FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
heca, unk
FlyBase ID
FBig0000071496
Interaction Network
Interactions Browser links
heca network
unk network
Reported Interactions
FBrf0216604-39.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
unk 

unk

bait

GFP tag

heca 

hdc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0218395-9394.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unk 
unspecified role
unk 
unspecified role
unk 
unspecified role
heca 
unspecified role
heca 
unspecified role
heca 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 95.89480

FBrf0226218-1.coIP.WB
Reference
Avet-Rochex et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
unk 

Unk

bait

GFP tag

heca 

HdcS

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Avet-Rochex et al., 2014)
FBrf0226218-2.coIP.WB
Reference
Avet-Rochex et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
heca 

HdcS

bait

FLAG tag

unk 

Unk

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Avet-Rochex et al., 2014)
FBrf0241196-1.Y2H
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
heca 

Hdc

bait

fused to unspecified DNA-binding domain

heca 
unk 

Unk

prey

fused to unspecified activation domain

unk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
heca 
N-terminal domain
sufficient binding region
aa 1-650

coordinates relative to hdc-PC

unk 
interaction domain
sufficient binding region
aa 2-154aa 94-360

coordinates relative to unk-PA

coordinates relative to unk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from unspecified library).

(Li et al., 2019)

Two-hybrid system

(Li et al., 2019)
FBrf0241196-2.Y2H
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unk 

Unk

bait

fused to unspecified DNA-binding domain

heca 

Hdc

prey

fused to unspecified activation domain

heca 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
heca 
interaction domain
sufficient binding region
aa 421-639

coordinates relative to hdc-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from unspecified library).

(Li et al., 2019)

Two-hybrid system

(Li et al., 2019)
FBrf0241196-3.coIP.WB
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
heca 

Hdc

bait

FLAG tag

heca 
unk 

Unk

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
heca 
N-terminal domain
sufficient binding region
aa 1-650

coordinates relative to hdc-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2019)
FBrf0241196-4.coIP.WB
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
unk 

Unk

bait

V5 tag

heca 

Hdc

prey

FLAG tag

heca 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
heca 
N-terminal domain
sufficient binding region
aa 1-650

coordinates relative to hdc-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2019)
FBrf0245440-1.coIP.WB
Reference
Maierbrugger et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
heca 

Hdc

bait

FLAG tag

unk 

Unk

prey

HA tag

unk 
unk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
unk 
ZF1-3 domain
sufficient binding region
unk 
Zinc finger domain
necessary binding region
aa 1-297

coordinates relative to unk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Maierbrugger et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)