FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ttkp69.UAS
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General Information
Symbol
Dmel\ttkp69.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0060851
Feature type
allele
Associated gene
Dmel\ttk
Associated Insertion(s)
Carried in Construct
P{UAS-ttk.p69}
Also Known As
UAS-ttk69
Key Links
Transgenic product class
cDNA
(Giesen et al., 1997)
wild_type
(Giesen et al., 1997)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Giesen et al., 1997)
wild_type
(Giesen et al., 1997)
Mutagen
in vitro construct
(Giesen et al., 1997)
Progenitor genotype
Carried in construct
P{UAS-ttk.p69}
Cytology
Description

UASt regulatory sequences drive expression of a cDNA clone expressing the p69 isoform of ttk.

(Giesen et al., 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ttk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal neuroanatomy, with Scer\GAL4c739

      (Nicolai et al., 2003)

      abnormal oviposition, with Scer\GAL4GMR44E10

      (Knapp et al., 2019)

      decreased cell size | somatic clone, with Scer\GAL4e22c

      (Boyle and Berg, 2009)

      lethal, with Scer\GAL4cb01

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb04

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb06

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb07

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb08

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb09

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb10

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb13

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb15

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb20

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb21

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb22

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb24

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb26

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb28

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb29

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb30

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb32

      (Ward et al., 2002)

      lethal, with Scer\GAL4cb39

      (Ward et al., 2002)

      lethal | female, with Scer\GAL4GMR44E10

      (Knapp et al., 2019)

      lethal - all die during embryonic stage, with Scer\GAL4how-24B, Scer\GAL4twi.PB

      (Ciglar et al., 2014)

      viable, with Scer\GAL4cb03

      (Ward et al., 2002)

      viable, with Scer\GAL4cb05

      (Ward et al., 2002)

      viable, with Scer\GAL4cb11

      (Ward et al., 2002)

      viable, with Scer\GAL4cb12

      (Ward et al., 2002)

      viable, with Scer\GAL4cb17

      (Ward et al., 2002)

      viable, with Scer\GAL4cb19

      (Ward et al., 2002)

      viable, with Scer\GAL4cb23

      (Ward et al., 2002)

      viable, with Scer\GAL4cb25

      (Ward et al., 2002)

      viable, with Scer\GAL4cb27

      (Ward et al., 2002)

      viable, with Scer\GAL4cb31

      (Ward et al., 2002)

      viable, with Scer\GAL4cb34

      (Ward et al., 2002)

      viable, with Scer\GAL4cb35

      (Ward et al., 2002)

      viable, with Scer\GAL4cb36

      (Ward et al., 2002)

      viable, with Scer\GAL4cb37

      (Ward et al., 2002)

      viable, with Scer\GAL4cb38

      (Ward et al., 2002)

      viable, with Scer\GAL4cb40

      (Ward et al., 2002)

      visible, with Scer\GAL4Eq

      (Pi et al., 2001)

      visible, with Scer\GAL4ey.PB

      (Kumar and Moses, 2001)
      Phenotype Manifest In

      adult mushroom body, with Scer\GAL4c739

      (Nicolai et al., 2003)

      adult subretinal glial cell, with Scer\GAL4c527

      (Hummel et al., 2002)

      cone cell, with Scer\GAL4GMR.PF

      (Li et al., 1997)

      cone cell | third instar larval stage, with Scer\GAL4GMR.PF

      (Siddall et al., 2009)

      cone cell | third instar larval stage, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)

      egg chamber | oogenesis stage S14, with Scer\GAL4GMR44E10

      (Knapp et al., 2019)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL4btl.PS

      (Araujo et al., 2007)

      embryonic dorsal vessel, with Scer\GAL4how-24B, Scer\GAL4twi.PB

      (Ciglar et al., 2014)

      embryonic somatic muscle cell, with Scer\GAL4how-24B, Scer\GAL4twi.PB

      (Ciglar et al., 2014)

      embryonic visceral muscle cell, with Scer\GAL4how-24B, Scer\GAL4twi.PB

      (Ciglar et al., 2014)

      eye, with Scer\GAL4ey.PB

      (Kumar and Moses, 2001)

      eye, with Scer\GAL4GMR.PF

      (Siddall et al., 2009, Li et al., 1997)

      eye, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)

      eye disc morphogenetic furrow, with Scer\GAL4dpp.blk1

      (Kumar and Moses, 2001)

      eye photoreceptor cell, with Scer\GAL4GMR.PF

      (Li et al., 1997)

      interommatidial bristle, with Scer\GAL4GMR.PF

      (Li et al., 1997)

      Kenyon cell, with Scer\GAL4c739

      (Nicolai et al., 2003)

      lamina anlage, with Scer\GAL4c527

      (Hummel et al., 2002)

      lamina anlage glial cell, with Scer\GAL4c527

      (Hummel et al., 2002)

      microchaeta, with Scer\GAL4Eq

      (Pi et al., 2001)

      ommatidium, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)

      ommatidium | third instar larval stage, with Scer\GAL4GMR.PF

      (Siddall et al., 2009)

      oocyte | oogenesis stage S10B | somatic clone, with Scer\GAL4e22c

      (Boyle and Berg, 2009)

      peripheral nervous system, with Scer\GAL4Kr.PM

      (Giesen et al., 1997)

      photoreceptor cell R1 | third instar larval stage, with Scer\GAL4GMR.PF

      (Siddall et al., 2009)

      photoreceptor cell R1 | third instar larval stage, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)

      photoreceptor cell R6 | third instar larval stage, with Scer\GAL4GMR.PF

      (Siddall et al., 2009)

      photoreceptor cell R6 | third instar larval stage, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)

      photoreceptor cell R7 | third instar larval stage, with Scer\GAL4GMR.PF

      (Siddall et al., 2009)

      photoreceptor cell R7 | third instar larval stage, with Scer\GAL4lz-gal4

      (Siddall et al., 2009)
      Detailed Description
      Statement
      Reference

      Adulthood-specific expression of ttkp69.UAS under the control of Scer\GAL4GMR44E10 leads to females with a severely decreased egg-laying rate and significant retention of mature follicles in the ovary, as compared to controls. Consistently, follicles rupture much less frequently upon induction by OA or ionomycin, as compared to controls.

      (Knapp et al., 2019)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4twi.PB and Scer\GAL4how-24B leads to lethality at embryonic stages due to severe defects in the specification of all three muscle types. The heart is either completely absent or only a few isolated cardioblasts are visible, visceral musculature contains gaps and has abnormal organization, somatic musculature contains very few correctly specific and differentiated muscle fibers, with cells that remain mononucleated and form small muscles that lack clear identity.

      (Ciglar et al., 2014)

      Follicular cells expressing ttkp69.Scer\UAS under the control of Scer\GAL4e22c are reduced in size.

      (Boyle and Berg, 2009)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4lz-gal4 results in severely disrupted adult eyes that show scarring and lack ommatidial structures. In third instar larval eye discs ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF results in severely perturbed adult eyes, displaying no ommatidial structure. Ommatidia show reduced numbers of cone cells and R1, R6 and R7 photoreceptor cells.

      (Siddall et al., 2009)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4btl.PS results in impaired tracheal branch fusion.

      (Araujo et al., 2007)

      When expression is driven by Scer\GAL4c739, ttkp69.Scer\UAS shows a mushroom body Class I phenotype; neuron number reduced. This phenotype is similar to, but less extreme than, that caused by ttkUY181 driven by Scer\GAL4c739. Loss of neurons is not accompanied by increase in number of glial cells.

      (Nicolai et al., 2003)

      When Scer\GAL4c527 is expressed under the control of Scer\GAL4c527 in third instar larvae, defects are seen in glial cell migration in the developing eye. In about 20% of these animals surface glial cells do not enter the eye disc and R cell axons fail to enter the optic stalk forming a mass of R cell axons in the basal region of the disc. In the remaining larvae, fewer surface glial cells are found in the eye disc. In these discs R cell axons project into the optic stalk, but are highly disorganised.

      (Hummel et al., 2002)

      Flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4cb12, Scer\GAL4cb19, Scer\GAL4cb35, Scer\GAL4cb36, Scer\GAL4cb37 or Scer\GAL4cb38 are moderately viable. Flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4cb05 or Scer\GAL4cb23 are viable at 18oC.

      (Ward et al., 2002)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4ey.PB results in deletion of the eye.

      (Kumar and Moses, 2001)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4dpp.blk1 affects furrow reincarnation but not birth in the eye disc.

      (Kumar and Moses, 2001)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4Eq1 results in a lack of most microchaetae.

      (Pi et al., 2001)

      Scer\GAL4Kr.PM-mediated expression leads to a partial block of neuronal development in the PNS and CNS. Scer\GAL4sca-537.4-mediated expression impairs neuronal differentiation. In late stage 16/17 embryos cell death is observed.

      (Giesen et al., 1997)

      Eyes are deformed, severely reduced in size and lack bristles, cone cells and photoreceptor cells in flies expressing ttkp69.Scer\UAS under the control of Scer\GAL4GMR.PF.

      (Li et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Over-expression of lzGMR.PD in flies expressing ttkp69.Scer\UAS in under the control of Scer\GAL4GMR.PF partially suppresses the loss cone cells and R1, R6 and R7 photoreceptor cells.

      (Siddall et al., 2009)

      Co-expression of repoScer\UAS.cYa and ttkp69.Scer\UAS under the control of Scer\GAL4sca-T3 results in a synergistic suppression of neuronal development in embryos.

      (Yuasa et al., 2003)

      Most notal microchaetae are missing in animals coexpressing phylScer\UAS.cPa and ttkp69.Scer\UAS under the control of Scer\GAL4Eq1.

      (Pi et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      ttkp69.UAS/Scer\GAL4btl.PS rescues ttkD2-50

      (Araujo et al., 2007)
      Partially rescues

      ttkp69.UAS, Scer\GAL4twi.PB, Scer\GAL4how-24B partially rescues ttkD2-50

      (Ciglar et al., 2014)

      ttkp69.UAS/Scer\GAL4hs.PB partially rescues ttktwp/ttk1e11

      (French et al., 2003)

      ttkp69.UAS/Scer\GAL4sli.PS partially rescues ttkB330

      (Giesen et al., 1997)
      Comments

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4twi.PB and Scer\GAL4how-24B partially rescues the muscle morphology defects of ttkD2-50 mutants.

      (Ciglar et al., 2014)

      Expression of ttkp69.Scer\UAS under the control of Scer\GAL4btl.PS in ttkD2-50 mutant animals rescues the tracheal intercalation defects. Terminal branch fusion defects of the lateral trunk are also rescued in these animals.

      (Araujo et al., 2007)

      Short heat shocks and moderate temperatures in ttktwp/ttktwp, ttkp69.Scer\UAS Scer\GAL4hs.PB animals lead to significant partial rescue of dorsal appendage and chorion defects. Eggs are normal in shape and size and dorsal appendages are significantly longer than seen in the mutants alone. Longer heat shocks lead to a dramatic decline in rescue frequency accompanied by an increase in defects early in oogenesis.

      (French et al., 2003)

      Scer\GAL4sli.PS-mediated expression partially rescues the CNS phenotype with two copies of the construct.

      (Giesen et al., 1997)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ttkp69.Scer\UAS
      ttkp69.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (21)