The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Xiangzhong Zheng, Indiana University.
P{UAS-foxo.DeltaC.V5} The coding sequence of foxo lacking the C-terminal activation domain was fused in frame with V5 tag and inserted into the pP{UAST} vector. It expresses a dominant negative FOXO that binds to target DNA sequences but incapable of transcriptional activation of target genes under the control of UAS sequences.
P{UAS-foxo.dsRNA} An inverted repeat of foxo coding sequences (~500 bp) was inserted into the transformation vector pP{UAST}. It expresses a dsRNA against foxo under the control of UAS sequences.
P{foxo-GAL4} A 5000 bp promoter fragment of foxo was inserted into the transformation vector pP{PTGAL}. It expresses GAL4 under the control of foxo promoter sequences.
P{hs-HA.smt3} The coding sequence of smt3 with a HA tag at the N-terminus was inserted into the transformation vector pP{CaSpeR-hs}. It expresses HA-tagged SMT3 under the control of the hsp70 promoter sequence.
P{hs-smt3.HA} The coding sequence of smt3 with a HA tag at the C-terminus was inserted into the transformation vector pP{CaSpeR-hs}. It expresses HA-tagged SMT3 under the control of the hsp70 promoter sequence.
P{Cam.CGCGbox-luc2P} An enhancer (~ 125 bp) containing multimeric CGCG Box sequences (A/C/G)CGCG(G/T/C) of the Cam gene and the hsp70 basal promoter was placed upstream of the luciferase reporter gene luc2P and subcloned into the pP{CaSpeR-4} vector. It expresses a destabilized luciferase luc2P under the control of Cam CGCG Box sequences.
P{hs-Rheb} The coding sequence of Rheb was inserted into the transformation vector pP{CaSpeR-hs}. It expresses RHEB under the control of the hsp70 promoter sequence.
P{hs-Pten} The coding sequence of Pten was inserted into the transformation vector pP{CaSpeR-hs}. It expresses PTEN under the control of the hsp70 promoter sequence.
P{hs-AMPKalpha} The coding sequence of AMPKalpha was inserted into the transformation vector pP{CaSpeR-hs}. It expresses AMPKalpha under the control of the hsp70 promoter sequence.
P{UAS-gig.wiz} A fragment of the gig coding sequences (~500 bp) was inserted into the transformation vector pWIZ. It expresses a dsRNA against gig under the control of UAS sequences.
P{UAS-gig.DeltaGAP} The coding sequence of gig lacking the GAP domain was inserted into the transformation vector pP{UAST}. It expresses a dominant negative TSC2 capable of binding to TSC1 but lacks the GAP activity towards RHEB.
P{UAS-Pkc53E.RB} The coding sequence of Pkc53E RB isoform was inserted into the transformation vector pP{UAST}. It expresses PKC53E under the control of UAS sequences.
P{mir-275.mir-305} A genomic DNA fragment (~ 4 kb) containing mir-275 and mir-305 was inserted into the pP{CaSpeR-4} vector. It expresses mir-275 and mir-305 under the control of their own promoter sequence.
P{mir-275-GAL4} A genomic DNA fragment (~ 3.4 kb) containing the mir-275 promoter sequence was inserted into the transformation vector pP{PTGAL}. It expresses GAL4 under the control of mir-275 promoter sequence.
P{per.S820A.S822A} This construct was generated using a similar approach described in Garbe et al. (2013), PLoS Genet. 9(9): e1003749(FBrf0222889). The per promoter and coding sequence with the Ser820 and Ser822 codons mutated to Ala was inserted into the transformation vector pP{CaSpeR-4}. It expresses PER.S820A.S822A under the control of per promoter sequence.
P{per.S809A} This construct was generated using a similar approach described in Garbe et al. (2013), PLoS Genet. 9(9): e1003749(FBrf0222889). The per promoter and coding sequence with the Ser809 codon mutated to Ala was inserted into the transformation vector pP{CaSpeR-4}. It expresses PER.S809A under the control of per promoter sequence.
P{per.S820A} This construct was generated using a similar approach described in Garbe et al. (2013), PLoS Genet. 9(9): e1003749(FBrf0222889). The per promoter and coding sequence with the Ser820 codon mutated to Ala was inserted into the transformation vector pP{CaSpeR-4}. It expresses PER.S820A under the control of per promoter sequence.
P{UAS-Txl.C33S} The coding sequence with the Cys33 codon mutated to Ser was inserted into the transformation vector pP{UAST}. It expresses TXL.C33S under the control of UAS sequences.
P{UAS-Txl.C36S} The coding sequence with the Cys36 codon mutated to Ser was inserted into the transformation vector pP{UAST}. It expresses TXL.C36S under the control of UAS sequences.
P{UAS-Txl.V5} The coding sequence of Txl with the V5 tag in frame at the C-terminus was inserted into the transformation vector pP{UAST}. It expresses V5-tagged TXL under the control of UAS sequences.
P{UAS-Txl} The coding sequence of Txl was inserted into the transformation vector pP{UAST}. It expresses TXL under the control of UAS sequences.
P{UAS-MagR.DM} The coding sequence of MagR from Drosophila melanogaster was inserted into the transformation vector pP{UAST}. It expresses MagR.DM under the control of UAS sequences.
P{UAS-MagR.DP} The coding sequence of MagR from the monarch butterfly (Danaus plexippus) was inserted into the transformation vector pP{UAST}. It expresses MagR.DP under the control of UAS sequences.
P{UAS-foxo.DeltaC.V5}1 is an X chromosome insertion, homozygous viable and fertile.
P{UAS-foxo.DeltaC.V5}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-foxo.dsRNA}3 is a third chromosome insertion, homozygous viable and fertile.
P{foxo-GAL4}9A1 is an X chromosome insertion, homozygous viable and fertile.
P{hs-HA.smt3}2 is a second chromosome insertion, homozygous viable and fertile.
P{hs-HA.smt3}3 is a third chromosome insertion, homozygous viable and fertile.
P{hs-smt3.HA}2 is a second chromosome insertion, homozygous viable and fertile.
P{hs-HA.smt3}1 is an X chromosome insertion, homozygous viable and fertile.
P{Cam.CGCGbox-luc2P}2 is a second chromosome insertion, homozygous viable and fertile.
P{hs-Rheb}9 is a second chromosome insertion, homozygous viable and fertile.
P{hs-Pten}3C1 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-gig.wiz}2C1 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-gig.wiz}3A1 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-gig.DeltaGAP}1-10 is a second chromosome insertion, homozygous viable and fertile.
P{hs-AMPKalpha}2 is a second chromosome insertion, homozygous viable and fertile.
P{hs-AMPKalpha}3 is a third chromosome insertion.
P{UAS-Pkc53E.RB}3 is a third chromosome insertion.
P{mir-275.mir-305}1 is an X chromosome insertion, homozygous viable and fertile.
P{per.S820A.S822A}3 is a third chromosome insertion, homozygous viable and fertile.
P{per.S809A}2 is a second chromosome insertion, homozygous viable and fertile.
P{per.S820A}2A1 is a second chromosome insertion, homozygous viable and fertile.
P{mir-275-GAL4}3 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Txl.C33S}6 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Txl.C36S}2 is a third chromosome insertion.
P{UAS-Txl.C36S}1 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-Txl.C33S}2 is a second chromosome insertion.
P{UAS-Txl.V5}1B1 is an X chromosome insertion, homozygous viable and fertile.
P{UAS-Txl.V5}3B1 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Txl}3A1 is a third chromosome insertion, homozygous viable and fertile.
P{UAS-Txl}2A1 is a second chromosome insertion, homozygous viable and fertile.
P{UAS-MagR.DM}5 is a third chromosome insertion.
P{UAS-MagR.DP}2 is a second chromosome insertion.
P{UAS-MagR.DP}6 is a third chromosome insertion.
TxlDelta255 The coding sequence of Txl was deleted through imprecise excision of P{EPgy2}CG10576EY07625. It is a null allele.
CG12795Delta118 The coding sequence of CG12795 was deleted through imprecise excision of P{EPgy2}CG12795EY03155. It is a null allele. CG12795 is a homolog of aip-1 in C.elegans.
Pkc53EDelta28 The coding sequence of Pkc53E was deleted through imprecise excision of P{EPgy2}Pkc53EEY14093. It is a null allele.