FlyBase Allele Report: Dmel\dpp[Sal20]

General Information

Symbol

Dmel\dpp^Sal20

Species

D. melanogaster

Name

FlyBase ID

FBal0034420

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{dpp-Sal20}

Also Known As

P20

Key Links

Genomic Maps

JBrowse

Transgenic product class

genomic_DNA

(Hursh et al., 1993)

wild_type

(Hursh et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Hursh et al., 1993)

wild_type

(Hursh et al., 1993)

Mutagen

in vitro construct

(Held and Heup, 1996, Padgett and Gelbart, 1995.3.25, Hursh et al., 1993, Wharton et al., 1993, Ferguson and Anderson, 1992)

Progenitor genotype

Carried in construct

P{dpp-Sal20}

Cytology

Description

dpp rescue construct: fragment of dpp from coordinates 71.2 to 92 of dpp region, i.e. missing 5' most alternative non-coding exon, but including sequences from the shv region of dpp.

(Hursh et al., 1993)

20kb fragment including the dpp transcription unit and surrounding regulatory sequences.

(Ferguson and Anderson, 1992)

Allele components

Component

Use(s)

Regulatory region(s)

dpp

Encoded product / tool

dpp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

rescue_region

2L:2,442,454..2,463,173 [+]

Linked to:

SalI-SalI restriction fragment

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

increased cell death, with Df(2L)DTD2/dpp^TgR46.1

(Stultz et al., 2006)

visible, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

visible, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007, Stultz et al., 2006)

wild-type

(Nicholls and Gelbart, 1998)

Phenotype Manifest In

adult head | ventral, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007)

amnioserosa

(Mason et al., 1997)

eye, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

eye, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007, Stultz et al., 2006)

eye-antennal disc, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

eye-antennal disc, with Df(2L)DTD2/dpp^TgR46.1

(Stultz et al., 2006)

gena, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

gena, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007, Stultz et al., 2006)

maxillary palp, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

maxillary palp, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007, Stultz et al., 2006)

ommatidium, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

ommatidium, with Df(2L)DTD2/dpp^TgR46.1

(Stultz et al., 2006)

rostral membrane | anterior, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

rostral membrane | anterior, with Df(2L)DTD2/dpp^TgR46.1

(Stultz et al., 2006)

vibrissal bristle, with Df(2L)DTD2, dpp^TgR46.1

(Stultz et al., 2006)

vibrissal bristle, with Df(2L)DTD2/dpp^TgR46.1

(Lee et al., 2007, Stultz et al., 2006)

Detailed Description

Statement

Reference

In dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 flies the ventral head is disrupted, the eye is round rather than oval, the sensory vibrissae on the ventral side of the eye are bunched, the maxillary palps are missing, reduced or duplicated, and gena tissue appears to be missing.

(Lee et al., 2007)

dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 flies show head capsule defects. The sensory vibrissae on the ventral side of the head are disrupted, with vibrissae missing at the posterior end, and bunched or duplicated at the anterior end. The maxillary palps are either missing, reduced or are sometimes duplicated. In extreme cases, both the anterior rostral membrane and gena tissue are greatly diminished. The eyes are round instead of oval and are small due to a small, but statistically significant reduction in ommatidia number.

Third star imaginal discs appear normal in size and shape in dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 larvae.

Third star dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 eye-antennal discs show increased cell death, particularly in the disc proper.

(Stultz et al., 2006)

Embryos carrying two copies of dpp^Sal20 produce approximately twice the normal number of amnioserosa cells. This phenotype is not altered if the embryos are also carrying two copies of tsg^twi.PM.

(Mason et al., 1997)

Covers functions mediated by the dpp^Hin and dpp^shv region.

(Padgett and Gelbart, 1995.3.25)

dpp sequences remaining in this allele are sufficient for rescue the larval lethality of dpp null alleles.

(Hursh et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

dpp^Sal20, dpp^hr27 has phenotype, non-enhanceable by Df(3L)66C-G28/+

(Nicholls and Gelbart, 1998)

dpp^Sal20, dpp^hr56 has phenotype, non-enhanceable by Df(3L)66C-G28/+

(Nicholls and Gelbart, 1998)

dpp^Sal20, dpp^hr4 has phenotype, non-enhanceable by Df(3L)66C-G28/+

(Nicholls and Gelbart, 1998)

Suppressor of

Statement

Reference

dpp^Sal20 is a suppressor of embryo phenotype of sax^unspecified

(Xie et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

Rescues embryogenesis defects of embryos from sax mutant mothers.

(Xie et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

dpp^Sal20 rescues dpp^s6/dpp^H46

(Stultz et al., 2005)

dpp^Sal20 rescues Df(2L)DTD2/dpp^s6

(Stultz et al., 2005)

dpp^Sal20 rescues dpp^hr4/Df(2R)Pcl11B

(Nicholls and Gelbart, 1998)

Partially rescues

dpp^Sal20 partially rescues dpp^H46/dpp^s11

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s11

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^s20/dpp^H46

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s20

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^s7/dpp^H46

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s7

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^H46/dpp^s3

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s3

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^s28/dpp^H46

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s28

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^s26/dpp^H46

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^s26/Df(2L)DTD2

(Stultz et al., 2005)

dpp^Sal20 partially rescues Df(2L)DTD2/dpp^s-hc1

(Stultz et al., 2005)

dpp^Sal20 partially rescues dpp^unspecified

(Gelbart, 1997.12.4)

dpp^Sal20 partially rescues dpp^unspecified

(Newfeld et al., 1997)

dpp^Sal20 partially rescues dpp^unspecified

(Padgett and Gelbart, 1995.3.25)

dpp^Sal20 partially rescues dpp^H46

(Hursh et al., 1993)

Comments

Expression of dpp^Scer\UAS.cNa under the control of Scer\GAL4^dpp.s-hc at 29^oC causes an almost complete rescue of the head capsule defects of dpp^TgR46.1/Df(2L)DTD2, dpp^Sal20 mutants. Expression at 27^oC is less effective and causes a 55% reduction in penetrance while expression at 25^oC results in a 22% reduction in penetrance.

(Stultz et al., 2006)

dpp^Sal20 rescues both the embryonic and shortvein region lethal phases, and in a null background, animals die during pupation with a dpp disk class V mutant phenotype.

(Gelbart, 1997.12.4)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

99042

P{dpp-Sal20}1

99041

y¹ w^*; P{dpp-Sal20}2, Df(2L)DTD2/In(2LR)Gla, wg^Gla-1 PPO1^Bc Egfr^E1

Notes on Origin

Discoverer

R. Padgett.

(Wharton et al., 1993)

Comments

dpp sequences remaining in this allele are sufficient for correct expression of dpp in parasegments 4 and 7 of the visceral mesoderm.

(Hursh et al., 1993)

Sufficient to rescue all Hin and shv functions.

(Newfeld et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpp^+t20

dpp^P20

(Lee et al., 2007)

dpp^Sal20

Name Synonyms

Secondary FlyBase IDs

FBal0031063

References (16)

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