FlyBase Allele Report: Dmel\CycA[hs.PK]

General Information

Symbol

Dmel\CycA^hs.PK

Species

D. melanogaster

Name

heat shock construct of Knoblich

FlyBase ID

FBal0029120

Feature type

allele

Associated gene

Dmel\CycA

Associated Insertion(s)

Carried in Construct

P{hs-CycA}

Also Known As

hs-cycA, HS-cyclin A

Key Links

Transgenic product class

cDNA

(Lehner et al., 1991)

wild_type

(Lehner et al., 1991)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lehner et al., 1991)

wild_type

(Lehner et al., 1991)

Mutagen

in vitro construct

(Sprenger et al., 1997, Knoblich and Lehner, 1993, Lehner et al., 1991)

Progenitor genotype

Carried in construct

P{hs-CycA}

Cytology

Description

NcoI-NheI fragment of CycA cDNA is driven from an Hsp70 promoter.

(Lehner et al., 1991)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

CycA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Ohlmeyer and Schupbach, 2003, Lehner et al., 1991)

increased occurrence of cell division | embryonic stage

(Sprenger et al., 1997)

increased occurrence of cell division | third instar larval stage

(Dong et al., 1997)

wild-type

(Knoblich and Lehner, 1993)

Phenotype Manifest In

amnioserosa

(Lehner et al., 1991)

egg chamber

(Ohlmeyer and Schupbach, 2003)

egg chamber & cell

(Lilly et al., 2000)

egg chamber & mitotic cell cycle

(Lilly et al., 2000)

embryonic epidermis

(Sprenger et al., 1997)

eye

(Dong et al., 1997)

eye disc | third instar larval stage

(Dong et al., 1997)

eye disc morphogenetic furrow

(Thomas et al., 1997)

S phase | ectopic

(Thomas et al., 1997)

Detailed Description

Statement

Reference

Overexpression of CycA^hs.PK results in an extra mitotic division in 4% of egg chambers.

(Ohlmeyer and Schupbach, 2003)

Unlike wild-type, CycA^C8LR1 mutants do not show an increased proportion of metaphase figures after exposure to radiation.

(Su and Jaklevic, 2001)

When CycA^hs.PK is over-expressed in adult females germline cysts with an aberrant number of cells are produced. Five days after heat-shock treatment cysts containing 32 instead of 16 cells are seen in 3% of egg chambers. The oocyte contains 5 ring canals (as compared to 4 in wild-type). Very rarely, cysts with an intermediate number of cells (in between 16 and 32) are seen.

(Lilly et al., 2000)

Heat induced expression during third larval instar results in a rough eye phenotype, this correlates with the induction of many cells into S phase in eye discs. Wild type Rca1 cannot suppress entry into S phase.

(Dong et al., 1997)

Expression of CycA^hs.PK in embryos using a 30 minute heat shock induces premature S phase in epidermal cells and endocycling tissues. Expression of CycA^hs.PK using a 10 minute heat shock does not induce S phase in epidermal cells of wild-type embryos carrying a single copy of CycA^hs.PK.

(Sprenger et al., 1997)

Ectopic S phase entry in the morphogenetic furrow. rux^hs.PT inhibits the ectopic S phase entry at the morphogenetic furrow but not in anterior or posterior cells.

(Thomas et al., 1997)

Heat induced expression induces entry into S phase in cells of the amnioserosa.

(Lehner et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CycA^hs.PK is an enhancer of abnormal mitotic cell cycle | maternal effect phenotype of png¹⁰⁵⁸/png³³¹⁸

(Lee et al., 2001)

Other

Statement

Reference

CycA^hs.PK, grp⁰⁶⁰³⁴ has lethal phenotype

(Su et al., 1999)

Cdk1^AF.hs, CycA^hs.PK has increased occurrence of cell division | embryonic stage phenotype

(Su et al., 1998, Sprenger et al., 1997)

CycA^hs.PK, CycE^AR95 has increased occurrence of cell division | embryonic stage phenotype

(Sprenger et al., 1997)

CycA^hs.PK, rux^unspecified has increased occurrence of cell division | embryonic stage phenotype

(Sprenger et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

CycA^hs.PK has mitotic cell cycle phenotype, suppressible by Cdk1^AF.hs/rux^hs.PT

(Foley et al., 1999)

Cdk1^AF.hs, CycA^hs.PK has mitotic cell cycle phenotype, suppressible by rux^hs.PT

(Foley et al., 1999)

Other

Statement

Reference

Cdk1^AF.hs, CycA^hs.PK has mitotic cell cycle phenotype

(Su et al., 2000, Foley et al., 1999)

Cdk1^AF.hs, CycA^hs.PK has mitotic domain 4 phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycA^hs.PK has primordial germ cell phenotype

(Su et al., 1998)

Cdk1^AF.hs, CycA^hs.PK has embryonic epidermis phenotype

(Sprenger et al., 1997)

CycA^hs.PK, CycE^AR95 has embryonic epidermis phenotype

(Sprenger et al., 1997)

CycA^hs.PK, rux^unspecified has embryonic epidermis phenotype

(Sprenger et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Many cells of heatshocked embryos carrying cdc2^AF.hs and CycA^hs.PK fail to delay mitosis after irradiation.

(Su et al., 2000)

Simultaneous over-expression of CycA^hs.PK and cdc2^AF.hs is sufficient to induce mitosis throughout the entire embryo. This phenotype is suppressed by the addition of rux^hs.PT.

(Foley et al., 1999)

A single copy of CycA^hs.PK is sufficient to cause homozygous lethality in grp⁰⁶⁰³⁴ embryos, even without heatshock.

(Su et al., 1999)

Heat induced coexpression of CycA^hs.PK and Cdk1^AF.hs induces proliferation in quiescent pole cells. Ectopic mitoses are followed by cell division in pole cells. Heat induced coexpression of CycA^hs.PK and Cdk1^hs.PS fails to induce proliferation in quiescent pole cells.

(Su et al., 1998)

Expression of CycA^hs.PK using a 10 minute heat shock induces S phase in epidermal cells of rux^unspecified embryos carrying a single copy of CycA^hs.PK. S phase is widely induced in the internal tissues that endocycle in wild-type embryos in CycE^AR95 embryos carrying two copies of CycA^hs.PK, within 1 hour of heat shock. Induction of S phase is also seen in epidermal cells, although not until 180 minutes after heat shock. The efficiency of S phase induction in embryos is dramatically increased if CycA^hs.PK is co-expressed with cdc2^AF.hs using heat shock.

(Sprenger et al., 1997)

CycA^hs.PK flies exhibit ectopic S phase entry in the morphogenetic furrow, rux^hs.PT inhibits the ectopic S phase entry at the morphogenetic furrow but not in anterior or posterior cells.

(Thomas et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

CycA^hs.PK rescues CycA³

(Knoblich and Lehner, 1993)

Comments

Images (0)

Mutant

Wild-type