Heterozygotes of ash11, ash16, and ash128 with brm2 have homeotic transformations of the third to the second leg. The expressivity of the transformed leg is variable, some have only a second leg apical bristle while others have a second leg preapical bristle as well. There is a lower penetrance of third leg transformations among flies heterozygous for ash11, ash16, and ash128 with brm5. Degree of penetrance corresponds to the severity of the ash1 allele (ash11 < ash16 < ash128). There is a lower penetrance of partial homeotic transformation of the haltere to the wing, or dorsal metathoracic cuticle to mesothoracic cuticle, and an even lower penetrance of first leg to second leg transformations.