Chen, P.H., Lee, C.I., Weng, Y.T., Tarn, W.Y., Tsao, Y.P., Kuo, P.C., Hsu, P.H., Huang, C.W., Huang, C.S., Lee, H.H., Wu, J.T., Chen, S.L. (2013). BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. RNA 19(2): 208--218.
FlyBase ID
FBrf0220579
Publication Type
Research paper
Abstract
Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.
