xiong et al., 2013)

CG44774 - stau

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pbl

suppressible

CG44774

(Gregory et al., 2007)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266000

MIST (protein-protein) - An integrated Molecular Interaction Database

31424

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-9

Cytogenetic map

4D6-4D7

Sequence location

X:4,926,664..4,938,105 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4D6-4D7

Limits computationally determined from genome sequence between P{EP}CG2930^EP1352 and P{EP}EP425

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-9

(FlyBase, 2016)

1-9.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33587

w^* P{EP}CG44774^G17311

58526

y¹ w^* Mi{MIC}CG44774^MI12489

FlyORF

F000822

M{UAS-CG44774.ORF.3xHA}ZH-86Fb

10835

11161

112230

w^* P{GawB}CG44774^NP0579 / FM7c

113553

w^* P{GawB}CG44774^NP5039 / FM7c

114112

w^* P{GawB}CG44774^NP7163 / FM7c

104831

y^* w^* P{GawB}CG44774^NP5060 / FM7c

112475

y^* w^* P{GawB}CG44774^NP1056 / FM7c

VDRC

v103486

P{KK105754}VIE-260B

v34786

w¹¹¹⁸; P{GD11209}v34786

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (86)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266000

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CR43698) in release 5.43 of the genome annotation.

(FlyBase Genome Annotators, 2012)

New annotation (CR43699) in release 5.43 of the genome annotation.

(FlyBase Genome Annotators, 2012)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG4068 CR43698 CR43699

(FlyBase Genome Annotators, 2013)

Additional comments

Annotations CG4068, CR43698 and CR43699 merged as CG44774 in release 5.51 of the genome annotation; CR43698 and CR43699 correspond to extended 3' UTR sequences. Merge supported by RNA-Seq expression data.

(FlyBase Genome Annotators, 2013)

"esi-2" overlaps with CG4068 and consists of 20 palindromic approximately 260 nucleotide repeats.

(Czech et al., 2008)

esi-2 consists of 20 tandem repeats that partially overlap the 3'UTR of CG4068.

(Okamura et al., 2008)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG4068

(Palu and Thummel, 2016, Hosono et al., 2015, Laver et al., 2013, Schertel et al., 2013, Sen et al., 2013, Xiong et al., 2013, Desalvo et al., 2011, Marques et al., 2010, Hartig et al., 2009, Keleman et al., 2009.8.5, Czech et al., 2008, FlyBase Curators, 2008-, Jeon et al., 2008, Kawamura et al., 2008, Okamura et al., 2008, Gregory et al., 2007, Maines et al., 2007, Quinones-Coello, 2007, Otsuna and Ito, 2006)

CG44774

(Pang et al., 2021, Ninova et al., 2020, Liu et al., 2019, Meltzer et al., 2019, FlyBase Genome Annotators, 2013)

CR43698

(FlyBase Genome Annotators, 2012)

CR43699

(FlyBase Genome Annotators, 2012)

hp-CG4068

(Wen et al., 2015)

Name Synonyms

Secondary FlyBase IDs

FBgn0029738
FBgn0263836
FBgn0263837

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31424

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A8JUX7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A8JUX7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266000

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31424/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266000

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266000

InterPro - A database of protein families, domains and functional sites

SIN3-HDAC complex-associated factor

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG44774

MARRVEL_MODEL - MARRVEL (model organism gene)

31424

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266000

DroID - A comprehensive database of gene and protein interactions.

FBgn0266000

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029738

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266000

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003210

MIST (protein-protein) - An integrated Molecular Interaction Database

31424

References (49)

The following external sites may use different assemblies or annotations than FlyBase.

1.99

Report Sections