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Citation
Ding, K., Han, Y., Seid, T.W., Buser, C., Karigo, T., Zhang, S., Dickman, D.K., Anderson, D.J. (2019). Imaging neuropeptide release at synapses with a genetically engineered reporter.  eLife 8(): e46421.
FlyBase ID
FBrf0242860
Publication Type
Research paper
Abstract

Research on neuropeptide function has advanced rapidly, yet there is still no spatio-temporally resolved method to measure the release of neuropeptides in vivo. Here we introduce Neuropeptide Release Reporters (NPRRs): novel genetically-encoded sensors with high temporal resolution and genetic specificity. Using the Drosophila larval neuromuscular junction (NMJ) as a model, we provide evidence that NPRRs recapitulate the trafficking and packaging of native neuropeptides, and report stimulation-evoked neuropeptide release events as real-time changes in fluorescence intensity, with sub-second temporal resolution.

PubMed ID
PubMed Central ID
PMC6609332 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference