FlyBase Allele Report: Dmel\par-1[k06323]

Germline stem cells (GSCs) in newly eclosed par-1^k06323/par-1^k05603, par-1^k06323/par-1^W3 and par-1^k06323/Df(2R)BSC22 males show a high frequency of centrosome misorientation and frequently undergo mitosis with misoriented spindles (mitosis with misoriented spindles is not seen in wild-type male GSCs).

(Yuan et al., 2012)

Border follicle cell migration is disrupted in par-1^k06323/par-1^27C1, par-1^k06323/par-1^70D3 and par-1^k06323/par-1^W3 females.

par-1^k06323/par-1^70D3 border follicle cells extend protrusions as occurs in wild-type border cells, but in contrast to wild-type cells, where the protrusions are extended mainly in the forward direction (in the direction of migration), in the mutant cells forward protrusions are decreased at the expense of backward and lateral protrusions.

par-1^k06323/par-1^27C1 border follicle cells that fail to detach from the anterior of the egg chamber have longer protrusions on average than wild-type border follicle cells. In addition , one to three cell fragments are often seen along the migration pathway in the mutant egg chambers.

(McDonald et al., 2008)

A small fraction of par-1^Δ-16/par-1^k06323 animals survive to late larval stages. Some boutons at the neuromuscular junction of muscle 6/7 are smaller than normal and irregularly shaped in these mutant larvae, and the number of boutons is slightly, but significantly increased compared to normal. The subsynaptic reticulum (SSR) is expanded in the mutant type I boutons, with the overall SSR to bouton area ratio being higher than that of controls.

Homozygous larvae show a slight increase in the number of boutons at the neuromuscular junction compared to controls.

The frequency of the miniature excitatory junctional current is slightly increased in par-1^k06323/par-1^Δ-16 animals compared to controls, while the amplitude is not significantly altered. The amplitude of the evoked excitatory junctional current is increased in the mutant animals compared to controls, and the quantal content is also significantly increased.

(Zhang et al., 2007)

Stage 9 oocytes from par-1^k06323/par-1^k06821b females have mild polarity defects

(Zimyanin et al., 2007)

par-1^k06323/par-1^W3 animals show loss of oocyte polarity.

(Martin and St. Johnston, 2003)

Embryos derived from par-1^k06323/par-1^k05603 females have an average of 19 pole cells/embryo (compared to 33 in the wild-type), and 12% of the embryos lack a germline.

(Riechmann et al., 2002)

par-1^W3/par-1^k05603 oocytes show misorganisation of the microtubule network.

(Hachet and Ephrussi, 2001)

The progeny of homozygous females show typical posterior group phenotypes; the embryos may lack abdominal segments (only 44% of embryos and hatched larvae have the wild-type abdominal pattern and the average number of denticle belts is 5.0) and pole cells may fail to form, giving rise to a grandchildless phenotype in which adult escapers have agametic gonads (10/16 of adult escapers have germ cells). The progeny of par-1^k06821b/par-1^k06323 females show typical posterior group phenotypes; the embryos may lack abdominal segments (only 73% of embryos and hatched larvae have the wild-type abdominal pattern and the average number of denticle belts is 6.9) and pole cells may fail to form, giving rise to a grandchildless phenotype in which adult escapers have agametic gonads (69/94 of adult escapers have germ cells). The progeny of par-1^W3/par-1^k06323 females show typical posterior group phenotypes; the embryos lack abdominal segments (only 5% of embryos and hatched larvae have the wild-type abdominal pattern and the average number of denticle belts is 0.7) and pole cells fail to form, giving rise to a grandchildless phenotype in which adult escapers have agametic gonads. Migration of the nucleus to the anterior occurs normally in par-1^W3/par-1^k06323 oocytes.

(Shulman et al., 2000)

20% of embryos derived from par-1^k05603/par-1^k06323 and par-1^k06323/par-1^k06323 females die, showing defects which range from disturbances in posterior patterning to complete absence of abdominal segments.

(Tomancak et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

par-1^k06323/par-1[+] is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

NOT Suppressor of

Statement

Reference

par-1^k06323/par-1[+] is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^Q108.UAS

(Ambegaokar and Jackson, 2011)

Other

Statement

Reference

PKD^cl4, par-1^k06323 has viable phenotype

(Maier et al., 2019)

Scer\GAL4^{VP16.mat.αTub67C}, osk^UASp.cZa, par-1^k06323/par-1^k06821b has sterile phenotype

(Zimyanin et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

par-1^k06323 has oocyte | oogenesis stage S9 phenotype, enhanceable by Scer\GAL4^{VP16.mat.αTub67C}/osk^UASp.cZa

(Zimyanin et al., 2007)

Suppressed by

Statement

Reference

par-1^k06323/par-1^W3 has oocyte phenotype, suppressible | partially by Lkb1^UASp.GFP/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St Johnston, 2003)

NOT suppressed by

Statement

Reference

par-1^k06323/par-1^W3 has oocyte phenotype, non-suppressible by Lkb1^{K147M.UASp.GFP}/Scer\GAL4^{VP16.mat.αTub67C}

(Martin and St Johnston, 2003)

Enhancer of

Statement

Reference

par-1^k06323/par-1[+] is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Suppressor of

Statement

Reference

par-1^k06323/par-1[+] is a suppressor | partially of egg chamber phenotype of RhoGAP19D^unspecified

(Fic et al., 2021)

NOT Suppressor of

Statement

Reference

par-1^k06323/par-1[+] is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^Q108.UAS

(Ambegaokar and Jackson, 2011)

Other

Statement

Reference

osk¹, par-1^k05603/par-1^k06323 has primordial germ cell phenotype

(Riechmann et al., 2002)

osk¹, par-1^k05603/par-1^k06323 has germline cell phenotype

(Riechmann et al., 2002)

osk[+]/osk¹, par-1^k05603/par-1^k06323 has primordial germ cell phenotype

(Riechmann et al., 2002)

osk[+]/osk¹, par-1^k05603/par-1^k06323 has germline cell phenotype

(Riechmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of par-1^k06323 does not suppress the apoptosis seen in flies overexpressing hid.

(Ambegaokar and Jackson, 2011)

The mild polarity defects seen in stage 9 oocytes from par-1^k06323/par-1^k06821b females are enhanced by osk^{Scer\UAS.P\T.cZa}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}. The resulting females do not lay any eggs because of severe defects in oogenesis.

(Zimyanin et al., 2007)

lkb1^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} partially rescues the loss of anterior-posterior polarity seen in the oocytes of par-1^k06323/par-1^W3 animals. lkb1^{K147M.Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} does not rescue the loss of anterior-posterior polarity seen in the oocytes of par-1^k06323/par-1^W3 animals.

(Martin and St Johnston, 2003)

Embryos derived from par-1^k06323/par-1^k05603 ; osk¹/+ females have an average of 3 pole cells/embryo (compared to 33 in the wild-type), and 42% of the embryos lack a germline.

(Riechmann et al., 2002)

Xenogenetic Interactions

Statement

Reference

One copy of par-1^k06323 enhances the rough eye phenotype seen in flies expressing Hsap\MAPT^GMR.Ex.PJ.

par-1^k06323 does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

One copy of par-1^k06323 does not suppress the rough eye phenotype seen in flies expressing Zzzz\CAG^{Q108.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

(Ambegaokar and Jackson, 2011)

Complementation and Rescue Data

Rescued by

par-1^k06323/par-1^W3 is rescued by par-1^{N1L.αTub67C.mGFP6}

(Doerflinger et al., 2006)

par-1^k06323/par-1^W3 is rescued by par-1^{N2S.αTub67C.mGFP6}

(Doerflinger et al., 2006)

par-1^k06323/par-1^W3 is rescued by par-1^{N3L.αTub67C.mGFP6}

(Doerflinger et al., 2006)

par-1^k06323/par-1^W3 is rescued by Scer\GAL4^CY2/par-1^{N1S.UASp.mGFP6}

(Doerflinger et al., 2006)

par-1^k06323/par-1^W3 is rescued by par-1^{N1S.αTub67C.GFP}

(Shulman et al., 2000)

Comments

Expression of par-1^{N1L.αTub67C.T:Avic\GFP-m6} in the viable transheterozygote par-1^k06323/par-1^W3 fully rescues the posterior localisation of osk mRNA.

Expression of par-1^{N2S.αTub67C.T:Avic\GFP-m6} in the viable transheterozygote par-1^k06323/par-1^W3 partially rescues (approximately 30% mislocalisation) the posterior localisation of osk mRNA.

Expression of par-1^{N3L.αTub67C.T:Avic\GFP-m6} in the viable transheterozygote par-1^k06323/par-1^W3 partially rescues (approximately 40% mislocalisation) the posterior localisation of osk mRNA.

Expression of par-1^{N1S.Scer\UAS.P\T.T:Avic\GFP-m6} in the viable transheterozygote par-1^k06323/par-1^W3 fully rescues the posterior localisation of osk mRNA.

(Doerflinger et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10615

y¹ w^67c23; P{lacW}par-1^k06323/CyO

Kyoto

111191

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}par-1^k06323 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Separable from: a lethal mutation on the chromosome.

(Tomancak et al., 2000)

Comments

Complements: mei-W68¹.

(Shulman et al., 2000)

Excision of the P{lacW} element can completely revert the oogenesis phenotype.

(Tomancak et al., 2000)

Complements: hts⁰⁰⁶³⁴. Complements: hts⁰¹¹⁰³. Complements: l(2)k00705^k00705. Complements: l(2)k00705^k05105. Complements: hts^k06121. Complements: l(2)k00705^k08203. Complements: l(2)k00705^k10233. Complements: l(2)k00705^k11202. Complements: RpL11^k16914.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

l(2)k06323

(Cox et al., 2001, Shulman et al., 2000, Tomancak et al., 2000, Palmer et al., 1999, Spradling et al., 1999)

l(2)k06323^k06323

(BDGP Project Members, 1994-1999)

par-1⁶³²³

(Doerflinger et al., 2022, Fic et al., 2021, Baskar et al., 2019, Jouette et al., 2019, Nashchekin et al., 2016, Morais-de-Sá et al., 2013, Sanghavi et al., 2012, Doerflinger et al., 2010, Gonsalvez et al., 2010, McDonald et al., 2008, Zimyanin et al., 2007, Zimyanin et al., 2007, Lin et al., 2006, Benton and St. Johnston, 2003, Martin and St. Johnston, 2003, Martin and St Johnston, 2003, Shulman et al., 2000)

par-1^9A

(Coutelis and Ephrussi, 2007, Zhang et al., 2007, Riechmann and Ephrussi, 2004, Riechmann et al., 2002, Tomancak et al., 2000)

par-1^K06323

(Deichsel et al., 2024, Deichsel et al., 2024, Yuan et al., 2012)

par-1^k06323

(Datta et al., 2023, Titus et al., 2023, Maier et al., 2019, Ambegaokar and Jackson, 2011)

par-1^l(2)k06323

(Benton et al., 2002)

par1⁶³²³

(Munro et al., 2006)

par1^9A

(Krauss et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (38)

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