FlyBase Recombinant Construct Report: P{GAL4-lacZ-Ubx(FRT.f[+]).dC}

An FRT cassette that contains a f^+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)

Component allele Ecol\lacZ^FRT.Ubx

Transgenic product class

Component

Use(s)

Regulatory region(s)

Ubx

Encoded product / tool

lacZ

reporter enzyme

Also carries

FRT

recombinase target site

Description

An FRT cassette that contains a f^+t13 marker separates the Ubx promoter from a coding cassette composed of GAL4 and lacZ (the two coding regions are separated by an internal ribosome entry site).

(de Celis and Bray, 1997)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^FRT.Ubx

Phenotypic Class Phenotype Manifest In Detailed Description

Scer\GAL4^FRT.Ubx

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Designed for clonal analysis in wing disc and adult wing; FRT-containing variant blocks ScerGAL4 transcription. Expression is of ScerGAL4^Ubx.PdC, carried in descendent transposon P{GAL4-lacZ-Ubx(-FRT).dC}; see ScerGAL4^Ubx.PdC elsewhere in this table.

(FlyBase Curators, 2017)

Used as marker for clonal analysis in wing; FRT-containing variant blocks EcollacZ expression; see expression data for P{GAL4-lacZ-Ubx(-FRT).dC} at EcollacZ^Ubx.PdCRA.

(de Celis and Bray, 1997)

Used for clonal analysis in wing; FRT-containing variant blocks ScerGALZ expression; see expression data for P{GAL4-lacZ-Ubx(-FRT).dC} at ScerGAL4^Ubx.PdCRA.

(de Celis and Bray, 1997)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

P{GAL4-lacZ-Ubx(-FRT).dC}

(de Celis and Bray, 1997)

Comments

construct_comment: Designed for clonal analysis in wing disc and adult wing: Ser\GAL4 expressed in cells that have lost the FRT.f⁺ element; used for clonal analysis the gene of interest, when appropriate UAS construct present; clones are simultaneously marked by expression of Ecol\lacZ in the imaginal disc and by f^- phenotype in the adult wing.

(de Celis and Bray, 1997)

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

36526

P{GAL4-lacZ-Ubx(FRT.f⁺).dC}1, y¹ w¹ f^36a; P{UAS-FLP.D}JD1

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

10.1E

(Rauskolb and Irvine, 1999)

(Li and Baker, 2004)

P[abx/Ubx<FRT f⁺FRT>Gal4-lacZ]

(Baonza et al., 2000)

P{GAL4-lacZ-Ubx(FRT.f⁺).dC}

(FlyBase, 1996-)

P{GAL4-lacZ.FRT.dC}

(FlyBase, 1996-)

P{abx/Ubx<FRTf⁺FRT>Gal4-lacZ}

(de Celis and Bray, 2000)

Ubx-FRT<f⁺>Gal4

(Tree et al., 2002)

Ubx/abxFRT f⁺ FRT Gal4 lacZ

(Azpiazu and Morata, 2002)

Ubx<f⁺>GAL4-lacZ

(Whitworth and Russell, 2003)

Ubx> stop f⁺ >GAL4

(Apidianakis et al., 1999)

Ubx>f⁺>Gal4-lacZ

(Phillips et al., 1999)

Ubx>stop f+ >GAL4 lacZ

(Sakata, 2004)

ab>f⁺>Gal4-lacZ

(Cook et al., 2004)

abx/Ubx FRT f⁺ FRT G4 UAS lacZ

(Baena-Lopez and Garcia-Bellido, 2003)

abx/Ubx>stop,f⁺>GAL4-lacZ

(FlyBase, 1996-)

abx/pbx>f⁺>Gal4-lacZ

(Lawrence et al., 1999)

abx/ubx>f+>Gal4-lacZ

(Lawrence et al., 2004, Casal et al., 2002)

abx/ubx>f⁺>Gal4-lacZ

(Dominguez, 1999)

abx/ubx>f⁺Gal4-lacZ

(Lawrence et al., 2002)

abx>f⁺-stop>Gal4-lacZ

(Calleja et al., 2000)

abx>f⁺>Gal4-lacZ

(Moreno et al., 2002, Azpiazu and Morata, 2000)

abxubs>FRT>Gal4

(Hsiung, 2005.9.23)

abxubx>FRT>GAL4

(Roy et al., 2011)

abxubx>FRT>Gal4

(Hsiung et al., 2005)

pwA>f>GIL

(FlyBase, 1996-)

ubx>f+>Gal4

(Torroja et al., 2004)

ubx>f⁺>Gal4, UAS-βgal

(Callejo et al., 2006)

Secondary FlyBase IDs

References (33)

Report Sections

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