Thomas, B.J., Zavitz, K.H., Dong, X., Lane, M.E., Weigmann, K., Finley, R.L., Brent, R., Lehner, C.F., Zipursky, S.L. (1997). roughex down-regulates G2 cyclins in G1. Genes Dev. 11(10): 1289--1298.
FlyBase ID
FBrf0093752
Publication Type
Research paper
Abstract
Cell cycle arrest in G1 at the onset of patterning in the Drosophila eye is mediated by roughex. In roughex mutants, cells accumulate Cyclin A protein in early G1 and progress into S phase precociously. When Roughex is overexpressed in S/G2 cells, Cyclin A is mislocalized to the nucleus and degraded, preventing mitosis. Whereas Roughex inhibits Cyclin A accumulation, Cyclin E down-regulates Roughex protein in vivo. Roughex binds to Cyclin E and is a substrate for a Cyclin E-Cdk complex in vitro. These data argue that Roughex inhibits Cyclin A accumulation in early G1 by targeting Cyclin A for destruction. In late G1, Roughex is destabilized in a Cyclin E-dependent process, releasing Cyclin A for its role in S/G2.
