FlyBase Insertion Report: Dmel\P{GawB}esg[NP7397]

General Information

Symbol

Dmel\P{GawB}esg^NP7397

Species

D. melanogaster

Name

FlyBase ID

FBti0229280

Feature type

transposable_element_insertion_site

Also Known As

esg-Gal4, esg-GAL4^NP7397, esgGAL4, esg^NP7397-Gal4, esg^ts-Gal4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

esg, Scer\GAL4

Viability / fertility

Associated allele(s)

esg^NP7397, Scer\GAL4^NP7397

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 35D1 )

Sequence Location

2L:15,333,740..15,333,740 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:15,333,740..15,333,740 [+]

(FlyBase, 2005)

2L:15,333,740..15,333,740 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

35D1 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG219145

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

(FlyBase, 1992-)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

esg

(Jiang and Edgar, 2009)

Scer\GAL4

(Kohlmaier et al., 2015, Perdigoto et al., 2011, Jiang and Edgar, 2009)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

esg^NP7397

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^NP7397

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- pupal stage P5

midgut imaginal island

(Jiang and Edgar, 2009)

third instar larval stage

histoblast

(Malzer et al., 2018)

imaginal disc

(Malzer et al., 2018)

larval central nervous system

(Malzer et al., 2018)

embryonic/larval gut

(Malzer et al., 2018)

pupal stage P6

intestinal stem cell

(Jiang and Edgar, 2009)

adult stage

enteroblast

(Jiang et al., 2011)

intestinal stem cell

(Jiang et al., 2011)

intestinal stem cell of posterior adult midgut epithelium

(Hudry et al., 2016)

enteroblast of posterior adult midgut epithelium

(Hudry et al., 2016)

adult stage | male

male accessory gland secondary cell

(Corrigan et al., 2014, Ito et al., 2014)

Additional Information

Statement

Reference

ScerGAL4^NP7397 drives larval expression in the gut, CNS, imaginal discs, and histoblasts.

(Malzer et al., 2018)

Drives expression in enteroblasts and intestinal stem cells; also drives expression in imaginal discs.

(FlyBase Curators, 2017)

ScerGAL4^esg-NP7397 drives expression in the adult midgut precursor cells and intestinal stem cells.

(Jiang and Edgar, 2009)

Marker for

enteroblast of posterior adult midgut epithelium

(Hudry et al., 2016)

intestinal stem cell of posterior adult midgut epithelium

(Hudry et al., 2016)

Reflects expression of

esg

(Malzer et al., 2018, Hudry et al., 2016, Corrigan et al., 2014, Ito et al., 2014, Jiang et al., 2011, Jiang and Edgar, 2009)

Reporter construct used in assay

Avic\GFP^UAS.cUa

(Jiang and Edgar, 2009)

Avic\GFP^Stat92E.10X

(Jiang et al., 2011)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

114268

w^*; P{GawB}esg^NP7397 / CyO, P{UAS-lacZ.UW14}UW14

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG219145

Comments

Location 2L:15329668-15329669 refined by FlyBase alignment of dbGSS accession AG219145 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

NP7397

(Jiang and Edgar, 2009)

P{GawB}NP7397

(FlyBase, 1992-)

P{GawB}NP7397-5-1

(FlyBase, 2005, FlyBase, 1992-)

P{GawB}esg^NP7397

(FlyBase, 1992-)

escargot^NP7397-Gal4

(Malzer et al., 2018)

esg-GAL4

(Marie et al., 2023)

esg-GAL4^NP7397

(Corrigan et al., 2014)

esg-Gal4

(Blackie et al., 2024, Bohere et al., 2022, Na et al., 2022, Mattila et al., 2018, Perea et al., 2017, Hudry et al., 2016, Patel et al., 2015, Zielke et al., 2014, Jiang et al., 2011)

esg-Gal4^NP7397

(Delanoue et al., 2023, Banreti et al., 2022, Hudry et al., 2019, Perea et al., 2017)

esg-gal4^np7397

(Hudry et al., 2016)

esg^NP7397-Gal4

(Blackie et al., 2024, Zielke et al., 2014)

esg^ts

(Tian et al., 2017)

esg^ts-Gal4

(Wang et al., 2024, Deliu et al., 2022)

esg^tsGal4

(Liu and Jin, 2017)

esgGAL4

(Malzer et al., 2018, Perdigoto et al., 2011)

esgGal4

(Bohere et al., 2022, Lee et al., 2016, Kohlmaier et al., 2015)

esgGal4^NP7397

(Jiang and Edgar, 2009)

Name Synonyms

Secondary FlyBase IDs

FBti0037446
FBti0139914

References (28)

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