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Thomas, B.J., Zavitz, K.H., Dong, X., Lane, M.E., Weigmann, K., Finley, R.L., Brent, R., Lehner, C.F., Zipursky, S.L. (1997). roughex down-regulates G2 cyclins in G1.  Genes Dev. 11(10): 1289--1298.
FlyBase ID
FBrf0093752
Publication Type
Research paper
Abstract

Cell cycle arrest in G1 at the onset of patterning in the Drosophila eye is mediated by roughex. In roughex mutants, cells accumulate Cyclin A protein in early G1 and progress into S phase precociously. When Roughex is overexpressed in S/G2 cells, Cyclin A is mislocalized to the nucleus and degraded, preventing mitosis. Whereas Roughex inhibits Cyclin A accumulation, Cyclin E down-regulates Roughex protein in vivo. Roughex binds to Cyclin E and is a substrate for a Cyclin E-Cdk complex in vitro. These data argue that Roughex inhibits Cyclin A accumulation in early G1 by targeting Cyclin A for destruction. In late G1, Roughex is destabilized in a Cyclin E-dependent process, releasing Cyclin A for its role in S/G2.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Alleles (10)
    Genes (14)
    Insertions (1)
    Experimental Tools (3)
    Transgenic Constructs (7)