Amino acid replacement: G3601S. Mutation is in the conserved SET domain.
Amino acid replacement: G3601S. Residue is in the highly conserved C-terminal tromo domain.
G14265451A
G3601S | trx-PA; G3233S | trx-PB; G3233S | trx-PC; G3601S | trx-PD; G3233S | trx-PE
27% of type II neuroblast clones in homozygous clones in the larval brain adopt a type I neuroblast identity (as determined by marker expression and the direct generation of ganglion mother cells).
1.3% of heterozygous adults show at least one transformation phenotype. Hemizygotes are pupal lethal, homozygotes are viable, showing a large number of homeotic transformations. trxZ11/Df(3R)red-P52 hemizygotes show phenotypes that suggest decreased Scr function (transformation of T1 structures to a T2 identity), decreased Ubx function (transformation of T3 to T2), decreased abd-A function (transformation of A2-A7 to A1) and decreased Abd-B function (transformation of posterior abdominal segments to more anterior abdominal identities). Scr group transformations include the development of mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the prothoracic tibia, reduced numbers of sex comb teeth (in males), development of sternopleural bristles or katepisternal bristles on the T1 segment. Ubx group transformations include haltere to wing, metathoracic laterotergite to scutum, the development of T3 sternopleural bristles, mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the metathoracic tibia. abd-A group transformations include, the development of A1 type bristles on abdominal tergites 2 to 3. Abd-B group transformations include, the pigmentation of abdominal tergites 5 to 6 in males, and enlargement of abdominal tergite 7. trx3/trxZ11 adults occasionally developed small heads, anterior thoracic segments, abnormal antennae with reduced aristae and reduced or absent maxillary palps. They also often develop A1 tergites with dark pigmentation and large Uab-like bristles at their posterior borders. The flies have patches of small bristles in the A2 and A3 tergites that are normally only found in the A1 tergite. Transformation of ventral T1 to ventral T2 is seen, and sternopleural bristles are present on T1.
Partial complementation between hypomorphs produced phenotypes that were weaker than either of the hemizygous or homozygous phenotypes, either rescuing lethality or producing a weaker homeotic transformation phenotype. Transformations range from an average 0.27 to 16.39 transformations/fly (males were scored for a possible 29 transformations, females were scored for a possible 26 transformations) in different allelic combinations.
The following alleles form a series of progressively increasing penetrance and expressivity of phenotype when in transheterozygous combination with trx1 : trxM17 > trxZ32 > trxZ16 > trxZ11 > trxM18 = trxJY16 > trxE3.