Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The nos-Not1 interaction is RNA-independent.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

nos binds only preassembled Not1-Rga-Not3 complex, and does not bind any single Not subunit individually.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Positive control.

nanos was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.

Source was live S2R+ cells; bait and prey produced from endogenous genes.