A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial set of 519 isogenic deletions provides 56% genome coverage.
The current Exelixis collection at the Bloomington Stock Center differs from the original described in FBrf0175003 : a significant number were shown not to carry a deletion and have been removed from the collection; a number of stocks have been lost; a number of additional deletions are included that were generated after publication.
Breakpoint based on release 5 sequence coordinate from FlyBase.
Breakpoint based on release 3 coordinate 2R:10739927 , per A. Parks (Exelixis Inc.) personal communication, from the hybrid element associated with the deficiency.
Heterozygosity for Df(2R)Exel6285 results in a significant reduction in evoked excitatory junctional potentials (EJPs) and quantal content (QC) recorded from muscles of homozygous Df(2R)KT40 mutant larvae.