FB2026_03 , released September 17, 2026
Insertion: Dmel\P{lacW}bratk06028
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General Information
Symbol
Dmel\P{lacW}bratk06028
Species
D. melanogaster
Name
FlyBase ID
FBti0007106
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k06028

Affected gene(s)
brat, Ecol\lacZ
Viability / fertility
Associated allele(s)
bratk06028, Ecol\lacZbrat-k06028
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2L ( 37C5 )
Sequence Location
2L:19,166,314..19,166,314 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:19,166,314..19,166,314 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
37C5 (inferred by FlyBase from sequence location)
Cytological location (reported)
37C6-37C7 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ025779
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 17
(Arama et al., 2000)
dorsal vessel wall cell
(Arama et al., 2000)
embryonic stage 12 -- 17
presumptive embryonic/larval peripheral nervous system

Comment: late embryonic stage 12

(Arama et al., 2000)
embryonic ventral nerve cord

Comment: late embryonic stage 12

(Arama et al., 2000)
embryonic brain

Comment: late embryonic stage 12

(Arama et al., 2000)
embryonic stage 13 -- 16
embryonic ganglion mother cell
(Arama et al., 2000)
embryonic stage 16
larval antenno-maxillary sensory complex
(Arama et al., 2000)
peripheral nervous system precursor cluster
(Arama et al., 2000)
third instar larval stage
gastric caecum
(Arama et al., 2000)
salivary gland imaginal ring
(Arama et al., 2000)
embryonic/larval proventriculus | posterior
(Arama et al., 2000)
larval ventral nerve cord | restricted
(Arama et al., 2000)
larval brain | restricted
(Arama et al., 2000)
ring gland
(Arama et al., 2000)
Additional Information
Statement
Reference

Reporter expression is detected in the developing brain, the ventral CNS, and the PNS starting at stage 11. Expression is detected in the ganglion mother cells starting at stage 13 and in 4 clusters of peripheral neurons at stage 16. Reporter expression is seen in cardioblasts from stage 11 to 17, which was not detected by brat in situ hybridization. Expression in the larval brain is limited to the inner region and may correspond to glia.

(Arama et al., 2000)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k06028

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:19162243-19162244 confirmed by FlyBase alignment of dbGSS accession AQ025779 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}bratk06028
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
l(2)k06028
(Lee et al., 2006)
Name Synonyms
Secondary FlyBase IDs
    References (20)