FlyBase Allele Report: Dmel\bgcn[QS2]

General Information

Symbol

Dmel\bgcn^QS2

Species

D. melanogaster

Name

FlyBase ID

FBal0030625

Feature type

allele

Associated gene

Dmel\bgcn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gonczy et al., 1997)

Mutagen

ethyl methanesulfonate

(Gonczy et al., 1997, Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: W760term.

(Ohlstein et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,855,044..23,855,044 [-]

Nucleotide change:

G23855044A

Amino acid change:

W760term | bgcn-PA; W755term | bgcn-PB

Reported amino acid change:

W760term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ohlstein et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous bgcn^QS2 mutants arrest at the gonial stage, resulting in a large over-proliferation of transit amplifying (TA) gonia with branched fusomes and the absence of both spermatocyte and spermatid stages. bgcn^QS2 mutant testes exhibit no expansion of dot fusome-containing germline stem cells (GSC) or gonialblasts.

(Terry et al., 2006)

bgcn^QS2 homozygous females have ovaries with 'stem cell tumors' consisting of large numbers of undifferentiated germ cells.

(Gilboa et al., 2003)

Homozygotes have germline tumours in their gonads.

(Ohlstein et al., 2000)

Fate and proliferation of somatic cyst and hub cells is not affected in mutants testes. Mutant testes are not sexually transformed. Mutant clones induced in the cysts of male testes produce cysts with many more than 16 small germ cells, each having the morphological features of amplifying germ cells. Germ cells accumulating in the testes have a mixed identity, simultaneously retaining some aspects of stem cell fate while expressing markers of amplifying spermatogonial and primary spermatogonial cell fates. Distribution of both fusome and ring canal components indicates many mutant germ cells within a cyst are interconnected as a result of incomplete cytokinesis during the divisions leading to overproliferation. Most germ cells within a cyst undergo S and M phases in near-synchrony.

(Gonczy et al., 1997)

Tumorous ovaries. Oogenesis is normal in homozygotes when in combination with otu¹ heterozygotes.

(Partanen et al., 1991)

ovarian tumors with many small, undifferentiated cells in ovaries affected early stages of oogenesis (before yolk uptake).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

bgcn^QS2 has hyperplasia phenotype, suppressible by zpg^unspecified/zpg^unspecified

(Gilboa et al., 2003)

Enhancer of

Statement

Reference

bgcn[+]/bgcn^QS2 is an enhancer of increased cell number | recessive phenotype of tut⁴

(Chen et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

bgcn^QS2 has female germline stem cell phenotype, suppressible by zpg^unspecified/zpg^unspecified

(Gilboa et al., 2003)

Enhancer of

Statement

Reference

bgcn[+]/bgcn^QS2 is an enhancer of testis phenotype of tut⁴

(Chen et al., 2014)

bgcn[+]/bgcn^QS2 is an enhancer of spermatogonium phenotype of tut⁴

(Chen et al., 2014)

Other

Statement

Reference

bam^Δ86, bgcn^QS2 has germline cell phenotype

(Gonczy et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Introducing bgcn²⁰⁰⁹³/+ or bgcn^QS2/+ into the tut⁴ background results in the majority of germ cells continuing to divide but unable to differentiate beyond spermatogonial stage.

(Chen et al., 2014)

The 'stem cell tumor' phenotype seen in the ovaries of bgcn^QS2 homozygous females is suppressed by zpg^unspecified/zpg^unspecified.

(Gilboa et al., 2003)

bgcn^QS2; bam^Δ86 double mutant germ cells also are interconnected and undergo S phase in synchrony. The double mutant germ cells, just like germ cells in either single mutant behave as amplifying primary or secondary spermatogonial cells, and not as stem cells, indicating that bam and bgcn are not partially redundant with each other.

(Gonczy et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Penetrance of interaction with otu¹ is 73%.

(Partanen et al., 1991)

Dominant suppressors of the sterility phenotype of bgcn^QS2 have properties similar to those of Su(var)'s, also suppressing variegation of In(1)w^m4.

(Mahowald and Wei, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

bcgn^-

(Sturgill et al., 2007)

bgcn^QS2

(Shan et al., 2017, Chen et al., 2014, Terry et al., 2006)

bgcn^qs2

(Lin et al., 1994)

fs(2)eo6¹

pep^QS2

(Partanen et al., 1991, Schupbach and Wieschaus, 1991)

Name Synonyms

Secondary FlyBase IDs

FBal0004598

References (15)

Report Sections

Open Close