Dredd, Fadd Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Dredd

DREDD

bait

FLAG tag

Dredd

Fadd

dFADD

prey

HA tag

Fadd

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Fadd

death-inducing domain

sufficient binding region

aa 1-141

"N" construct, coordinates relative to Fadd-PA

Dredd

caspase domain

sufficient binding region

aa 239-494

"casp" construct, weak, coordinates relative to Dredd-PA

Dredd

death-inducing domain

sufficient binding region

aa 1-239

"pro" construct, coordinates relative to Dredd-PA

Dredd

death-inducing domain

necessary binding region

aa 172-239

"proΔ1" construct, coordinates relative to Dredd-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu and Yang, 2000)

Fadd interacts with Dredd but not with Dronc.

(Hu and Yang, 2000)

FBrf0129865-2.coIP.WB

Reference

Hu and Yang, 2000

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Fadd

dFADD

bait

FLAG tag

Dredd

DREDD

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Fadd interacts with Dredd strongly and with Dark weakly.

(Hu and Yang, 2000)

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hu and Yang, 2000)

FBrf0139798-4.coIP.WB

Reference

Horng and Medzhitov, 2001

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

Fadd

dFADD

bait

FLAG tag

Dredd

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Horng and Medzhitov, 2001)

FBrf0214801-2.coIP.WB

Reference

Guntermann and Foley, 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Dredd

bait

Myc tag

Fadd

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guntermann and Foley, 2011)

FBrf0214801-5.coIP.WB

Reference

Guntermann and Foley, 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Fadd

bait

HA tag

Dredd

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guntermann and Foley, 2011)

FBrf0218688-4.coIP.WB

Reference

Meinander et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Fadd

bait

HA or Myc tag

Dredd

prey

V5 or HA tag

Dredd

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Dredd

prodomain DED1

sufficient binding region

aa 1-142

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Meinander et al., 2012)

FBrf0218688-5.coIP.WB

Reference

Meinander et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Dredd

bait

HA or V5 tag

Fadd

prey

Myc or HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Meinander et al., 2012)

FBrf0218992-5.coIP.MS

Reference

Fukuyama et al., 2012

Description

physical association

Assay