Long, X., Colonell, J., Wong, A.M., Singer, R.H., Lionnet, T. (2017). Quantitative mRNA imaging throughout the entire Drosophila brain. Nat. Methods 14(7): 703--706.
FlyBase ID
FBrf0235908
Publication Type
Research paper
Abstract
We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).
