FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cp190, Map60
FlyBase ID
FBig0000102412
Interaction Network
Interactions Browser links
Cp190 network
Map60 network
Reported Interactions
FBrf0057159-1.coIP.WB
Reference
Kellogg and Alberts, 1992
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait
Map60 

CP60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kellogg and Alberts, 1992)
FBrf0058813-1.coIP.WB
Reference
Raff et al., 1993
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

DMAP190

bait
Map60 

DMAP60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Raff et al., 1993)
FBrf0085334-1.coIP.WB
Reference
Kellogg et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Map60 

CP60

bait
Cp190 

CP190

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kellogg et al., 1995)
FBrf0085334-2.coIP.MW
Reference
Kellogg et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait
Map60 

CP60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kellogg et al., 1995)
FBrf0224437-1.coIP.MS
Reference
Cuartero et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

TAP tag

Map60 

CP60

prey

17 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Cuartero et al., 2014)

TAP tandem affinity purification of bait and identification of prey by mass spectrometry.

(Cuartero et al., 2014)
FBrf0227255-1.coIP.MS
Reference
Maksimenko et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

FLAG tag

Map60 

Map60

prey

26 unique peptides

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Map60 enriched 20-fold in Cp190 purification compared to negative control.

(Maksimenko et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Maksimenko et al., 2015)
FBrf0253430-32.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

Cp190

bait

GFP tag

Map60 

Cp60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
FBrf0257791-12.Y2H
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait

fused to GAL4 DNA-binding domain

Map60 

CP60

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2023)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Melnikova et al., 2023)
FBrf0257791-16.Y2H
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Map60 

CP60

bait

fused to GAL4 DNA-binding domain

Map60 
Cp190 

CP190

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Map60 
interaction domain
sufficient binding region
aa 104-189

coordinates relative to Map60-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Melnikova et al., 2023)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Melnikova et al., 2023)
FBrf0257791-19.EMSA
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Map60 

CP60

neutral component

His tag

Map60 

CP60

neutral component

His tag

Map60 

CP60

neutral component

His tag

Cp190 

CP190

neutral component

His tag

Cp190 

CP190

neutral component

His tag

Cp190 

CP190

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Map60, Cp190 and su(Hw) was mixed with Cy5 labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

(Melnikova et al., 2023)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Melnikova et al., 2023)

Map60 interacts with the Cp190-su(Hw) insulator complex via Cp190.

(Melnikova et al., 2023)
FBrf0257791-2.coIP.WB
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Map60 

CP60

bait
Cp190 

CP190

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of S2 cell line; bait and prey produced from endogenous gene.

(Melnikova et al., 2023)
FBrf0257791-5.coIP.WB
Reference
Melnikova et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cp190 

CP190

bait
Map60 

CP60

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of S2 cell line; bait and prey produced from endogenous gene.

(Melnikova et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)