Reference Report

Reference
Citation	Ronshaugen, M., Biemar, F., Piel, J., Levine, M., Lai, E.C. (2005). The Drosophila microRNA iab-4 causes a dominant homeotic transformation of halteres to wings. Genes Dev. 19(24): 2947--2952. (Export to RIS)
FlyBase ID	FBrf0190560
Publication Type	Research paper
PubMed ID	16357215
PubMed Abstract	The Drosophila Bithorax Complex encodes three well-characterized homeodomain proteins that direct segment identity, as well as several noncoding RNAs of unknown function. Here, we analyze the iab-4 locus, which produces the microRNAs iab-4-5p and iab-4-3p. iab-4 is analogous to miR-196 in vertebrate Hox clusters. Previous studies demonstrate that miR-196 interacts with the Hoxb8 3' untranslated region. Evidence is presented that miR-iab-4-5p directly inhibits Ubx activity in vivo. Ectopic expression of mir-iab-4-5p attenuates endogenous Ubx protein accumulation and induces a classical homeotic mutant phenotype: the transformation of halteres into wings. These findings provide the first evidence for a noncoding homeotic gene and raise the possibility that other such genes occur within the Bithorax complex. We also discuss the regulation of mir-iab-4 expression during development.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (10)
	Avic\GFP^{αTub84B.Ubx.3'UTR} mir-iab-4^{Scer\UAS.cRa.T:Disc\RFP-DsRed2} Pc¹ Scer\GAL4^Bx-MS1096 Scer\GAL4^ptc-559.1 Scer\GAL4^sd-SG29.1 T:Disc\RFP^DsRed2 Ubx^61d Ubx^bx-34e Ubx^pbx-1
Constructs (2)
	P{tub-GFP.Ubx.3'UTR} P{UAS-DsRed2-mir-iab-4.R}
Genes (18)
	abd-A Abd-B Avic\GFP Dana\Ubx Dmoj\Ubx Dpse\mir-iab-4 Dpse\Ubx Dvir\Ubx Dyak\Ubx Fab-7 Fab-8 iab-4 iab-8 MCP-PRE mir-iab-4 Pc Scer\GAL4 Ubx
Insertions (3)
	P{GawB}Bx^MS1096 P{GawB}ptc^559.1 P{GawB}sd^SG29.1
Natural transposons (1)
	P-element