FlyBase Allele Report: Dmel\sc[UAS.cHa]

General Information

Symbol

Dmel\sc^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hinz

FlyBase ID

FBal0035542

Feature type

allele

Associated gene

Dmel\sc

Associated Insertion(s)

Carried in Construct

P{UAS-sc.USC}

Also Known As

UAS-sc

Key Links

Transgenic product class

wild_type

(Hinz et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hinz et al., 1994)

Mutagen

in vitro construct

(Hinz et al., 1994)

Progenitor genotype

Carried in construct

P{UAS-sc.USC}

Cytology

Description

UASt regulatory sequences drive expression of sc.

(Hinz et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^bbg-C96

(Johnston and Edgar, 1998)

flightless, with Scer\GAL4^sr-md710

(Usui et al., 2004)

visible, with Scer\GAL4^ap-md544

(Nolo et al., 2000)

visible, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

visible, with Scer\GAL4^sca-C253

(Usui et al., 2004)

visible, with Scer\GAL4^sr-md710

(Usui et al., 2004)

Phenotype Manifest In

adult thorax & macrochaeta, with Scer\GAL4^ap-md544

(Nolo et al., 2000)

adult thorax & microchaeta, with Scer\GAL4^ap-md544

(Nolo et al., 2000)

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^sr-md710

(Usui et al., 2004)

haltere, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

haltere & macrochaeta, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

haltere sensillum | ectopic, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

macrochaeta & scutellum, with Scer\GAL4^455.2

(Hinz et al., 1994)

macrochaeta | ectopic, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

macrochaeta | ectopic, with Scer\GAL4^sr-md710

(Usui et al., 2004)

mesothoracic tergum & macrochaeta | ectopic, with Scer\GAL4^ap-md544

(Wheeler et al., 2003)

mesothoracic tergum & macrochaeta | ectopic, with Scer\GAL4^sca-C253

(Usui et al., 2004)

microchaeta, with Scer\GAL4^sr-md710

(Usui-Ishihara and Simpson, 2005)

wing, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

wing & macrochaeta, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

wing disc, with Scer\GAL4^bbg-C96

(Johnston and Edgar, 1998)

wing sensillum | ectopic, with Scer\GAL4^dpp.blk1

(Weatherbee et al., 1998)

Detailed Description

Statement

Reference

When sc^Scer\UAS.cHa is driven by Scer\GAL4^sr-md710 animals have large bristles that have longer shafts than those of normal microchaetes. These bristle display complex projections with a large number of branches. When sc^Scer\UAS.cHa is driven by Scer\GAL4^pnr-MD237 animals have very long bristles, some of which are as long as macrochaetae. These often display an anterior dorsocentral (aDC) like projection pattern or when close to the extant posterior DC (pDC) macrochaete, a pDC-like projection. They often have more side branches.

(Usui-Ishihara and Simpson, 2005)

Expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^sca-C253 induces ectopic macrochaetae on the notum. Expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^sr-md710 results in ectopic macrochaetae in the domain of sr expression. The longitudinal flight muscles of these adults are very much thinner and more numerous than normal and the animals cannot fly, although they can walk.

(Usui et al., 2004)

sc^Scer\UAS.cHa; Scer\GAL4^ap-md544 adults have ectopic bristles in their nota.

(Wheeler et al., 2003)

Expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 causes few or no extra bristles. Expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 results in numerous extra thoracic bristles.

(Nolo et al., 2000)

When expression is driven by Scer\GAL4^C96 throughout the ZNC in the developing wing, G2 arrest occurs in both posterior and anterior compartments.

(Johnston and Edgar, 1998)

Flies expressing sc^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 produce ectopic sensory structures; ectopic bristles resembling double row margin bristles are induced along the distal anterior-posterior boundary of the wing, and campaniform sensilla are induced more proximally on the wing. Ectopic capitellar sensillae are induced on the proximal haltere, and ectopic bristles resembling double row wing margin bristles are induced on the distal haltere.

(Weatherbee et al., 1998)

Ectopic bristles develop on the scutellum.

(Hinz et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^sca-C253, sc^UAS.cHa has visible phenotype, suppressible by sr^b.UAS/Scer\GAL4^sca-C253

(Usui et al., 2004)

Scer\GAL4^ap-md544, sc^UAS.cHa has visible phenotype, suppressible | somatic clone by sens^E2

(Nolo et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/sc^UAS.cHa is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, sc^UAS.cHa, sens^UAS.cNa has visible phenotype

(Nolo et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sca-C253, sc^UAS.cHa has mesothoracic tergum & macrochaeta | ectopic phenotype, suppressible by sr^b.UAS/Scer\GAL4^sca-C253

(Usui et al., 2004)

Scer\GAL4^ap-md544, sc^UAS.cHa has adult thorax & macrochaeta phenotype, suppressible | somatic clone by sens^E2

(Nolo et al., 2000)

Scer\GAL4^ap-md544, sc^UAS.cHa has adult thorax & microchaeta phenotype, suppressible | somatic clone by sens^E2

(Nolo et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/sc^UAS.cHa is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

Scer\GAL4^GMR.PF/sc^UAS.cHa is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

Scer\GAL4^dpp.blk1/sc^UAS.cHa is a suppressor | partially of eye disc morphogenetic furrow phenotype of Scer\GAL4^dpp.blk1, wg^wg.3'UTR.UAS

(Cadigan et al., 2002)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, sc^UAS.cHa, sens^UAS.cNa has chaeta | ectopic phenotype

(Nolo et al., 2000)

Scer\GAL4^dpp.blk1, sc^UAS.cHa, sens^UAS.cNa has adult head | dorsal & chaeta | ectopic phenotype

(Nolo et al., 2000)

Scer\GAL4^dpp.blk1, sc^UAS.cHa, sens^UAS.cNa has adult head | ventral & chaeta | ectopic phenotype

(Nolo et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sr^b.Scer\UAS removes most of the ectopic bristles on the notum caused by expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^sca-C253 and also removes many of the extant ones.

(Usui et al., 2004)

Co-expression of sc^Scer\UAS.cHa does not significantly alter the small eye phenotype caused by expression of wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^GMR.PF, but does fully suppress the loss of interommatidial bristles seen in these flies. Co-expression of sc^Scer\UAS.cHa restores some progression of the morphogenetic furrow in animals expressing wg^{wg.3'UTR.Scer\UAS} under the control of Scer\GAL4^dpp.blk1.

(Cadigan et al., 2002)

Expression of both sens^Scer\UAS.cNa and sc^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 causes numerous extra bristles to appear, including in areas where either expressed alone does not give rise to ectopic bristles, such as dorsal and ventral portions of the head. Clones of sens^E2 in the thorax suppress the extra micro- and macrochaetae- produced by expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544.

(Nolo et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^sca-4512/sc^UAS.cHa rescues In(1)y^3PLsc^8R

(Skeath and Doe, 1996)

Partially rescues

Scer\GAL4^sca-109-68/sc^UAS.cHa partially rescues In(1)y^3PLsc^8R

(Wheeler et al., 2003)

Comments

sc^Scer\UAS.cHa; Scer\GAL4^sca-109-68 rescues the reduction in MP2 neuroblast formation seen in In(1)y^3PLsc^8R homozygous embryos.

(Wheeler et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51672

y¹ w^*; P{UAS-sc.USC}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sc^Scer\UAS.cHa

sc^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hinz

Secondary FlyBase IDs

References (14)

Report Sections

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