Link, N., Kurtz, P., O'Neal, M., Garcia-Hughes, G., Abrams, J.M. (2013). A p53 enhancer region regulates target genes through chromatin conformations in cis and in trans. Genes Dev. 27(22): 2433--2438.
FlyBase ID
FBrf0223347
Publication Type
Research paper
Abstract
We examined how a p53 enhancer transmits regulatory information in vivo. Using genetic ablation together with digital chromosome conformation capture and fluorescent in situ hybridization, we found that a Drosophila p53 enhancer region (referred to as the p53 response element [p53RE]) physically contacts targets in cis and across the centromere to control stress-responsive transcription at these sites. Furthermore, when placed at ectopic genomic positions, fragments spanning this element re-established chromatin contacts and partially restored target gene regulation to mutants lacking the native p53RE. Therefore, a defined p53 enhancer region is sufficient for long-range chromatin interactions that enable multigenic regulation.
