FB2026_02 , released June 18, 2026
Allele: Dmel\sub1
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General Information
Symbol
Dmel\sub1
Species
D. melanogaster
Name
FlyBase ID
FBal0016600
Feature type
allele
Associated gene
Dmel\sub
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Schupbach and Wieschaus, 1989)
Progenitor genotype
Cytology
Description

Amino acid replacement: Q230term.

(Jang et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:17,747,230..17,747,230 [-]
Nucleotide change:

C17747230T

Amino acid change:

Q230term | sub-PA; Q230term | sub-PB

Reported amino acid change:

Q230term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Jang et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sub1/sub131 oocytes exhibit frequent monopolar and tripolar spindles as well as mono-orientation of homologous centromeres while they do not exhibit any failure to separate chromosomes or a defect in the movement of centromeres toward a pole present in the spindle as compared to controls.

      (Radford et al., 2012)

      Live-imaging analysis of metaphase I-arrested spindles in sub1 mutant oocytes reveals spindle instability; during the observation period an ectopic pole forms around the equatorial region in approximately half of the spindles that were initially bipolar. In most cases, the bipolarity is restored as this ectopic pole merges with one of the main poles. Among the spindles that show ectopic poles at the beginning of the observation, approximately half become bipolar before the end of the observation.

      The time between nuclear envelope breakdown and the first appearance of microtubules around the chromosomes is not significantly different in the mutant oocytes compared to wild type.

      (Colombié et al., 2008)

      Expression of subCT.Scer\UAS.P\T.T:Avic-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in sub1794/sub1 females results in sterility.

      Expression of subL6.Scer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in a sub1/+ background results in increased meiotic nondisjunction in females.

      (Jang et al., 2007)

      The percentage of cells in mitosis in sub1/sub131 mutant larval brains is approximately double that of wild-type.

      sub1/sub131 larval brains show an increased frequency of spindle assembly defects. These include frayed microtubules, unequal distribution of microtubules in the two half spindles, and disorganised or absent interpolar microtubules. 70% of mutant brains show disorganised metaphase, and 46% show lagging chromosomes at anaphase compared to 11% and 9% respectively in wild-type brains.

      (Cesario et al., 2006)

      14/17 sub1/sub131 oocytes have abnormal spindle organisation. Among the abnormal spindles, 3 are monopolar, 9 are tripolar and 2 have other defects such as fraying of microtubules.

      (Jang et al., 2005)

      Heterozygous females with mama1 are fertile.

      (Doane, 1996)

      Eggs derived from homozygous females show no visible sign of embryonic development when observed under transmitted light in a stereo microscope. After 4-6 hours after egg deposition the originally unformly dense (like wild type) yolk mass starts to disintegrate into a network of darker and lighter yolk droplets. The defect may be in fertilisation or very early in embryonic development.

      (Schupbach and Wieschaus, 1989)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      sub131/sub1 has brain | larval stage phenotype, enhanceable by polo16-1/polo[+]

      (Cesario et al., 2006)

      sub1 has spindle & oocyte phenotype, enhanceable by taccstella592

      (Jang et al., 2005)
      Suppressed by
      Statement
      Reference

      sub131/sub1 has brain | larval stage phenotype, suppressible by ncd1/ncd1

      (Cesario et al., 2006)
      Enhancer of
      Statement
      Reference

      sub1 is an enhancer of spindle & oocyte phenotype of taccstella592

      (Jang et al., 2005)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      sub1 ; taccstella592 double mutant females have severe spindle formation defects in the oocyte, more dramatic than either single mutant. There are often multiple bundles of microtubules in the double mutants, some associating with chromosomes, although most bundles of microtubules are randomly organised.

      (Jang et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (14)