FB2026_02 , released June 18, 2026
Recombinant Construct: P{ChAT-GAL4.DBD}
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General Information
Symbol
P{ChAT-GAL4.DBD}
FlyBase ID
FBtp0023500
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4DBD.ChAT
Also Known As
cha-Gal4DBD
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Scer\GAL4DBD.ChAT
Transgenic product class
Component
Use(s)
Regulatory region(s)
ChAT
Encoded product / tool
GAL4(DBD)::Zip-
Description

A ChAT promoter regulates expression of a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Luan et al., 2006)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
lamina monopolar neuron L2

Comment: when combined with P{ort-GAL4.AD.C1-3}

(Gao et al., 2008)
transmedullary neuron Tm2

Comment: when combined with P{ort-GAL4.AD.C1-3}

(Gao et al., 2008)
transmedullary neuron Tm20

Comment: when combined with P{ort-GAL4.AD.C1-3}

(Gao et al., 2008)
transmedullary neuron Tm9

Comment: when combined with P{ort-GAL4.AD.C1-3}

(Gao et al., 2008)
T4 neuron

Comment: when combined with P{R42F06-p65.AD}

(Mauss et al., 2014)
T5 neuron

Comment: when combined with P{R42F06-p65.AD}

(Mauss et al., 2014)
adult stage | male
adult male copulation reporting neuron

Comment: when combined with P{R42G02-p65.AD}

(Zhang et al., 2019)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
ChAT
(Mauss et al., 2014, Gao et al., 2008)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
23869
w*; P{UAS-2xEGFP}AH2; P{ChAT-GAL4.DBD}J8A1
56516
w*; P{ort-GAL4.AD.C1-3}20; P{ChAT-GAL4.DBD}J8A1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
Reported As
Symbol Synonym
CHAT-Gal4DBD
(Zhang et al., 2019)
ChAT-DBD
(Banerjee et al., 2025)
Cha(7.4kb)Gal4.DBD
(Couton et al., 2015)
Cha-DBD
(Mauss et al., 2014)
Cha-GAL4DBD
(Dolan et al., 2017)
Cha-Gal4-DBD
(Kohl et al., 2013)
ChaDBD
(Dolan et al., 2017)
P{ChAT-GAL4.DBD}
(FlyBase, 1996-)
P{Cha-GAL4.DBD}
(FlyBase, 1992-)
cha-Gal4DBD
(Gao et al., 2008, Gao et al., 2008)
pCAST-ChaGal4DBD
(Luan et al., 2006)
Secondary FlyBase IDs
    References (13)