FlyBase Allele Report: Dmel\Hrb27C[k16203]

General Information

Symbol

Dmel\Hrb27C^k16203

Species

D. melanogaster

Name

FlyBase ID

FBal0044462

Feature type

allele

Associated gene

Dmel\Hrb27C

Associated Insertion(s)

P{lacW}Hrb27C^k16203

Carried in Construct

Also Known As

hrp48^k16203, l(2)k16203

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hammond et al., 1997)

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}Hrb27C^k16203

Cytology

Description

P{lacW} insertion 2.2kb from the Hrb27C coding sequence.

(Hammond et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage (with Hrb27C^k10413)

(Bruckert et al., 2015)

female semi-sterile | germline clone

(Yano et al., 2004)

female sterile (with Hrb27C^5A2-6)

(Huynh et al., 2004)

lethal

(Goodrich et al., 2004)

lethal (with Df(2L)wee^wo5)

(Yano et al., 2004)

lethal (with Hrb27C^7E7-18)

(Huynh et al., 2004)

lethal (with Hrb27C^10B2-9)

(Huynh et al., 2004)

partially lethal (with Hrb27C^k10413)

(Bruckert et al., 2015)

partially lethal - majority die

(Hammond et al., 1997)

partially lethal - majority die (with Hrb27C^k10413)

(Yano et al., 2004)

visible

(Hammond et al., 1997)

Phenotype Manifest In

egg (with Hrb27C¹⁰²⁸⁰)

(Goodrich et al., 2004)

egg | germline clone

(Yano et al., 2004)

egg chorion (with Hrb27C¹⁰²⁸⁰)

(Goodrich et al., 2004)

lobe system of adult mushroom body (with Hrb27C^k10413)

(Bruckert et al., 2015)

ommatidium | female

(Hammond et al., 1997)

oocyte & cytoskeleton | germ-line clone

(Yano et al., 2004)

posterior supraalar bristle

(Hammond et al., 1997)

Detailed Description

Statement

Reference

Hrb27C^k10413/Hrb27C^k16203 transheterozygous flies frequently have Mushroom Bodies (MB) with α and β lobes of unequal width (asymmetric), truncated α lobes or MBs lacking either the α or the β lobes, when compared to controls. They also exhibit abnormal overextension of dorsal lobes when compared to controls.

(Bruckert et al., 2015)

<3% of eggs laid by Hrb27C¹⁰²⁸⁰/Hrb27C^k16203 females exhibit any degree of dorsalisation.

(Goodrich et al., 2004)

Germline clones of Hrb27C^k16203 develop into egg chambers and laid eggs, although many of the eggs have a shape characteristic of dumping defects during the latest stages of oogenesis and are not fertilized. The organisation and polarity of the oocyte cytoskeleton is somewhat abberant in these germline clones. Of the eggs derived from these germ-line clones that are fertilised only 3% develop into larvae. Around half of unhatched eggs form embryos with wild-type cuticle, while 18% have fewer than 6 segments and 15% show head defects consistent with posterior patterning activity at the anterior.

(Yano et al., 2004)

Small eye phenotype is observed in the majority of homozygous females, the posterior supraalar macrochaetae is missing in most males and females.

(Hammond et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Hrb27C¹⁰²⁸⁰/Hrb27C^k16203 is an enhancer of egg chorion phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C¹⁰²⁸⁰/Hrb27C^k16203 is an enhancer of dorsal appendage phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C¹⁰²⁸⁰/Hrb27C^k16203 is an enhancer of egg operculum phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C¹⁰²⁸⁰/Hrb27C^k16203 is an enhancer of egg phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C[+]/Hrb27C^k16203 is an enhancer of embryonic/first instar larval cuticle & embryonic head phenotype of Scer\GAL4^{VP16.nanos.UTR}, osk^3'UTR.UASp

(Yano et al., 2004)

Other

Statement

Reference

Hrb27C¹⁰²⁸⁰/Hrb27C^k16203, sqd¹/sqd^K12 has nurse cell & nuclear chromosome phenotype

(Goodrich et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Dorsalisation of the eggs laid by sqd¹/sqd^K12 transheterozygotes is enhanced by Hrb27C¹⁰²⁸⁰/Hrb27C^k16203, so that 100% show some degree of dorsalisation.

(Goodrich et al., 2004)

The penetrance and severity of embryonic cuticle patterning defects in osk^{3'UTR.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos is enhanced by Hrb27C^k16203/+.

(Yano et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hrb27C^7E7-18

(Huynh et al., 2004)

Hrb27C^10B2-9

(Huynh et al., 2004)

Hrb27C^rF680

(BDGP Project Members, 1994-1999)

Partially rescued by

Hrb27C^k16203 is partially rescued by Hrb27C^Act5C.PH

(Hammond et al., 1997)

Hrb27C^k16203 is partially rescued by Hrb27C^hs.PH

(Hammond et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

I. Kiss.

Comments

Allelic series: Hrb27C^k02814 > Hrb27C^k16203 > Hrb27C⁰²⁶⁴⁷

(Yano et al., 2004)

Somatic P\T IVS3 splicing from a reporter transgene, P\T^{IVS3.Hsp83.T:Ecol\lacZ}, is activated in Hrb27C^k16203 mutants.

(Hammond et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Hrb27C^k16203

Hrp48^K16203

(Mhlanga et al., 2009)

hrb27C^k16303

(Goodrich et al., 2004)

hrp48⁴

(Hammond et al., 1997)

hrp48^k16203

(Bruckert et al., 2015, Yano et al., 2004)

l(2)02647^k16203

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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