gw, AGO1 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

N-terminal region

sufficient binding region

aa 1-539

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Behm-Ansmant et al., 2006)

FBrf0194513-2.PD.A

Reference

Behm-Ansmant et al., 2006

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

GST tag

AGO1

prey

[35]S label

AGO1

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

PIWI domain

sufficient binding region

aa 714-984

N-terminal region

sufficient binding region

aa 1-592

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Behm-Ansmant et al., 2006)

gw interacts with AGO1 but not AGO2.

(Behm-Ansmant et al., 2006)

FBrf0204460-1.coIP.WB

Reference

Eulalio et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

HA tag

AGO1

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

central region

mutation disrupting interaction

aa 594-629

F594V, F629V \'F2V2\' mutant, coordinates relative to 984 residue isoform

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2008)

FBrf0207757-1.coIP.WB

Reference

Iwasaki et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

Ago1

bait

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Iwasaki et al., 2009)

FBrf0208025-1.coIP.WB

Reference

Eulalio et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)

RRM domain of gw not necessary for gw-AGO1 interaction.

(Eulalio et al., 2009)

FBrf0212181-2.coIP.WB

Reference

Jäger and Dorner, 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

HA tag

AGO1

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jäger and Dorner, 2010)

FBrf0212185-1.coIP.WB

Reference

Chekulaeva et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

dGW182

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

sufficient binding region

aa 1-605

coordinates relative to gw-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Chekulaeva et al., 2010)

FBrf0212673-2.coIP.WB

Reference

Huntzinger et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

GFP tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huntzinger et al., 2010)

FBrf0215100-4.coIP.WB

Reference

Zekri et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

GW repeats

mutation disrupting interaction

all N-terminal GW repeats mutated to Alanine

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Zekri et al., 2009)

This interaction is enhanced in Not1-depleted cells.

(Zekri et al., 2009)

FBrf0215456-1.coIP.MS

Reference

Miyoshi et al., 2009

Description

physical association

Assay

peptide massfingerprinting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

TAP tag

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215456-11.coIP.WB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

FLAG tag

AGO1

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

Mid domain

mutation disrupting interaction

aa 560-595

F560V, F595V \'F2V2\' mutant, coordinates relative to 950 residue isoform

AGO1

piwi domain

necessary binding region

aa 691-950

coordinates relative to 950 residue isoform

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215456-2.coIP.WB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Glycerol sedimentation of S2 cell lysates indicates that AGO1 interacts with gw and Dcr-1 in mutually exclusive complexes.

(Miyoshi et al., 2009)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215456-3.coIP.WB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

gw coimmunoprecipitates AGO1 but not AGO2, Dcr-1, Dcr-2 or loqs..

(Miyoshi et al., 2009)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215456-4.coIP.WB

Reference

Miyoshi et al., 2009

Description

physical association

Assay

Collection

Source/Stage

ovary / adult stage female

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

gw coimmunoprecipitates AGO1 but not aub or piwi.

(Miyoshi et al., 2009)

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)

FBrf0215487-1.coIP.WB

Reference

Eulalio et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

interaction domain

mutation decreasing interaction

aa 1-36

deletion of aa 1-36, coordinates relative to gw-PA

interaction domain residues 10-12

mutation decreasing interaction

aa 10-12

GWG to AAA, coordinates relative to gw-PA

interaction domain

mutation disrupting interaction

unspecified

all 12 N-terminal GW-repeats substituted with alanines, coordinates relative to gw-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)

FBrf0215487-2.coIP.WB

Reference

Eulalio et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

HA tag

AGO1

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

interaction domain residues 594,629

mutation disrupting interaction

aa 594,629

F594V,F629V, coordinates relative to AGO1-PA

AGO1

interaction domain residues 777

mutation disrupting interaction

aa 777

F777A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 785

mutation disrupting interaction

aa 785

K785A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 707

mutation decreasing interaction

aa 707

R707A, coordinates relative to AGO1-PA

AGO1

interaction domain residues 749

mutation decreasing interaction

aa 749

Y749A, coordinates relative to AGO1-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2009)

FBrf0216898-2.coIP.WB

Reference

Fukaya and Tomari, 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

Ago1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fukaya and Tomari, 2011)

FBrf0220586-5.coIP.WB

Reference

Huntzinger et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

GFP tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO-binding domain and Q-rich region

sufficient binding region

aa 1-713

GW repeats

mutation disrupting interaction

all 12 \'GW\' repeats mutated to Alanine

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huntzinger et al., 2013)

FBrf0221629-6.coIP.WB

Reference

Hur et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

DmAgo1

bait

FLAG tag

AGO1

GW182

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

AGO1

N-terminal domain

necessary binding region

aa 1-538

coordinates relative to AGO1-PB

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Hur et al., 2013)

FBrf0222297-1.coIP.WB

Reference

Chekulaeva et al., 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

dGW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chekulaeva et al., 2011)

FBrf0222534-10.coIP.WB

Reference

Barišić-Jäger et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

HA tag

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)

The interaction of AGO1 with gw is not affected by Not1 knockdown.

(Barišić-Jäger et al., 2013)

FBrf0222534-4.coIP.WB

Reference

Barišić-Jäger et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

AGO1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)

FBrf0222534-5.coIP.WB

Reference

Barišić-Jäger et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

Twin-Strep tag

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Barišić-Jäger et al., 2013)

The interaction of gw with AGO1 is resistant to RNaseA treatment.

(Barišić-Jäger et al., 2013)

FBrf0222534-8.coIP.WB

Reference

Barišić-Jäger et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

GW182

bait

TAP tag

AGO1

prey

Twin-Strep tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Barišić-Jäger et al., 2013)

Sequential affinity purification suggests that gw, AGO1 and Patr-1 exist in complex.

(Barišić-Jäger et al., 2013)

The interaction of gw with AGO1 is resistant to RNaseA treatment.

(Barišić-Jäger et al., 2013)

Purified AGO1 complexes were subjected to further purification of tagged gw protein.

(Barišić-Jäger et al., 2013)

FBrf0222981-3.coIP.WB

Reference

Wu et al., 2013

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

AGO1

bait

GW182

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wu et al., 2013)

Positive control.

(Wu et al., 2013)

FBrf0232506-1.coIP.WB

Reference

Kuzuoğlu-Öztürk et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used