FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\phylUAS.cPa
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General Information
Symbol
Dmel\phylUAS.cPa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Pi
FlyBase ID
FBal0126345
Feature type
allele
Associated gene
Dmel\phyl
Associated Insertion(s)
Carried in Construct
P{UAS-phyl.P}
Also Known As
UAS-phyl
Key Links
Transgenic product class
wild_type
(Pi et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Pi et al., 2001)
Mutagen
in vitro construct
(Pi et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-phyl.P}
Cytology
Description

UAS regulatory sequences drive expression of phyl.

(Pi et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
phyl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      adult midgut epithelium, with Scer\GAL4NP5130

      (Yin and Xi, 2018)

      adult midgut epithelium, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      adult midgut epithelium | cell autonomous, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      adult midgut epithelium | cell non-autonomous, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      adult midgut epithelium | somatic clone, with Scer\GAL4Act.PU

      (Yin and Xi, 2018)

      enteroblast | adult stage, with Scer\GAL4NP5130

      (Yin and Xi, 2018)

      enteroendocrine cell | adult stage, with Scer\GAL4NP5130

      (Yin and Xi, 2018)

      enteroendocrine cell | adult stage, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      enteroendocrine cell | adult stage | cell autonomous, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      enteroendocrine cell | adult stage | cell non-autonomous, with Scer\GAL4Su(H).GBE

      (Yin and Xi, 2018)

      enteroendocrine cell | adult stage | somatic clone, with Scer\GAL4Act.PU

      (Yin and Xi, 2018)

      external sensory organ | ectopic, with Scer\GAL4dpp.blk1

      (Pi et al., 2011)

      external sensory organ | ectopic, with Scer\GAL4Eq

      (Pi et al., 2011)

      intestinal stem cell | adult stage, with Scer\GAL4NP5130

      (Yin and Xi, 2018)

      intestinal stem cell | adult stage | somatic clone, with Scer\GAL4Act.PU

      (Yin and Xi, 2018)

      mesonotum, with Scer\GAL4dpp.blk1

      (Pi et al., 2011)

      mesonotum, with Scer\GAL4Eq

      (Pi et al., 2011)

      mesothoracic tergum & microchaeta | ectopic, with Scer\GAL4Eq

      (Pi et al., 2001)

      microchaeta, with Scer\GAL4sca-109-68

      (Pi et al., 2001)

      muscle cell of A1-7 dorsal transverse muscle 1, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB

      (Artero et al., 2003)

      muscle cell of A1-7 lateral longitudinal muscle 1, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB

      (Artero et al., 2003)

      muscle cell of A1-7 lateral transverse muscle 4, with Scer\GAL4Mef2.PR, Scer\GAL4twi.PB

      (Artero et al., 2003)

      neuron | increased number, with Scer\GAL4sca-109-68

      (Pi et al., 2001)

      scutellar bristle | ectopic, with Scer\GAL4dpp.blk1

      (Pi et al., 2001)

      sensory neuron | increased number, with Scer\GAL4sca-537.4

      (Pi et al., 2001)

      thecogen cell | increased number, with Scer\GAL4sca-109-68

      (Pi et al., 2001)

      tormogen cell, with Scer\GAL4sca-109-68

      (Pi et al., 2001)

      trichogen cell, with Scer\GAL4sca-109-68

      (Pi et al., 2001)

      wing vein L3 & chaeta | ectopic, with Scer\GAL4dpp.blk1

      (Pi et al., 2001)
      Detailed Description
      Statement
      Reference

      Adulthood-only expression of phylScer\UAS.cPa under the control of Scer\GAL4esg-NP5130 (using Gal80[ts] for the temporal control of expression) leads to a severe increase in the number of enteroendocrine cells and to a small but significant increase in the intestinal stem cell and enteroblast population in the adult midgut, as compared to controls; after 2 weeks, this expression leads to the development of multilayered tumors of enteroendocrine cell-like cells. Adulthood-only expression under the control of Scer\GAL4Dl-05151-G or Scer\GAL4Su(H).GBE (also using Gal80[ts]) also leads to a severe or small increase, respectively, in the number of enteroendocrine cells in the adult midgut and in the number of mitotic cells, as compared to controls; phylScer\UAS.cPa, Scer\GAL4Su(H).GBE cells are significantly more numerous and lack enteroendocrine-fate markers, as compared to Scer\GAL4Su(H).GBE-only controls.

      Clonal expression of phylScer\UAS.cPa under the control of Scer\GAL4Act.PU in the adult midgut leads to the clones exhibiting severe increases in the proportion of enteroendocrine cells and intestinal stem cells, as well as mitotic cells, as compared to control clones and neighboring control tissue.

      (Yin and Xi, 2018)

      Overexpression of phylScer\UAS.cPa by Scer\GAL4Eq1 induces ectopic external sensory organs on the notum, in particular, in the midline region.

      Expression of phylScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 has no effect on the morphology of the L3 vein.

      (Pi et al., 2011)

      Expression of phylScer\UAS.cPa under the control of both Scer\GAL4twi.PB and Scer\GAL4Mef2.PR results in alterations in muscle identities in embryos. Muscles DT1 and LT4 consistently have altered morphology and muscle LL1 is occasionally affected. Muscle DT1 fails to attach normally, resulting in a "ball of muscle" phenotype. LT4 fails to grow dorsally, resulting in a smaller muscle than normal.

      (Artero et al., 2003)

      Expression of phylScer\UAS.cPa under the control of Scer\GAL4Eq1 results in ectopic bristles on the notum; there is a 30-70% increase in the number of notal microchaetae, with a highest density in the midline region. Expression of phylScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 results in ectopic bristles on the scutellum and third wing vein. Supernumerary sensory neurons are produced in the embryo when phylScer\UAS.cPa is expressed under the control of Scer\GAL4sca-537.4. This increase in the number of neurons is due to transformation of outer support cells. Expression of phylScer\UAS.cPa under the control of Scer\GAL4sca-109-68 results in microchaetae in which the outer hair and socket structures are missing and have been transformed into neurons and sheath cells.

      (Pi et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by chnC05-441/Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, enhanceable by chnC05-441/Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has mesonotum phenotype, enhanceable by talUAS.cPa/Scer\GAL4dpp.blk1

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by talORF3.UAS/Scer\GAL4dpp.blk1

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has mesonotum phenotype, enhanceable by talORF3.UAS/Scer\GAL4dpp.blk1

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by talC05-660/Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, enhanceable by talC05-660/Scer\GAL4Eq

      (Pi et al., 2011)

      phylUAS.cPa has wing vein L3 phenotype, enhanceable by Scer\GAL4dpp.blk1/talORF1.UAS

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by Scer\GAL4Eq/talUAS.cPa

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, enhanceable by Scer\GAL4Eq/talUAS.cPa

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by Scer\GAL4dpp.blk1/talORF1.UAS

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has mesonotum phenotype, enhanceable by Scer\GAL4dpp.blk1/talORF1.UAS

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has external sensory organ | ectopic phenotype, enhanceable by talUAS.cPa/Scer\GAL4dpp.blk1

      (Pi et al., 2011)
      NOT Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4NP5130, phylUAS.cPa has enteroendocrine cell | adult stage phenotype, suppressible by sinaKK107603/Scer\GAL4NP5130

      (Yin and Xi, 2018)

      Scer\GAL4NP5130, phylUAS.cPa has intestinal stem cell | adult stage phenotype, suppressible by sinaKK107603/Scer\GAL4NP5130

      (Yin and Xi, 2018)

      Scer\GAL4NP5130, phylUAS.cPa has enteroblast | adult stage phenotype, suppressible by sinaKK107603/Scer\GAL4NP5130

      (Yin and Xi, 2018)

      Scer\GAL4NP5130, phylUAS.cPa has adult midgut epithelium phenotype, suppressible by sinaKK107603/Scer\GAL4NP5130

      (Yin and Xi, 2018)

      Scer\GAL4NP5130, phylUAS.cPa has enteroendocrine cell | adult stage phenotype, suppressible by Scer\GAL4NP5130/ttkUAS.Tag:FLAG

      (Yin and Xi, 2018)

      Scer\GAL4NP5130, phylUAS.cPa has adult midgut epithelium phenotype, suppressible by Scer\GAL4NP5130/ttkUAS.Tag:FLAG

      (Yin and Xi, 2018)

      Scer\GAL4Act.PU, phylUAS.cPa has enteroendocrine cell | somatic clone | adult stage phenotype, suppressible by Df(1)sc-B57/Df(1)sc-B57

      (Yin and Xi, 2018)

      Scer\GAL4Act.PU, phylUAS.cPa has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by Df(1)sc-B57/Df(1)sc-B57

      (Yin and Xi, 2018)

      Scer\GAL4Act.PU, phylUAS.cPa has adult midgut epithelium | somatic clone phenotype, suppressible by Df(1)sc-B57/Df(1)sc-B57

      (Yin and Xi, 2018)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, suppressible by Scer\GAL4Eq/dbrC05-544

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, suppressible by Scer\GAL4Eq/dbrC05-544

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has external sensory organ | ectopic phenotype, suppressible by Scer\GAL4dpp.blk1/tal1-4FS.UAS

      (Pi et al., 2011)

      Scer\GAL4dpp.blk1, phylUAS.cPa has mesonotum phenotype, suppressible by Scer\GAL4dpp.blk1/tal1-4FS.UAS

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, suppressible by E(spl)m8-HLHC05-346, Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, suppressible by E(spl)m8-HLHC05-346, Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, suppressible by Scer\GAL4Eq/esgA02-050

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, suppressible by Scer\GAL4Eq/esgA02-050

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has external sensory organ | ectopic phenotype, suppressible by CG10588C05-439, Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesonotum phenotype, suppressible by CG10588C05-439, Scer\GAL4Eq

      (Pi et al., 2011)

      Scer\GAL4Eq, phylUAS.cPa has mesothoracic tergum & microchaeta | ectopic phenotype, suppressible by ttkp69.UAS/Scer\GAL4Eq

      (Pi et al., 2001)
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased numbers of adult midgut enteroendocrine cells, intestinal stem cells and enteroblasts resulting from the adulthood-only expression of phylScer\UAS.cPa under the control of Scer\GAL4esg-NP5130 (using Gal80[ts] for the temporal control of expression) is suppressed by the co-expression of sinaKK107603. The increased number of enteroendocrine cells is also suppressed by the co-expression of ttkScer\UAS.T:Zzzz\FLAG.

      The increased number of enteroendocrine cells in adult midgut clones expressing phylScer\UAS.cPa under the control of Scer\GAL4Act.PU is suppressed by Df(1)sc-B57 homozygosity; these rescued clones are composed of small progenitor cells.

      The co-expression of phylScer\UAS.cPa partially suppresses the small size and cell number of adult midgut clones expressing scScer\UAS.cPa under the control of Scer\GAL4Act.PU.

      (Yin and Xi, 2018)

      The presence of chnEPC05-441 enhances the phylScer\UAS.cPa (under the control of Scer\GAL4Eq1) overexpression phenotype.

      Expression of E(spl)m8-HLHEPC05-346 and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 leads to missing external sensory organs.

      Expression of esgEPA02-050 and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 leads to missing external sensory organs.

      Expression of CG10588EPC05-439 and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 leads to missing external sensory organs.

      Expression of dbrEPC05-544 and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 leads to a disorganised external sensory organ pattern.

      Co-expression of talScer\UAS.cPa and phylScer\UAS.cPa in the wing disc under the control of Scer\GAL4dpp.blk1 causes strong L3 vein expansion that is accompanied by ectopic external sensory organ formation.

      Co-expression of talScer\UAS.cPa and phylScer\UAS.cPa under the control of Scer\GAL4dpp.blk1 induces approximately 7.4 (+/- 1.5) ectopic external sensory organs.

      Co-expression of tal1-4FS.Scer\UAS does not enhance the phylScer\UAS.cPa ectopic external sensory organ phenotype (when both are expressed by Scer\GAL4dpp.blk1).

      Co-expression of tal1-4FS.Scer\UAS and phylScer\UAS.cPa in the wing disc under the control of Scer\GAL4dpp.blk1 does not affect wing vein and external sensory organ formation.

      Co-expression of talORF1.Scer\UAS with phylScer\UAS.cPa, both under the control of Scer\GAL4dpp.blk1, induces as many as 7.2 (+/- 1.4) ectopic external sensory organs.

      Co-expression of talORF3.Scer\UAS with phylScer\UAS.cPa, both under the control of Scer\GAL4dpp.blk1, induces as many as 9.6 (+/- 2.5) ectopic external sensory organs.

      Expression of talEPC05-660 and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 leads to an increase in external sensory organ density (more than upon expression of phylScer\UAS.cPa alone).

      Co-expression of talORF1.Scer\UAS with phylScer\UAS.cPa, both under the control of Scer\GAL4dpp.blk1, results in strong L3 wing vein expansion and formation of a large number of external sensory organs on the L3 vein.

      Co-expression of talScer\UAS.cPa and phylScer\UAS.cPa under the control of Scer\GAL4Eq1 induces ectopic external sensory organs on the notum.

      (Pi et al., 2011)

      Most notal microchaetae are missing in animals coexpressing phylScer\UAS.cPa and ttkp69.Scer\UAS under the control of Scer\GAL4Eq1.

      (Pi et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      phylScer\UAS.cPa
      phylUAS.cPa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Pi
      (Pi et al., 2001)
      Secondary FlyBase IDs
        References (5)