FB2026_02 , released June 18, 2026
Recombinant Construct: P{GAL4-nanos.NGT}
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General Information
Symbol
P{GAL4-nanos.NGT}
FlyBase ID
FBtp0001612
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4nanos.PG
Also Known As
nos-Gal4, nanos-Gal4, NGT40, MTD-GAL4, P{GAL4-nos.NGT}, NGT, NGT-Gal4, nosGal4, NGT40-Gal4, GAL4-nos.NGT
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
5 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Scer\GAL4nanos.PG
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
nanos_nos-NGT
Encoded product / tool
GAL4
Description

The nos promoter regulates expression of a GAL4 driver. The αTub84B 3' UTR is fused downstream of the GAL4 coding region.

(Tracey et al., 2000)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Barrett et al., 1997)
embryonic cycle 12 -- embryonic cycle 13
organism | restricted
(Tracey et al., 2000)
embryonic stage 5 -- 11
organism | uniform
(Tracey et al., 2000)
embryonic stage 13 -- 14
cell | subset

Comment: a few cells dorsal to the yolk sac

(Tracey et al., 2000)
adult stage
female germline cell
(McKinney et al., 2020)
oogenesis & adult stage | female
female germline cell
(Bloomington Drosophila Stock Center, 2017)
Additional Information
Statement
Reference

ScerGAL4nos.PG drives expression in the female germ line; expressed gene product is loaded into oocytes

(Bloomington Drosophila Stock Center, 2017)

Drives expression in the germ line in female adults; expressed gene product is loaded into oocytes. Commonly used together with Scer\GAL4VP16.nanos.UTR and Scer\GAL4VP16.otu to form a combination referred to as the "maternal triple driver" (MTD-GAL4).

(FlyBase Curators, 2017)
 
Marker for
female germline cell
(McKinney et al., 2020)
Reflects expression of
nanos
(McKinney et al., 2020, Bloomington Drosophila Stock Center, 2017, Tracey et al., 2000)
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Obtained from P. Gergen.Designed to provide expression early in embryogenesis.

(Barrett et al., 1997)

construct_comment: Progenitors are those of the plasmid, pP{GAL4-nos.NGT}.

(Bloomington Drosophila Stock Center, 1998.9.14)

report comment: nos promoter driving GAL4, 3\' UTR from a maternally expressed α-tubulin.

(Bloomington Drosophila Stock Center, 1998.9.14)
Stocks (14)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (79)
Reported As
Symbol Synonym
GAL4-NosNGTA
(Park et al., 2019)
GAL4-nos.NGT
(Ng et al., 2020, Lieber et al., 2019, Crocker and Stern, 2013, Pérez-Montero et al., 2013)
GAL4-nos.NGT40
(Rashpa et al., 2017)
Gal4(nos-NGT40)
(Rogers et al., 2008)
Gal4-nos.NGT
(Ma et al., 2020)
Gal4-nos.NGT40
(Bishop et al., 2023)
MTD
(Jiang et al., 2015, Levine et al., 2015, Liu et al., 2015, Xu et al., 2014)
MTD-GAL4
(Hocaoglu et al., 2021, Blundon et al., 2016, Catrina et al., 2016, Hayashi et al., 2016, Hayashi et al., 2014, Urban et al., 2014, Azzam and Liu, 2013, Dix et al., 2013, Olivieri et al., 2012, Handler et al., 2011)
MTD-Gal4
(Chan and Pek, 2023, Shao et al., 2022, ElMaghraby et al., 2019, Kong et al., 2019, Murano et al., 2019, Ng et al., 2018, Goyal et al., 2017, Kan et al., 2017, Song et al., 2017, Börner and Becker, 2016, Börner et al., 2016, Huang et al., 2016, Kuhn et al., 2015, Little et al., 2015, Pek et al., 2015, Senti et al., 2015, Sienski et al., 2015, Winkler et al., 2015, Cai et al., 2014, Wei et al., 2014)
MTDGal4
(Jankovics et al., 2018)
Maternal Triple Driver
(Rodrigues et al., 2016)
NGT
(Chen et al., 2025, Kenny et al., 2021, Prazak et al., 2021, Mondragon et al., 2019, Xu et al., 2014, Etchegaray et al., 2012, Handler et al., 2011, Wang and Elgin, 2011, Joyce and McKim, 2010, Bass et al., 2009, Hou et al., 2008, Wang et al., 2007, Rosenberg and Parkhurst, 2002)
NGT-40
(Noguchi and Miller, 2003)
NGT-GAL4
(Baumgartner et al., 2022, Dönertas et al., 2013, LaLonde et al., 2006)
NGT-Gal4
(Azlan and Fukunaga, 2025, Azlan et al., 2025, Andreev et al., 2022, ElMaghraby et al., 2022, Chen et al., 2019, Genenncher et al., 2018, Bence et al., 2017, Pandey et al., 2017, Henriksen et al., 2002)
NGT4
(Zimmermann et al., 2006)
NGT4-gal4
(Zimmermann et al., 2006)
NGT40
(Li et al., 2024, Xu et al., 2024, Monteiro et al., 2023, Suyama et al., 2023, Lim et al., 2022, Bell et al., 2020, Kawaguchi et al., 2020, Lu et al., 2013, Pek et al., 2012, Degennaro et al., 2011, Shimada et al., 2011, Patil and Kai, 2010, Hou et al., 2009, Pek et al., 2009, Chen et al., 2008, Baum et al., 2007, Clark et al., 2007, Lee and Cooley, 2007, Murray and Saint, 2007, Yamashita et al., 2007, Yamashita et al., 2007, LaLonde et al., 2006, Mazzalupo and Cooley, 2006, Zimmermann et al., 2006, Hatfield et al., 2005, Cox and Spradling, 2003, Vander Zwan et al., 2003, Jackson and Berg, 2002, Wheeler et al., 2002, Yussa et al., 2001, Grieder et al., 2000, Li and Gergen, 1999)
NGT40 GAL4
(Delanoue and Davis, 2005)
NGT40-GAL4
(Banerjee et al., 2021, Bowler et al., 2006, Yoshida et al., 2001)
NGT40-Gal4
(Matsui et al., 2026, Lin et al., 2023, Jussen et al., 2016, Urbach et al., 2016, Hernandez et al., 2005)
NGT40-Gal4-VP16
(Osman and Pek, 2021)
NGT40-gal4
(Zimmermann et al., 2006)
NGT40/GAL4
(Hou et al., 2009)
NGT40:Gal4
(Rosales-Nieves et al., 2006)
NGT::Gal4
(Hain et al., 2014)
NGTA
(Vander Zwan et al., 2003)
Nanos-GAL4
(Pachinger et al., 2025, Dobbelaere et al., 2023)
Nanos-Gal4
(Houston et al., 2020)
Nos-GAL4
(Lie-Jensen et al., 2019, Gerstberger et al., 2017)
Nos-GAL4.NGT
(Lie-Jensen et al., 2019)
Nos-Gal4
(Shinoda et al., 2023, Kairamkonda and Nongthomba, 2018, Iovino et al., 2013, Wang et al., 2011, Singh et al., 2006)
Nos-gal4
(Chen et al., 2025)
Nos:Gal4
(Klein et al., 2016)
P(GAL4-nos.NGT)
(Walrad et al., 2011)
P(GAL4-nos.NGTA)
(Walrad et al., 2011)
P(GAL4-nosNGT)
(Dix et al., 2013)
P(Gal4-nos.NGT)
(Joyce and McKim, 2010)
Pw(+mC)=GAL4-nos.NGT40
(Coffee et al., 2010)
P{GAL4-nanos.NGT}
(Reim et al., 2003, FlyBase, 1996-)
P{GAL4-nos.NGT}
(Fellmeth et al., 2023, Kordyukova et al., 2020, Akishina et al., 2017, Bence et al., 2017, Nakamura et al., 2017, Olovnikov et al., 2016, Herzig et al., 2014, Hill et al., 2014, Hodar et al., 2014, Dubin-Bar et al., 2011, Shpiz et al., 2011, Prazak et al., 2010, Zúñiga et al., 2009, Horne-Badovinac and Bilder, 2008, Vanderzwan-Butler et al., 2007, LaLonde et al., 2006, FlyBase, 1996-)
P{GAL4-nos.NGT}40
(Morgunova et al., 2021)
P{Gal4-nos.NGT}
(Yamada et al., 2019, Petrella et al., 2007, Hozumi et al., 2006)
P{nosGAL4= w+mC}
(Brower-Toland et al., 2009)
gal4-nos
(Zhang et al., 2023)
maternal GAL4
(Winkler et al., 2015)
nanos GAL4
(Handler et al., 2013, Coffee et al., 2010)
nanos Gal-4
(Deshpande et al., 2001)
nanos-GAL4
(Henarejos-Castillo et al., 2024, Mondragon et al., 2019, Zhao et al., 2019, Matias et al., 2015, Yu et al., 2015, Urban et al., 2014, Etchegaray et al., 2012, Biersmith et al., 2011, Bakhrat et al., 2010, Bass et al., 2009, Zúñiga et al., 2009, Mische et al., 2008, Gonsalvez et al., 2006, Sanny et al., 2006, Ward et al., 2006, Murthy et al., 2005, Noguchi and Miller, 2003, Timinszky et al., 2002)
nanos-Gal4
(Goldstein et al., 2025, Shima et al., 2025, François et al., 2023, Xu et al., 2022, Bestetti et al., 2021, Villa-Fombuena et al., 2021, Dattoli et al., 2020, Kairamkonda and Nongthomba, 2018, Neitzel et al., 2018, Dominguez et al., 2016, Natalizio and Matera, 2013, Patil and Kai, 2010, Chen et al., 2008, Kamimura et al., 2006, Morris et al., 2006, Siller et al., 2005, Cook et al., 2004, Wang et al., 2004)
nanos-Gal4-NGT
(Charron et al., 2008)
nanos-Gal4-tubulin
(Etchegaray et al., 2012, Charron et al., 2008)
nanos.NGT-Gal4
(Brand et al., 2024)
nanos>Gal4
(Kristó et al., 2024, Trevino et al., 2021)
nanosGAL4
(Shcherbata et al., 2007)
nos Gal4
(Rashpa et al., 2017)
nos*
(Monk et al., 2010)
nos-GAL4
(Eldridge-Thomas et al., 2025, Liu et al., 2025, Stowers, 2025, Collinet et al., 2024, Scanlan and Robin, 2024, Klucnika et al., 2023, Monteiro et al., 2023, Certel et al., 2022, Certel et al., 2022, Fefelova et al., 2022, Almeida-Oliveira et al., 2021, Stark et al., 2021, McKinney et al., 2020, Stolyarenko, 2020, Tison et al., 2020, Yoshinari et al., 2020, Fabry et al., 2019, Lieber et al., 2019, Sênos Demarco et al., 2019, Williams et al., 2019, Yang et al., 2019, Ameku et al., 2018, Mageeney et al., 2018, Nakamura et al., 2017, Czech et al., 2013, Chen et al., 2010, Coffee et al., 2010, Papaconstantinou et al., 2010, Moon et al., 2008, Deshpande et al., 2007, Papaconstantinou et al., 2005, Takemaru et al., 2003)
nos-GAL4-tubulin
(Shropshire and Bordenstein, 2019)
nos-Gal4
(Hirai et al., 2025, Lee et al., 2025, Rosales-Nieves et al., 2025, Shima et al., 2025, Kong et al., 2024, Taslim et al., 2023, Wang et al., 2023, Wenzel and Aquadro, 2023, Eslamieh et al., 2022, Kogan et al., 2022, Bosch et al., 2021, Eastwood et al., 2021, Dai et al., 2020, Nakamura et al., 2020, Hudry et al., 2019, Luo et al., 2019, Wang et al., 2019, Neitzel et al., 2018, Dominguez et al., 2016, Kotov et al., 2016, Hain et al., 2014, Herzig et al., 2014, Hodar et al., 2014, Wei et al., 2014, Czech et al., 2013, Dubin-Bar et al., 2011, Di Cara et al., 2010, Wang and Lin, 2007, Morris et al., 2006, Murthy and Schwarz, 2004, Niessing et al., 2000)
nos-Gal4.NGT
(Xin et al., 2013)
nos-NGT-GAL4
(Belacortu et al., 2011)
nos-gal-4
(Liu et al., 2015)
nos-gal4
(Gui et al., 2023, Chen et al., 2018)
nos-gal4 tub-3'UTR
(Pozmanter et al., 2025)
nos. NGT GAL4
(Saha et al., 2023)
nos.NGT-Gal4
(Taniguchi and Igaki, 2023)
nos::GAL4
(Kneuss et al., 2019, Munafò et al., 2019, Crocker and Stern, 2013, Krahn et al., 2010)
nos:GAL4
(Sarikaya and Extavour, 2015)
nos:Gal4
(Monk et al., 2010)
nosG4
(Liu et al., 2024)
nosGAL4
(Breznak et al., 2023, Sarkar et al., 2023, Martin et al., 2022, McCarthy et al., 2022, Blatt et al., 2021, Flora et al., 2018, Zhao et al., 2013)
nosGAL4.NGT
(Palumbo et al., 2022, Flora et al., 2018)
nosGal4
(Chen et al., 2023, Kordyukova et al., 2018, Compagnon et al., 2009, Yu et al., 2009)
nosNGT-Gal4
(Genenncher et al., 2018)
nosP-GAL4
(Sanchez et al., 2016)
nosP-Gal4-NGT40
(Obata et al., 2018)
pGAL4-nos.NGT40
(Barry et al., 2011)
pnos-Gal4-tubulin 3'UTR
(Degennaro et al., 2011)
Secondary FlyBase IDs
    References (318)