FlyBase Insertion Report: Dmel\P{GawB}tutl[GAL4]

General Information

Symbol

Dmel\P{GawB}tutl^GAL4

Species

D. melanogaster

Name

FlyBase ID

FBti0129743

Feature type

transposable_element_insertion_site

Also Known As

tutl-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, tutl

Viability / fertility

Associated allele(s)

Scer\GAL4^tutl-GAL4, tutl^GAL4

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 24E1 )

Sequence Location

2L:4,303,444..4,303,444

Target / Docking site

P{PZ}tutl⁰¹⁰⁸⁵

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:4,303,444..4,303,444

(Ferguson et al., 2009)

Orientation

Cytological location (computed by FlyBase)

24E1 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Same insertion site as the progenitor P{PZ}tutl⁰¹⁰⁸⁵ insertion.

(Ferguson et al., 2009)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Ferguson et al., 2009)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Tsubouchi et al., 2012, Ferguson et al., 2009)

tutl

(Ferguson et al., 2009)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^tutl-GAL4

Phenotypic Class Phenotype Manifest in Detailed Description

tutl^GAL4

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset of larval central nervous system

(Zhou et al., 2012)

larval multidendritic class III neuron

Comment: not expressed in ddaF neurons

(Tsubouchi et al., 2012, Zhou et al., 2012)

larval dorsal multidendritic neuron ddaF

Comment: high levels

(Sulkowski et al., 2011)

larval dorsal multidendritic neuron ddaC

Comment: high levels

(Sulkowski et al., 2011)

larval dorsal multidendritic neuron ddaA

Comment: high levels

(Sulkowski et al., 2011)

larval dorsal multidendritic neuron ddaB

Comment: intermediate levesl

(Sulkowski et al., 2011)

larval dorsal multidendritic neuron ddaD

Comment: low levels

(Sulkowski et al., 2011)

larval dorsal multidendritic neuron ddaE

Comment: low levels

(Sulkowski et al., 2011)

adult stage

photoreceptor cell R7

(Ferguson et al., 2009)

medulla | restricted

(Ferguson et al., 2009)

adult optic lobe | restricted

(Ferguson et al., 2009)

Additional Information

Statement

Reference

The analysis was focused on the dorsal cluster of da neurons in third instar larvae. The highest level of tutl-GAL4 driven GFP expression is observed in the dorsal-most class III da neuron (ddaF) followed by the class IV da neuron (ddaC) and the ventral-mostclass III da neuron (ddaA). Intermediate levels of expression are observed in the class II da neuron (ddaB) and very low levels are observed in the two class I da neurons (ddaD and ddaE). Expression is also observed in other sensory neurons of the dorsal cluster.

(Sulkowski et al., 2011)

tutl^GAL4 is expressed in some non-R7 axons in the medulla neuropil and inner optic lobe.

(Ferguson et al., 2009)

Marker for

Reflects expression of

tutl

(Tsubouchi et al., 2012, Zhou et al., 2012, Sulkowski et al., 2011)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tsubouchi et al., 2012, Zhou et al., 2012)

Avic\GFP^{tdGFP.UAS.Tag:M(hCD4)}

(Zhou et al., 2012)

tutl^RNAi.400.UAS

(Ferguson et al., 2009)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Recombinant descendant of (donor)

P{GawB}elav^C155

(Ferguson et al., 2009)

Replacement descendant of (target)

P{PZ}tutl⁰¹⁰⁸⁵

(Ferguson et al., 2009)

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

The P{Z} element in P{PZ}tutl⁰¹⁰⁸⁵ has been replaced with a P{GawB} element (using P{GawB}elav^C155 as the donor).

(Ferguson et al., 2009)

The orientation of the insertion was not reported.

(Ferguson et al., 2009)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{GAL4}tutl^GAL4

(FlyBase, 1992-)

P{GawB}tutl^GAL4

tutl-GAL4

(Tsubouchi et al., 2017, Tsubouchi et al., 2012, Zhou et al., 2012, Sulkowski et al., 2011)

tutl^GAL4

(Ferguson et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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