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Vyas, N., Goswami, D., Manonmani, A., Sharma, P., Ranganath, H.A., VijayRaghavan, K., Shashidhara, L.S., Sowdhamini, R., Mayor, S. (2008). Nanoscale organization of hedgehog is essential for long-range signaling.  Cell 133(7): 1214--1227.
FlyBase ID
FBrf0215626
Publication Type
Research paper
Abstract

Hedgehog (Hh) plays crucial roles in tissue-patterning and activates signaling in Patched (Ptc)-expressing cells. Paracrine signaling requires release and transport over many cell diameters away by a process that requires interaction with heparan sulfate proteoglycans (HSPGs). Here, we examine the organization of functional, fluorescently tagged variants in living cells by using optical imaging, FRET microscopy, and mutational studies guided by bioinformatics prediction. We find that cell-surface Hh forms suboptical oligomers, further concentrated in visible clusters colocalized with HSPGs. Mutation of a conserved Lys in a predicted Hh-protomer interaction interface results in an autocrine signaling-competent Hh isoform--incapable of forming dense nanoscale oligomers, interacting with HSPGs, or paracrine signaling. Thus, Hh exhibits a hierarchical organization from the nanoscale to visible clusters with distinct functions.

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Note

Hedgehog nanopackages ready for dispatch.
Vincent, 2008, Cell 133(7): 1139--1141 [FBrf0215781]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (10)
    Genes (9)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (4)
    Transgenic Constructs (7)