Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
D33N, Both participants were mutated. Coordinates relative to mod(mdg4)-PT
D33N, H46D. Both participants were mutated. Coordinates relative to mod(mdg4)-PT
H46D. Both participants were mutated. coordinates relative to mod(mdg4)-PT
D33N, R47Q, Both participants were mutated. Coordinates relative to mod(mdg4)-PT
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. Authors fused GAL4 activation domain to either before or behind the protein and state that only the fusion with GAL4 AD fused behind the protein resulted in strong binding.
Interaction in vitro; bait and prey produced as a recombinant fusion protein in bacterial system.
Coordinates relative to mod(mdg4)-PT
Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
mod(mdg4) formed mainly high-order multimers.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
Crosslinked mod(mdg4) formed mainly high-order multimers.
An N-terminally deleted fragment of the BTB domain fails to interact with itself but will interact with the longer, intact BTB domain.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.
mod(mdg4) formed mainly high-order multimers.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. mod(mdg4) isoforms, PA, PB, PH, PJ, PR, PV, PY, PE, PL, PM, PC, interacted with mod(mdg4)-PT in this assay.
mod(mdg4) isoforms, PA, PB, PH, PJ, PR, PV, PY, PE, PL, PM, PC, interacted with mod(mdg4)-PT in this assay.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus system.
uno forms a tetramer and dimer.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control
I71A, F73A; within the context of aa 1-77
I71K, F73K; within the context of aa 1-77
F73A; within the context of aa 1-77
F73P; within the context of aa 1-77
Interaction in vitro; protein produced as a recombinant fusion proteins in bacterial system.
MW values were determined from column calibration with standard proteins in addition to staining. mod(mdg4) forms multimers. Mutations in hydrophobic residues to break H-bonds (proline), increase polarity (lysine) or traditional alanine, all decreased hexamer formation.
I71A, F73A; within the context of aa 1-77
I71K, F73K; within the context of aa 1-77
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
