FlyBase Allele Report: Dmel\RpS21[k16814]

General Information

Symbol

Dmel\RpS21^k16814

Species

D. melanogaster

Name

FlyBase ID

FBal0035096

Feature type

allele

Associated gene

Dmel\RpS21

Associated Insertion(s)

P{lacW}RpS21^k16814

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Torok et al., 1999)

Mutagen

P-element activity

(Roch et al., 1998, Torok et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{lacW}RpS21^k16814

Cytology

Description

P{lacW} insertion in the 5' untranslated region of the 0.4kb oho23B transcript.

(Torok et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | dominant

(Torok et al., 1999)

hyperplasia | recessive

(Torok et al., 1999)

lethal

(Torok et al., 1993)

lethal | recessive

(Spradling et al., 1999, Torok et al., 1999, Roch et al., 1998)

Minute

(Marygold et al., 2007)

visible | dominant

(Torok et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

The development of homozygotes is considerably delayed compared to controls; when their heterozygous siblings eclose from the pupal case, homozygous larvae are the size of young third instar larvae. The homozygous larvae reach a nearly mature size 10-15 days after egg laying and remain as third instar larvae for up to 3 weeks. They then undergo puparium formation, although eversion of the wing and leg discs is incomplete. Retraction of the epidermis occurs, leaving gas bubbles on both extremities of the pupal case, but head eversion does not occur. The imaginal discs are slightly larger than normal in 1/3 of homozygous larvae. The hematopoietic organs are much larger than normal (up to 50 times the normal size) in aged larvae, with hyperplasia occurring maximally in the first pair of lobes and gradually decreasing in the more posterior lobes. The hematopoietic organs retain a globular and compact structure. Melanotic masses are not seen. The number of circulating hemocytes is strongly reduced compared to wild type. Heterozygotes have a "Minute" phenotype of short, slender bristles, a small body size (approximately 12 to 20% smaller than wild type) and a 2 day delay in eclosion at 25^oC due to a delay in puparium formation. A partial cleft extending anteriorly from the scutoscutellar suture is seen on both sides of the scutum.

(Torok et al., 1999)

Lethality occurs during third instar larval or pupal stages. Mutants exhibit visible disc abnormalities: very small or no discs. Clones cannot be induced in wild type discs. In tergites, clones with normal size and frequency are recovered. Clones cannot be recovered in the wing disc.

(Roch et al., 1998)

Lethality occurs during pupal and pharate adult stages. Brain is of normal size but abnormal shape, discs are smaller than wild type with abnormal folding. Both brain and discs grow with age. Lymph glands retain lobular structure.

(Torok et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RpS21^k16814 has lethal | larval stage | recessive phenotype, enhanceable by sta²

(Torok et al., 1999)

RpS21^k16814 has visible | dominant phenotype, enhanceable by Dp(1;3)sta/sta²

(Torok et al., 1999)

RpS21^k16814 has visible | dominant phenotype, enhanceable by sta¹/sta²

(Torok et al., 1999)

RpS21^k16814 has visible | dominant phenotype, enhanceable by sta¹

(Torok et al., 1999)

Enhancer of

Statement

Reference

RpS21^k16814 is an enhancer of lethal | larval stage | recessive phenotype of sta²

(Torok et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

RpS21^k16814 has macrochaeta phenotype, enhanceable by Dp(1;3)sta/sta²

(Torok et al., 1999)

RpS21^k16814 has macrochaeta phenotype, enhanceable by sta¹/sta²

(Torok et al., 1999)

RpS21^k16814 has macrochaeta phenotype, enhanceable by sta¹

(Torok et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

sta²/Y ; oho23B^k16814/oho23B^k16814 animals die as first to second instar larvae. sta²/Y ; oho23B^k16814/+ and sta²/sta²; oho23B^k16814/+ animals die as first to second instar larvae. sta²/+ ; oho23B^k16814/oho23B^k16814 third instar larvae are larger in size and have much larger overgrown imaginal discs than oho23B^k16814/oho23B^k16814 larvae. The leg discs tend to fuse together and the hematopoietic organs are larger than in oho23B^k16814/oho23B^k16814 larvae. The sta²/+ ; oho23B^k16814/oho23B^k16814 third instar larvae are more active than oho23B^k16814/oho23B^k16814 third instar larvae. The bristle phenotype of oho23B^k16814/+ heterozygotes is enhanced by sta²; Dp(1;3)sta/+, sta¹/sta² or sta¹/+.

(Torok et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RpS21⁰³⁵⁷⁵

(BDGP Project Members, 1994-1999)

RpS21^k16804a

(BDGP Project Members, 1994-1999)

Rescued by

RpS21^k16814 is rescued by RpS21^+t2.8

(Torok et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

103045

y¹ w^67c23 ; P{lacW}RpS21^k16814 / CyO

Notes on Origin

Discoverer

Comments

P-element excision demonstrates the insertion to be the cause of lethality.

(Torok et al., 1993)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ009557

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

168/14

(Roch et al., 1998)

RpS21^k16814

l(2)k168-14

(Torok et al., 1999)

l(2)k16814

(Spradling et al., 1999)

l(2)k16814^k16814

oho23B^k16814

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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