FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Vieira, R., Borst, A. (2025.5.27). Lines from R. Vieira and A. Borst. 
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FBrf0262482
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The following information accompanied stocks donated to the Bloomington Stock Center by Renee Vieira and Alexander Borst, Max-Planck-Institut fur Biologische Intelligenz, Martinsried.
P{3XUAS-Dre.PEST} was generated as part of the work associated with Nern et al. (2011; FBrf0214794). It is identical to P{20XUAS-Dre.PEST} except that it has three UAS copies instead of twenty. The plasmid was obtained from Janelia Research Campus, and the attB-flanked segment was targeted to the P{CaryIP}su(Hw)attP1 site (87B3,  3R:12474075..12477316  (R6)) to generate P{3XUAS-Dre.PEST}su(Hw)attP1.
P{Hsp70- KD::PEST } was generated from the pJFRC161-20XUAS-IVS- KD::PEST  plasmid (Addgene 32140) generated by Nern et al. (2011; FBrf0214794) by substituting Hsp70 regulatory sequences for UAS sequences.
P{Hsp70- KD::PEST }su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 site at 50F1,  2R:14307368..14307690  (R6).
P{10XUAS(KDRT.stop)FLP} expresses FLP recombinase under the control of ten copies of UAS upon removal of a KDRT-flanked cassette containing a translational stop sequence. It was generated by modifying the pJFRC176-10XUAS-rox>-dSTOP-rox>- myr::GFP  plasmid (Addgene 32147) generated by Nern et al. (2011; FBrf0214794) by substituting KDRT sequences for rox sequences and FLP sequences for myr-tagged GFP sequences.
P{10XUAS(KDRT.stop)FLP}attP40 is an insertion into P{CaryP}Msp300attP40 at 25C6,  2L:5108448  (R6).
P{10XUAS(FRT.stop) myr::tdTomato.V } expresses myr-tagged tdTomato under the control of ten UAS sequences upon removal of an FRT-flanked translational stop cassette. It was generated by modifying the pJFRC177-10XUAS-FRT>-dSTOP-FRT>- myr::GFP  plasmid (Addgene 32149) from Nern et al. (2011; FBrf0214794) by substituting tdTomato sequences for GFP sequences.
P{10XUAS(FRT.stop) myr::tdTomato.V }su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 site at 50F1,  2R:14307368..14307690  (R6).
P{10XUAS(FRT.stop) CD4::tdTomato.V } is identical to P{10XUAS(FRT.stop) myr::tdTomato.V } except for the substitution of CD4 sequences for myr sequences. 
P{10XUAS(FRT.stop) CD4::tdTomato.V }su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at  2R:14 ,307,368..14,307,690 (R6).
P{10XUAS(FRT.stop) CD4::tdTomato.V }su(Hw)attP1 is an insertion into the P{CaryIP}su(Hw)attP1 target site at  3R:12 ,474,075..12,477,316 (R6).
P{10XUAS(D6rox.stop)FLP} was generated by modifying the pJFRC176-10XUAS-rox>-dSTOP-rox>- myr::GFP  plasmid (Addgene 32147) generated by Nern et al. (2011; FBrf0214794) to substitute FLP sequences for GFP sequences.
P{10XUAS(D6rox.stop)FLP}su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at  2R:14 ,307,368..14,307,690 (R6).
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