FB2025_01 , released February 20, 2025
Reference Report
Open Close
Reference
Citation
Kumari, A., Ghosh, A., Kolay, S., Raghu, P. (2022). Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein-coupled PLC signalling in vivo.  Life Sci Alliance 5(6): e202101293.
FlyBase ID
FBrf0252936
Publication Type
Research paper
Abstract
Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] hydrolysis by phospholipase C (PLC) is a conserved mechanism of signalling. Given the low abundance of PI(4,5)P2, its hydrolysis needs to be coupled to resynthesis to ensure continued PLC activity; however, the mechanism by which depletion is coupled to resynthesis remains unknown. PI(4,5)P2 synthesis is catalyzed by the phosphorylation of phosphatidylinositol 4 phosphate (PI4P) by phosphatidylinositol 4 phosphate 5 kinase (PIP5K). In Drosophila photoreceptors, photon absorption is transduced into PLC activity and during this process, PI(4,5)P2 is resynthesized by a PIP5K. However, the mechanism by which PIP5K activity is coupled to PI(4,5)P2 hydrolysis is unknown. In this study, we identify a unique isoform dPIP5KL, that is both necessary and sufficient to mediate PI(4,5)P2 synthesis during phototransduction. Depletion of PNUT, a non-redundant subunit of the septin family, enhances dPIP5KL activity in vitro and PI(4,5)P2 resynthesis in vivo; co-depletion of dPIP5KL reverses the enhanced rate of PI(4,5)P2 resynthesis in vivo. Thus, our work defines a septin-mediated mechanism through which PIP5K activity is coupled to PLC-mediated PI(4,5)P2 hydrolysis.
PubMed ID
PubMed Central ID
PMC8921834 (PMC) (EuropePMC)
Related Publication(s)
FlyBase analysis

Assignment of cell line based on information provided by the author in the Fast Track Your Paper tool.
FlyBase Curators, 2020-, Assignment of cell line based on information provided by the author in the Fast Track Your Paper tool. [FBrf0247694]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Life Sci Alliance
    Title
    Life science alliance
    ISBN/ISSN
    2575-1077
    Data From Reference