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General Information
Symbol
P{BS3.0}
FlyBase ID
FBtp0000694
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
dpp-LacZ, dpp-lacZ BS3.0, dpp-lacZBS3.0, BS3.0, dppZ, dpp-lacZ (BS3.0), dpp:lacZ
Size
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Marker(s)
Component allele Ecol\lacZdpp.BS3.0
Product class / Tool use(s)
Encoded product / tool
Description

lacZ expression is driven by fragment from 106.9-116.9kb of dpp, at the disk III-disk II boundary.

12kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

10kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

A dpp disc enhancer drives lacZ expression.

lacZ expression is controlled by the 3' dpp enhancer.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
chimeric
gene
reporter
gene
selectable
marker
Component Alleles
Reference(s)
(Raj et al., 2020, Spannl et al., 2020, Tare et al., 2020, Romero-Pozuelo et al., 2019, Tanaka-Matakatsu et al., 2015, Tsai et al., 2015, Bilak et al., 2014, Herranz et al., 2014, Tanaka-Matakatsu et al., 2014, Chauhan et al., 2013, Fan et al., 2013, Li et al., 2013, Yang et al., 2013, Zhang et al., 2013, Zhang et al., 2013, Zhang et al., 2013, Fan et al., 2012, Legent et al., 2012, Marchal et al., 2012, Mukherjee et al., 2012, Wang and Sun, 2012, Du et al., 2011, Shi et al., 2011, Yang and Su, 2011, Zhang et al., 2011, Jia et al., 2010, Seong et al., 2010, Usha and Shashidhara, 2010, Zheng et al., 2010, Jia et al., 2009, Khaliullina et al., 2009, Svendsen et al., 2009, Zhang et al., 2009, Chang et al., 2008, Kennell et al., 2008, Liu et al., 2008, Miura et al., 2008, Vyas et al., 2008, Kugler and Nagel, 2007, Malpel et al., 2007, Pfleger et al., 2007, Tanaka-Matakatsu et al., 2007, Theisen et al., 2007, Zhao et al., 2007, Bras-Pereira et al., 2006, Callejo et al., 2006, de Navas et al., 2006, Dussillol-Godar et al., 2006, Ekas et al., 2006, Hennig et al., 2006, Kent et al., 2006, Lee et al., 2006, Ogden et al., 2006, Stultz et al., 2006, Vrailas and Moses, 2006, Yao et al., 2006, Zhang et al., 2006, Collins and Cohen, 2005, Garoia et al., 2005, Glise et al., 2005, Gorfinkiel et al., 2005, Ho et al., 2005, Horabin, 2005, Jia et al., 2005, Joulia et al., 2005, Katsuyama et al., 2005, Kenyon et al., 2005, Pimentel and Venkatesh, 2005, Rogers et al., 2005, Takahashi et al., 2005, Takeo et al., 2005, Thompson et al., 2005, Zhang et al., 2005, Collier et al., 2004, del Alamo Rodriguez et al., 2004, Janody et al., 2004, Jia et al., 2004, Marenda et al., 2004, Nakano et al., 2004, Niwa et al., 2004, Perez-Garijo et al., 2004, Tavsanli et al., 2004, Torroja et al., 2004, Wang and Struhl, 2004, Zeng and Verheyen, 2004, Dai et al., 2003, Estella et al., 2003, Ghazi et al., 2003, Gorfinkiel et al., 2003, Jang et al., 2003, Jia et al., 2003, Johnson et al., 2003, Kango-Singh et al., 2003, Kenyon et al., 2003, Lin et al., 2003, Lukacsovich et al., 2003, Sedkov et al., 2003, Belenkaya et al., 2002, Bessa et al., 2002, Cadigan et al., 2002, Cavodeassi et al., 2002, Chanut et al., 2002, Chern and Choi, 2002, Erkner et al., 2002, Ghbeish and McKeown, 2002, Jia et al., 2002, Lee et al., 2002, Noureddine et al., 2002, Ou et al., 2002, Parker et al., 2002, Pickup et al., 2002, Abzhanov et al., 2001, Amanai and Jiang, 2001, Apidianakis et al., 2001, Estrada and Sanchez-Herrero, 2001, Kumar and Moses, 2001, Lee and Treisman, 2001, Lee and Treisman, 2001, Lee et al., 2001, Mozer, 2001, Sanchez et al., 2001, Treisman, 2001, Aza-Blanc et al., 2000, Azpiazu and Morata, 2000, Baonza et al., 2000, Benlali et al., 2000, Calleja et al., 2000, Certel et al., 2000, Chanut et al., 2000, Cho et al., 2000, Collins and Treisman, 2000, Entchev et al., 2000, Garoia et al., 2000, Haines and van Den Heuvel, 2000, Lai et al., 2000, Maurel-Zaffran and Treisman, 2000, McCartney et al., 2000, Miletich and Limbourg-Bouchon, 2000, Mohler et al., 2000, Mukherjee et al., 2000, Niwa et al., 2000, Pichaud and Casares, 2000, Randsholt et al., 2000, Song et al., 2000, Svendsen et al., 2000, Teleman and Cohen, 2000, Zeng et al., 2000, Asano and Wharton, 1999, Chen et al., 1999, Gorfinkiel et al., 1999, Goto and Hayashi, 1999, Greenwood and Struhl, 1999, Heanue et al., 1999, Kopp et al., 1999, Mandal and Lakhotia, 1999, Methot and Basler, 1999, Moreno and Morata, 1999, Vervoort et al., 1999, Wang et al., 1999, Weigmann and Cohen, 1999, Yao et al., 1999, Abu-Shaar and Mann, 1998, Alves et al., 1998, Borod and Heberlein, 1998, Emerald and Roy, 1998, Go et al., 1998, Hazelett et al., 1998, Heberlein et al., 1998, Jiang and Struhl, 1998, Khalsa et al., 1998, Maschat et al., 1998, Maves and Schubiger, 1998, Saget et al., 1998, Theodosiou et al., 1998, Weatherbee et al., 1998, Buratovich and Bryant, 1997, Casares et al., 1997, Chanut and Heberlein, 1997, Chanut and Heberlein, 1997, Chen and Baker, 1997, Chen et al., 1997, Dominguez and Hafen, 1997, Epps et al., 1997, Gorfinkiel et al., 1997, Heslip et al., 1997, Jackson et al., 1997, Lecuit and Cohen, 1997, Mullor et al., 1997, Nestoras et al., 1997, Newfeld et al., 1997, Penton et al., 1997, Pignoni and Zipursky, 1997, Pignoni et al., 1997, Royet and Finkelstein, 1997, Sanchez et al., 1997, Schneider and Spradling, 1997, Shen and Mardon, 1997, Strigini and Cohen, 1997, Strutt and Mlodzik, 1997, Sturtevant et al., 1997, Treisman et al., 1997, Weigmann et al., 1997, Zelhof et al., 1997, Adamson and Shearn, 1996, Alexandre et al., 1996, Brook and Cohen, 1996, Burke and Basler, 1996, Chen and Struhl, 1996, Freeland and Kuhn, 1996, Johnston and Schubiger, 1996, Lecuit et al., 1996, Morimura et al., 1996, Nellen et al., 1996, Sanchez-Herrero et al., 1996, Strutt and Mlodzik, 1996, van den Heuvel and Ingham, 1996, Buratovich and Bryant, 1995, Chanut and Heberlein, 1995, Curtiss and Heilig, 1995, Felsenfeld and Kennison, 1995, Guillen et al., 1995, Heberlein et al., 1995, Ingham and Fietz, 1995, Jarman et al., 1995, Jiang and Struhl, 1995, Lepage et al., 1995, Li et al., 1995, Ma and Moses, 1995, Martin et al., 1995, Pan and Rubin, 1995, Sanicola et al., 1995, Simmonds et al., 1995, Strutt and Mlodzik, 1995, Strutt et al., 1995, Treisman and Rubin, 1995, Treisman et al., 1995, Capdevila and Guerrero, 1994, Capdevila et al., 1994, Kojima et al., 1994, Lee et al., 1994, Heberlein et al., 1993, Ma et al., 1993, Smith et al., 1993, Blair, 1992, Blackman et al., 1991, Raftery et al., 1991)
Molecular data

lacZ expression is controlled by the 3' dpp enhancer.

A dpp disc enhancer drives lacZ expression.

10kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

12kb EcoRI fragment spanning dpp region disk III--II boundary drives lacZ expression.

lacZ expression is driven by fragment from 106.9-116.9kb of dpp, at the disk III-disk II boundary.

Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

The dpp reporter is mostly expressed anterior to phosphorylated Mad at the morphogenetic furrow in third instar larval eye discs.

Expression is observed in both the male and female genital discs, and is complementary to that of wg. Expression is excluded from the repressed male primordium in the female genital disc.

The Ecol\lacZdpp.BS3.0 reporter construct reflects wildtype expression of dpp in the imaginal discs. Expression is detected in a stripe along the anterior-posterior boundary of the wing disc and along the morphogenetic furrow in the eye-antennal disc.

Expressed in cells on anterior side of the A/P boundary.

The expression pattern of Ecol\lacZdpp.BS3.0 most closely recapitulates wild-type dpp expression patterns during development.

 
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: One of a series of lacZ reporter constructs used to characterize the disk 3\' regulatory region of dpp.

Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (42)
Reported As
Symbol Synonym
(Dpp)-lacZ line 3.0
BS3.0 decapentaplegic
BS3.0 dpp LacZ
BS3.0dpp-lacZ BS3.0
P[dpp-lacZ]
P{dpp-lacZ}dpp-lacZ
dpp-LacZ BS3.0
dpp-LacZ<up>BS 3.0</up>
dpp-lacZ(BS3.0)
dpp-lacZdpp.BS3.0
dpp-lacZdpp.BS3.0RA
dpp-lacZBS3.0
dpp-lacZ{BS3.0}
dppBS3.0-lacZ
dppBS3.0:LacZ
Secondary FlyBase IDs
    References (251)