FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Met3
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General Information
Symbol
Dmel\Met3
Species
D. melanogaster
Name
FlyBase ID
FBal0033990
Feature type
allele
Associated gene
Dmel\Met
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Wilson et al., 2006)
Mutagen
ethyl methanesulfonate
(Ashok et al., 1998, Restifo and Wilson, 1998, Minkoff and Wilson, 1992, Shemshedini and Wilson, 1990)
Progenitor genotype
Cytology
Description

Amino acid replacement: A77T.

(Wilson et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:11,617,096..11,617,096 [+]
Nucleotide change:

G11617096A

Amino acid change:

A77T | Met-PA; A77T | Met-PB

Reported amino acid change:

A77T

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wilson et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutants show strong resistance to methoprene, juvenile hormone III or methyl farnesoate applied topically compared to control flies.

      (Zhang et al., 2010)

      Rst(1)JH3 flies die in response to concentrations of methoprene that wild-type flies can survive.

      (Wilson et al., 2006)

      Homozygotes show increased resistance to methoprene compared to controls.

      16% of mutant flies have a small number of defective ommatidia in the posterior quarter of the eye.

      (Wilson et al., 2006)

      Homozygotes do not show subesophageal ganglion-thoracic ganglion (SEG-TG) fusion defects when fed 100μl methoprene, in contrast to wild-type flies, which show severe SEG-TG fusion defects when fed 100μl methoprene.

      (Restifo and Wilson, 1998)

      Flies carrying Rst(1)JH3 have a competitive disadvantage compared to wild-type flies. Females have reduced fecundity compared to wild-type.

      (Minkoff and Wilson, 1992)

      Strong allele. Rst(1)JH3 flies have an approximately 10-fold lower binding affinity for juvenile hormone III than Rst(1)JH+ flies.

      (Shemshedini and Wilson, 1990)

      slightly stronger than Rst(1)JH1

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Met3 has chemical sensitive phenotype, non-suppressible by br1/br1

      (Wilson et al., 2006)
      Enhancer of
      Statement
      Reference

      Met3/Met3 is an enhancer of lethal - all die during P-stage phenotype of brrbp-2/brnpr-3

      (Wilson et al., 2006)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Met3/Met3 is an enhancer of ovary phenotype of br1

      (Wilson et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality is enhanced in brrbp-2/brnpr-3 Rst(1)JH3/Rst(1)JH3 double mutants compared to brrbp-2/brnpr-3 mutants, with no flies surviving beyond the pupal stage. br1/Y, Rst(1)JH3/Y hemizygotes show good levels of survival. br1, Rst(1)JH3 flies show the same sensitivity to methoprene as br1 single mutant flies. br1, Rst(1)JH3 double mutant escapers show significantly reduced levels of oogenesis compared to br1 single mutants

      (Wilson et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Met27

      (Wilson and Ashok, 1998)
      Rescued by

      Met3 is rescued by Meths.PA

      (Ashok et al., 1998)

      Met3 is rescued by Met+tSt-H.hs

      (Ashok et al., 1998)
      Not rescued by

      Met3 is not rescued by MetSt-X.hs

      (Ashok et al., 1998)

      Met3 is not rescued by MetK-H.hs

      (Ashok et al., 1998)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Tom Wilson.

      Comments
      Comments

      The fitness component(s) responsible for the competitive disadvantage of flies carrying mutant alleles of Rst(1)JH have been analysed in Rst(1)JH1, Rst(1)JH2, Rst(1)JH3, Rst(1)JHD29 and Rst(1)JHN6 flies. Small but significant differences were found between the pooled Rst(1)JH alleles and wild-type for pupal developmental time, pupal mortality, and early adult fecundity. These differences results in a large competitive disadvantage.

      (Minkoff and Wilson, 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (10)