Dlp
V5 tag or untagged
Wg
GFP tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
wg can interact with the nonglycanated form of dlp but the interaction is stronger with the glycanated form.
SVS tag
HA tag
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.
biotin tag
V5 tag
coordinates relative to dlp-PA
Interaction in vitro; bait was synthetic peptide; prey derived from conditioned medium of transfected S2 cells.
dlp preferentially binds a palmitoleoylated wg peptide.
