FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pi3K92E, Pi3K21B
FlyBase ID
FBig0000098367
Interaction Network
Interactions Browser links
Pi3K92E network
Pi3K21B network
Reported Interactions
FBrf0093782-1.coIP.WB
Reference
Weinkove et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

p60

bait
Pi3K92E 

Dp110

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Weinkove et al., 1997)
FBrf0093782-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pi3K92E 

Dp110

enzyme
Pi3K92E 

Dp110

enzyme
Pi3K21B 

p60

enzyme target
Pi3K21B 

p60

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0187416-3.PD.WB
Reference
Wittwer et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

P60

bait

GST tag

Pi3K92E 

dP110

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wittwer et al., 2005)

Pi3K21B binds to both B4 and Pi3K92E in a non-competitive manner. B4 and Pi3K92E do not bind each other.

(Wittwer et al., 2005)
FBrf0193707-2.coIP.WB
Reference
Orme et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Pi3K92E 

Dp110

bait

Myc tag

Pi3K21B 

p60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Orme et al., 2006)
FBrf0216604-62.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Pi3K92E 

Pi3K92E

bait

GFP tag

Pi3K21B 

Pi3K21B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0216604-63.coIP.MS
Reference
Glatter et al., 2011
Description
physical association
Assay
Collection
PPI_InR-Tor
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

Pi3K21B

bait

GFP tag

Pi3K92E 

Pi3K92E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

SAINT score = 1

(Glatter et al., 2011)

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Glatter et al., 2011)
FBrf0230883-2.coIP.MS
Reference
Breitkopf et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

Pi3K21B

bait

TAP tag

Pi3K92E 

Pi3K92E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0230883-5.coIP.MS
Reference
Breitkopf et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pi3K92E 

Pi3k92E

bait

TAP tag

Pi3K21B 

Pi3k21B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0233454-763.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

Pi3K21B

bait

TAP tag

Pi3K92E 

Pi3K92E

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

(Vinayagam et al., 2016)
FBrf0233454-810.coIP.MS
Reference
Vinayagam et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pi3K92E 

Pi3K92E

bait

TAP tag

Pi3K21B 

Pi3K21B

prey

SAINT score = 1

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Vinayagam et al., 2016)

Observed in all three conditions tested: uninduced cells, 10 minutes after insulin stimulation and 30 minutes after insulin stimulation.

(Vinayagam et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)