FlyBase Insertion Report: Dmel\P{lacZ.ry[+]}loco[rC56]

General Information

Symbol

Dmel\P{lacZ.ry⁺}loco^rC56

Species

D. melanogaster

Name

FlyBase ID

FBti0000730

Feature type

transposable_element_insertion_site

Also Known As

rC56, P{lacZ-un1}loco^rC56, loco-lacZ

Description

Inserted Element

P{lacZ.ry⁺}

LINE ID

Affected gene(s)

Ecol\lacZ, loco

Viability / fertility

fertile, viable

Associated allele(s)

Ecol\lacZ^loco-rC56, loco^rC56

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 94B7 )

Sequence Location

3R:22,626,614..22,626,967 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:22,626,614..22,626,967 [-]

(Granderath et al., 1999)

Orientation

(Granderath et al., 1999)

Cytological location (computed by FlyBase)

94B7 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

The P{lacZ-un1}loco^rC56 insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ}loco^3-109.

(Granderath et al., 1999)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacZ.ry⁺}

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Ding et al., 2011, Kaplow et al., 2008, Thomas and van Meyel, 2007, Pielage et al., 2004, Shandala et al., 2003, Yuasa et al., 2003, Granderath et al., 2000, Granderath et al., 1999, Bernardoni et al., 1998, Klambt, 1998.8.10, Kretzschmar et al., 1997, Wemmer and Klämbt, 1995, Menne and Klambt, 1994)

loco

(Granderath et al., 1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^loco-rC56

Phenotypic Class Phenotype Manifest in Detailed Description

loco^rC56

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

CNS glial cell | subset

(Menne and Klambt, 1994)

lateral ectoderm | dorsal

(Granderath et al., 1999)

embryonic stage 12

embryonic longitudinal glial cell

(Klaes et al., 1994)

glial cell of larval VUM neuron

(Klaes et al., 1994)

embryonic stage 12 -- 17

glial cell

(Granderath et al., 1999)

embryonic stage 13 -- 17

segmental nerve root glial cell

(Klaes et al., 1994)

exit glial cell

(Klaes et al., 1994)

larval B-subperineurial glial cell

(Klaes et al., 1994)

larval A-subperineurial glial cell

(Klaes et al., 1994)

medial intersegmental nerve root glial cell

(Klaes et al., 1994)

embryonic stage 16

larval lateral VNC glial cell

(Granderath et al., 2000)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval glial cell

(Kaplow et al., 2008)

larval brain | restricted

(Kaplow et al., 2008)

eye disc

(Kaplow et al., 2008)

Additional Information

Statement

Reference

loco reporter expression is observed in most glial cells of the larval brain including surface, cortex, and neuropile glia. St

(Kaplow et al., 2008)

Enhancer trap expression is detected at embryonic stage 12 in cells which appear to be progeny of the lateral glioblast. Those cells migrate medially and divide. By the end of embryogenesis, enhancer trap expression is observed in most glial cells except the midline glia. Expression is also seen in the dorsal leading edge cells of the lateral ectoderm.

(Granderath et al., 1999)

Marker for

larval lateral VNC glial cell

(Granderath et al., 2000)

Reflects expression of

loco

(Kaplow et al., 2008, Granderath et al., 2000, Granderath et al., 1999)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

10009

P{lacZ.ry⁺}loco^rC56

External Crossreferences and Linkouts ( 0 )

Comments

The P{lacZ-un1}loco^rC56 insertion site maps between an EcoRI site and the loco-RC transcription start and is reported to be within 1 bp of P{lacZ}3-109. It is in a positive orientation with respect to loco gene transcription.

(Granderath et al., 1999)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

P{A92}rC56

(FlyBase, 1992-)

P{PZ}rC56

(FlyBase, 1992-)

P{lacZ-un1}loco^rC56

(Kaplow et al., 2008, Granderath et al., 1999, FlyBase, 1992-)

P{lacZ-un1}rC56

(Menne and Klambt, 1994, FlyBase, 1992-)

P{lacZ.ry⁺}loco^rC56

P{lacZ}rC56

(FlyBase, 1992-)

P{ry^+t7.2=PZ}rC56

(FlyBase, 1992-)

loco-lacZ

(Ding et al., 2011, Thomas and van Meyel, 2007)

rC56

(Pielage et al., 2004, Klaes et al., 1994)

rc56

(Shandala et al., 2003, Granderath et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBti0002154
FBti0009198

References (17)

Report Sections

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