FlyBase Insertion Report: Dmel\P{GawB}NP6510

General Information

Symbol

Dmel\P{GawB}NP6510

Species

D. melanogaster

Name

FlyBase ID

FBti0037001

Feature type

transposable_element_insertion_site

Also Known As

NP6510, NP6510-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP6510

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 73E5 )

Sequence Location

3L:17,049,509..17,049,509 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

(Young and Armstrong, 2010)

Sequence Location

3L:17,049,509..17,049,509 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

73E5 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG219003

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

(Young and Armstrong, 2010)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Aso et al., 2012, Kahsai et al., 2010, Pauls et al., 2010, Xiong et al., 2010, Young and Armstrong, 2010, Donlea et al., 2009, Li et al., 2009, Tanaka et al., 2008, Liu et al., 2006)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP6510

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fan-shaped body layer 1 tangential neuron

(Ruppert et al., 2017, Cheng et al., 2013, Li et al., 2009)

mushroom body pedunculus-medial lobe and vertical lobe arborizing neuron 1

(Flybrain Neuron Database et al., 2018.6.28, Sun et al., 2018, Tanaka et al., 2008)

mushroom body alpha lobe posterior layer

(Tanaka et al., 2008)

mushroom body alpha lobe surface layer

(Tanaka et al., 2008)

mushroom body alpha lobe core layer

(Tanaka et al., 2008)

mushroom body alpha lobe slice 1

(Tanaka et al., 2008)

mushroom body beta lobe posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe surface layer

(Tanaka et al., 2008)

mushroom body beta lobe core layer

(Tanaka et al., 2008)

mushroom body beta lobe slice 1

(Tanaka et al., 2008)

pedunculus of adult mushroom body

(Tanaka et al., 2008)

fan-shaped body | ventral

(Young and Armstrong, 2010)

adult lateral fan-shaped body tangential-protocerebrum neuron ExFl1

(Sun et al., 2020, Flybrain Neuron Database et al., 2018.6.28, Weir et al., 2014, Young and Armstrong, 2010)

adult dopaminergic PAM neuron | subset

(Agrawal and Hasan, 2015, Aso et al., 2012)

adult mushroom body beta-lobe | restricted

(Riemensperger et al., 2013)

adult mushroom body beta'-lobe | restricted

(Riemensperger et al., 2013)

fan-shaped body | restricted

(Riemensperger et al., 2013)

adult antennal lobe | restricted

(Ruppert et al., 2017)

adult mushroom body | restricted

(Ruppert et al., 2017)

ellipsoid body | restricted

(Ruppert et al., 2017)

adult salivary gland

(Ruppert et al., 2017)

alpha'/beta' anterior-posterior Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

adult dopaminergic PAM neuron

(Riemensperger et al., 2013)

Additional Information

Statement

Reference

Drives expression in non-dopaminergic neurons in the fan-shaped body, including the adult lateral fan-shaped body tangential-protocerebrum neuron ExFl1; and in a subset of 15 dopaminergic PAM neurons that innervate beta- and beta'-lobes of the adult mushroom body.

(FlyBase Curators, 2017)

ScerGAL4^NP6510 drives expression in a subset of 15 dopaminergic PAM neurons that innervate beta- and beta'-lobes of the adult mushroom body. Projections are observed both to the ipsilateral and the contralateral side. Neurons innervating the beta'-lobe show extensive dendritic arborization. Two to three labelled neurons innervating the fan-shaped body are non-dopaminergic. GRASP experiments show that ScerGAL4^NP6510 expression is presynaptic in Kenyon cells of the mushroom body medial lobes.

(Riemensperger et al., 2013)

The number of neurons detected by ScerGAL4^NP6510 varied between hemispheres of the same brain.

(Cheng et al., 2013)

ScerGAL4^NP6510 drives expression in a subset of dopaminergic PAM neurons that innervate the mushroom body beta' lobe segment 2, beta lobe segment 1 and alpha lobe segment 1.

(Aso et al., 2012)

Approximately 7 fan-shaped F1 somata are labelled with ScerGAL4^NP6510. Neurites from these cells converge into a bundle that runs dorsally and the posteriorly into the fan-shaped body.

(Li et al., 2009)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Riemensperger et al., 2013, Aso et al., 2012, Young and Armstrong, 2010, Li et al., 2009, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Avic\GFP^{spGFP1-10.UAS.Tag:M(hCD4)}

(Riemensperger et al., 2013)

Avic\GFP^2x.EGFP.UAS

(Weir et al., 2014)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

113956

y^* w^*; P{GawB}NP6510 / TM6, P{UAS-lacZ.UW23-1}UW23-1

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG219003

Comments

Location 3L:17014767-17014768 refined by FlyBase alignment of dbGSS accession AG219003 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Gal4-NP6510

(Li et al., 2009)

NP6510

(Sun et al., 2020, Weir et al., 2014, Cheng et al., 2013, Kottler et al., 2013, Aso et al., 2012, Aso et al., 2010, Kahsai et al., 2010, Pauls et al., 2010, Young and Armstrong, 2010, Donlea et al., 2009, Hong et al., 2008, Tanaka et al., 2008, Liu et al., 2006)

NP6510-GAL4

(Majcin Dorcikova et al., 2023, Ruppert et al., 2017, Xiong et al., 2010)

NP6510-Gal4

(Kajtor et al., 2025, Petitgas et al., 2024, Rahmani et al., 2022, Pütz et al., 2021, Sun et al., 2018, Riemensperger et al., 2013)

NP6510GAL4

(Agrawal and Hasan, 2015)

Np6510

(Hong et al., 2008)

P{GawB}NP6510

(Weir et al., 2014, FlyBase, 1992-)

P{GawB}NP6510-3-1

(FlyBase, 2005, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (27)

Report Sections

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